Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... bifunc- tional enzyme consisting of the RNase H domain and the acid phosphatase (APase) domain, and pro- pose that the enzyme is a novel style in the Type 1 RNase H family. Moreover, we also announce that S. ... protein also exhib- ited acid phosphatase activity at almost the same level as the C-terminal domain alone. These results indicate that...

Ngày tải lên: 19/02/2014, 18:20

... kinet- ics, and a half-life of inactivation of 330 min (5.5 h) was calculated (Fig. 7A and insert). This shows that McoP is a robust catalyst, although to a lower extent than McoA from A. aeolicus [5] and ... in par- ticular, are promising for industrial applications, as they have high intrinsic thermal and chemical stability. The search for MCOs, among the genomes...

Ngày tải lên: 18/02/2014, 04:20

... relevant candidates earli- er. Statistical and semantic features can comple- ment each other. Therefore, we can gain better performance if we consider them together. The contributions of this paper ... related candidate words earlier. Alterna- tively, we can also set a threshold for Score(w,c), which keeps the candidate word c with Score(w,c) larger than the threshold. We argue...

Ngày tải lên: 20/02/2014, 04:20

... that they can be easily adapted to other languages. 178 2.4.1 Explicit Feature Extraction We generate bi-grams in character level for every feature in the specification tree, and then match them ... specifications as input, the clustering methods can collect several hundred features. Then we r un our algorithm on the data and eval- uate the precision and recall. We also run the...

Ngày tải lên: 20/02/2014, 09:20

... three phytases (his- tidine acid phosphatase (HAP), cysteine phytase and purple acid phosphatase) by having a neutral (pH 7.0) rather than acidic pH optimum. Previous studies have shown that BPP ... compilation ª 2011 FEBS which suggests that PhyH-DI has the capacity to increase the catalytic efficiency of PhyH-DII. Further- more, the catalytic efficiency of PhyH was much...

Ngày tải lên: 14/02/2014, 15:20

... RT-PCR. The ACTIN2 gene was used as an internal standard. The results are expressed as fold induction compared with the time point 0 of untreated plants. Each measure- ment is the mean and standard ... [c- 32 P]-ATP. MBP was used as an artifi- cial substrate to assess the kinase activity and GST alone was used as a negative control. The top panel shows the kinase as...

Ngày tải lên: 14/02/2014, 19:20

... Cyclins B1 and B2 appear in S phase and accumulate in G 2 and mitosis before disappearing at the transition from metaphase to anaphase. Synthe- sis is controlled at the level of gene transcription ... on the CDE ⁄ CHR, the CHR region and the element upstream from it were replaced in the human cyclin B2 promoter with the well-characterized CDE ⁄ CHR sites from...

Ngày tải lên: 16/02/2014, 09:20

... reagents and the recombinant human TRAIL ⁄ APO 2 ligand were purchased from Invitrogen and Feldan Bio (St-Laurent, QC, Canada), respectively. The caspase 3 substrate (Ac-DEVD-pNA) and the inhibitor substrate ... [16,17]. The BH3 domain- only proteins are a subfamily of the Bcl-2 family involved in the initiation of apoptosis through the mitochondrial pathway. The...

Ngày tải lên: 16/02/2014, 09:20

... that the pathway of mammalian ARE-mediated mRNA decay can be flexible. Recently, an excellent database compiling ARE containing transcripts was established [46] and it has been predicted that approximately ... demonstrated that human PAI-2 mRNA was inherently unstable, with a half-life of  1 h and that most of the destabilizing activity was attributed to the 3¢ UTR [24]...

Ngày tải lên: 16/02/2014, 09:20

... anti-flag WB anti-HA TIAR-flag BOIP-flag HA-hnRNP M RNaseA WB anti-flag WB anti-HA HA TIAR Merged DAPI TIAR-flag BOIP-flag HA-DDX21 RNaseA WB anti-flag WB anti-HA B HA-ASF/SF2 HA-p68 HA-hnRNP M HA-DDX21 Fig. ... with haemagglutinin (HA)-tagged candidates in 293T cells and Flag-IP products were analyzed by western blot analysis with anti-Flag and anti-HA sera. The specific- ity of the...

Ngày tải lên: 16/02/2014, 15:20