Tài liệu Báo cáo khoa học: Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium doc

... (2005) 2869–2877 ª 2005 FEBS 2877 Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium Kiyohiko Igarashi 1 , ... These results clearly indicate that the first and second electrons of two -electron- reduced CDH are both transferred via heme, and that the redox react...

Ngày tải lên: 19/02/2014, 18:20

... out in the presence of one of the substrates of the enzyme. The physiological electron acceptor of the A. ful- gidus enzyme is still unknown. Therefore, no substrate could be added to the titration ... oxidation of the heme groups of the enzyme by various compounds was tested to identify the physiological electron acceptor of the enzyme. The enzyme was...

Ngày tải lên: 21/02/2014, 03:20

... designating the extent of the protein interactions: the growth rate of cotransformed Y190 yeast colonies (activation of HIS3 reporter); the duration of the development of blue color in the LacZ ... observed in the filter-lift assay, due to the quantifiable nature of the liquid assays. Also, the mag- nitude of the decrease in interaction between SmSmad1 and Sm...

Ngày tải lên: 18/02/2014, 16:20

... Measuring the rate and extent of back electron transfer from the ferrous NOS heme to FMNsq following flash photolysis of CO can indicate precisely the rate of electron transfer, but can- not reveal the ... K eq position for maximum electron flux through the enzyme. On either side of this optimum, the electron flux drops off because either the formation rate (k...

Ngày tải lên: 18/02/2014, 11:20

... [48]. Interaction of FNR with the NADP + coenzyme and the hydride transfer event Once the FAD cofactor of FNR has accepted two electrons, they have to be transferred to NADP + . The FNR protein ... and the C- terminal Y303 in AnFNR. The two one -electron midpoint potentials of the fla- vin in FNRs are close to each other, therefore these proteins stabilize only 10–20% o...

Ngày tải lên: 18/02/2014, 11:20

... either no nucleotide cofactor or had slowly hydrolysable analogues of ATP. The rate enhancement by the presence of ATP was in the order of about 15-fold (Fig. 6), strongly suggesting that the hydrolysis ... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) wit...

Ngày tải lên: 19/02/2014, 07:20

... substrates that the C1-C2 reactive bond of the steroid substrate is inappro- priately aligned with the flavin N5 atom to enable hydride transfer from the flavin in the reduced form of the enzyme [2]. ... grossly perturb the overall framework of the enzyme or the active site. The active sites of the enzymes are shown in stick format with the sigmaA weigh- ted...

Ngày tải lên: 20/02/2014, 02:21

... system). The results are shown in Table 4. Here, the accuracy of the baseline system is #1 (the number of target sentences of the most major cluster) divided by #1&2 (the number of target ... such as the role of the speaker of the sentence (Yamada et al., 2000) (also, the topic that sentences are referring to) may be effective; and (2) Considering th...

Ngày tải lên: 20/02/2014, 18:20

... reduced from 19 to 16 min and the half-time of the slow phase was reduced from 270 to 154 min. In the presence of unlabeled PCSK9- D374Y, the half-time of the rapid phase of [ 125 I]TC- PCSK9-D374Y ... PCSK9-S127R, only the upper and lower, respectively, halves of the ranges are shown, to avoid overlap of the error bars. The results are expressed as percentag...

Ngày tải lên: 14/02/2014, 14:20

... [46]. The similarity in the K m values is attributable to the uracil moiety binding in the same fashion [38], which could trigger the initial binding of both substrates. In UpG, the binding of the guanine ... secretory RNases from the ze- brafish share the RNase A scaffold [7]. Small-molecule inhibitors of these RNases could be used to investigate their broad biologic...

Ngày tải lên: 14/02/2014, 22:20