Tài liệu Báo cáo khoa học: AcmA of Lactococcus lactis is an N-acetylglucosaminidase with an optimal number of LysM domains for proper functioning ppt

... 2854–2868 ª 2005 FEBS AcmA of Lactococcus lactis is an N-acetylglucosaminidase with an optimal number of LysM domains for proper functioning Anton Steen 1 , Girbe Buist 1 , Gavin J. Horsburgh 2 , Gerard ... and an Axion Vision camera (Axion Technologies, Houston, TX). Isolation of AcmA from the supernatant of L. lactis cultures The cells of 100 mL...

Ngày tải lên: 19/02/2014, 18:20

... (Promega). Acknowledgements We thank Sutton Mooney for critical reading of the manuscript. Financial support for this project was pro- vided by the Deutsche Forschungsgemeinschaft (DFG) grants HE3224 ⁄ 5-1 and 5-2 and Washington ... AP2 domain is criti- cal for assembly with BPMs, we took advantage of an earlier description of ap2-1 and ap2-5 mutants, in which mutation of...

Ngày tải lên: 18/02/2014, 06:20

... 1–3 and 7–10 can independently bind Man-6-P-containing ligands. Dahms et al. [19] further defined the location of Man-6-P binding sites by using mutational analysis to establish the importance of ... Barbara Switzer and the University of Nebraska Medical Cen- ter Monoclonal Antibody Facility for the production of the 9E10 antibody. We also thank Christine D. Dreis, Rosslyn Grosely an...

Ngày tải lên: 18/02/2014, 08:20

... Collier I, Bannikov G, Marmer BL, Grant GA & Goldberg GI (1995) Mechanism of cell surface activation of 72-kDa type IV collagenase. Isola- tion of the activated form of the membrane metallopro- tease. ... are the main components of ECM, and type I collagen is the most abundant form in bone and connective tissue [1]. Controlled turnover of ECM is critical for a wide vari-...

Ngày tải lên: 18/02/2014, 11:20

... reductase and can be progres- sively regenerated from the a anomer by spontaneous anomerisation. Kinetic analysis of the recombinant proteins from C. glabrata and A. aeolicus was performed by 13 C-NMR ... C. glabrata is the most important human pathogenic yeast other than Candida albicans, and the species was selected for study with the view that the enzyme might have potential as an...

Ngày tải lên: 18/02/2014, 18:20

... protein translocation channel at the outer envelope membrane of chloroplasts (Toc75) is synthesized as a larger precursor with an N-terminal transit peptide. Within the transit peptide of the pea ... inner envelope membrane or thylakoids), and the outer envelope membrane where trypsin can digest proteins. This targeting pattern was statistically indistinguish- able from that of an...

Ngày tải lên: 19/02/2014, 07:20

... defined changes in the nucleotide sequence and ⁄ or secondary structure of a specific RNA are planned on the basis of a preconceived idea. This requires detailed structural and mechanistic informa- tion ... control. Double bands result from an isomeric mixture (cis- ⁄ trans-isomers) of the Cy5 moiety used for labelling. (B) Schematic presentation of HP–TWRJ mediated fragment exchang...

Ngày tải lên: 20/02/2014, 01:20

... periplasmic loop of KdpD required SecE and YidC for efficient translocation (Figs 3B and 4B). This indicates that the multispanning membrane protein actually translocates in pairs of transmembrane helices and ... with antiserum to M13 coat protein (upper panel) and with antiserum to KdpD, respectively (lower panel). Ó FEBS 2003 KdpD membrane insertion (Eur. J. Biochem. 270) 1727 memb...

Ngày tải lên: 20/02/2014, 11:20

... lane e). The abundance of the upper band relative to the lower band of the enzymeÆreactivase complex formed from apoenzyme was significantly larger than that formed from the Fig. 4. Analysis of ... Mechanism of action of adenosylcobalamin: glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase – kinetics, stereospecificity, and mechanism. B...

Ngày tải lên: 15/02/2014, 01:20

... (A) and MALDI-TOF MS (B) analysis of the peptide maturation catalyzed by WT anSMEbt and by M 1 ,M 2 and M 3 mutants of anSMEbt. The WT and mutant forms of anSMEbt (each 60 l M) were incubated with ... S6. MALDI-TOF MS analysis of 17C peptide after incubation with wild type (WT) or M 1 ,M 2 and M 3 mutants of anSMEbt. Fig. S7. MALDI-TOF mass spectrometry analysis of 17C peptide...

Ngày tải lên: 16/02/2014, 14:20