Tài liệu Báo cáo khoa học: The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis ppt

... The lipid ⁄ protein interface as xenobiotic target site Kinetic analysis of tadpole narcosis Joachim Altschuh 1 , Sebastian Walcher 2 and Heinrich Sandermann, Jr 3,4 1 Institute of Biomathematics ... many of the findings supporting lipid or protein target sites can also be interpreted in terms of the lipid ⁄ protein interface as a third...

Ngày tải lên: 19/02/2014, 18:20

... kinases, of which 23 are classified to the AGC VIII group [8,9]. The AGC kinases were named on the basis of their homology to the mammalian cAMP-dependent protein kinase A, cGMP-dependent protein kinase ... GST alone was used as a negative control. One tenth of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay....

Ngày tải lên: 14/02/2014, 19:20

... independent proteins and peptides leads to the disassembly of the spherical capsid inside the virion. Some of the folded domains of Gag are then able to reassemble as inde- pendent proteins. The matrix protein ... studies regarding the diverse CA–CA interactions and inter- faces involved either in the assembly of the immature HIV-1 capsid, or in the reassembly o...

Ngày tải lên: 18/02/2014, 06:20

... into the Gag and Pol polypeptides by the viral protease. Further cleavage of the Pol chain yields the viral integrase (p31), a new protease (p10), the reverse transcriptase (p50) and RNase H ... in a change of the buried surface at the dimeric interface when compared with the buried interface in the dimeric CTD. The affinity constant of CAI for CTD was measured by...

Ngày tải lên: 18/02/2014, 06:20

... destruction of the cartilage. The onset of this disease might be triggered by multiple factors such as mechanical overload, defects in the composi- tion of the ECM, or altered expression of transcription factors ... in the growth plate, and in chondrocytes of the joints [36]. The AP-2a knockout mouse died at birth, with severe malformations of the craniofacial sk...

Ngày tải lên: 18/02/2014, 08:20

... required for the insertion of KdpD-N To test whether the translocation of the periplasmic loops requires the pmf, the location of the loops was analysed in the presence of CCCP. As shown in Fig. ... recognizes the C-terminal cytoplasmic domain of KdpD. Therefore, if the periplasmic loop is cleaved by the protease, only a small shift of the molecular mass o...

Ngày tải lên: 20/02/2014, 11:20

... extract the first (base) noun phrase after the first “is”, “was”, “are”, or “were” in the first sentence of a Wikipedia article. The last word in the noun phase is then extracted and becomes the hyper- nym ... as the unit to alle- viate the effect of segmentation errors in morpholog- ical analysis and we also used their character-based method. The NER task is then treat...

Ngày tải lên: 20/02/2014, 09:20

... Coo- massie Brilliant Blue R250. Protein concentration was determined using the Bradford assay with BSA as the standard [28]. Phytase activity assay Phytase activity was determined by measuring the ... InsP 6 was identified on the basis of its 16S rDNA gene sequence, and was subjected to further experimentation. Cloning, sequence and structure determination of the phytase gen...

Ngày tải lên: 14/02/2014, 15:20

... evidence that the CDE ⁄ CHR in G 2 ⁄ M cells is devoid of proteins, but DREAM compo- nents were detected at CDE ⁄ CHR promoters in G 0 ⁄ G 1 as well as in S ⁄ G 2 ⁄ M phases, it is likely that the complex ... interpretation. The shift in exact position of the CDE would yield a spacer essential to define these elements as a CDE ⁄ CHR tan- dem site...

Ngày tải lên: 16/02/2014, 09:20

... EGF as above in the presence of 20 l M SP600125 or vehicle (dimethylsulfoxide). Cell survival was assessed using the MTT assay. Caspase 3 activity was assessed by measuring degradation of the ... cytochrome c release [22,23]. The subsequent release of caspase-activating factors strongly amplifies caspase 3 activation through the cleavage of its precursor, the pro-caspase...

Ngày tải lên: 16/02/2014, 09:20