Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx

... 2275 Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase Claire M. L. Barrett and Colin RobinsonDepartment of Biological ... expressing tatABC operons carrying combinations of these mutations in the TM spans of both TatA and TatB, namely (TatA ⁄3Gly + TatB ⁄ 3Gly) (TatA ⁄ 3Gly + TatB ⁄ V12P) (TatA ⁄I12P + TatB ⁄ 3Gly) and ... analyzed TatABC levels in cells expressing the various mutated subunits, and the data for the TatA and TatB mutants (expressed using the pBADvector) are shown in Fig. 1. The expression of the wild-type...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... on the right side of Fig. 3 are the deconvoluted spectra showing the mass and identity of the species detected. The deconvoluted spectra on the right side of Fig. 3 clearly show the formation of ... siteswithin the two domains. This was followed by coordi-nation of the fourth and ﬁfth metal ions to the adomain for completion of the metallation of thisdomain prior to ﬁlling of the b domain. ... thoroughlyevacuated and Ar-saturated to remove the bulk of the oxy-gen from the solutions, in order to prevent oxidation of the metal-free protein upon raising of the pH for the metalla-tion...

Tài liệu Báo cáo khoa học: Evidence for proteasome dysfunction in cytotoxicity mediated by anti-Ras intracellular antibodies pdf

... and 3) and anti-ubiquitin IgG (lanes 4, 5 and 6). The insoluble pool was revealed with anti-myc IgG (lanes 7, 8 and 9). The migration of molecular mass markers (in kDa) and of the heavy (c) and ... lanes 3 and 6). Moreover, analysis of the insoluble pool conﬁrmed the accumulation of the single-chain aD11-sec as a ladder of higher-electrophoretic-mobility bands (lane 9). Together these results ... cells and the vastmajority of anti-(Ras 6)-expressing cells were healthy and showed diffuse staining typical of soluble proteins (panel a and the cell shown in the right of panel g). These resultssuggest...

Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf

... required for the formation of the complex looping structure in the case of BX-C,while a shorter time is sufﬁcient for the establishment of the much simpler regulatory interactions of the his-tone ... elements of the putative upstream tethering region, grad-ual removal of which shifts the ratio between the strength (indica-ted by the thickness of the curved arrows) of the cis and trans interactions ... onecopy of them is present in a transgenic insert, and the signiﬁcance of this high afﬁnity pairing in the regula-tion of the Abd-B is therefore unclear. Perhaps tighthomologous pairing between these...

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... explain the dimerization of the bc1complex atthis stage (i.e. just after the addition of ISP and Qcr10p).On the other hand, in the transition from the 500 kDaband to the 670 kDa band, a structural ... bc1sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) ﬁnally occurs. These ﬁnd-ings ... describedpreviously [49,50]. Standard procedures were used for the preparation and ligation of DNA fragments, for the trans-formation of Escherichia coli and for the isolation of plas-mid DNA from bacterial...

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

... b4 and b8 of the (b ⁄ a)8barrel domain, and for rBAT also with the short stretch near the C-terminus of domain B [9,19]. From the sequence–structure point of view, the basic difference between ... the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH13 enzymes, and especially the sequence of ... iden-tiﬁable, even for the enzymatically active GH13 mem-bers [13]. Therefore, one of the goals was to aligncorrectly the b5-strands of the hcHAT sequences,which was especially problematic for the 4F2hc...

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... possibilities for the actual mechanism by which the virus is able to travel from the cell membrane to the nucleus. A similar effectwas observed in CFs and keratinocytes [29,30]. A pHdependence for the formation ... (0,24, 36, 48 and 60 h), the cells were ﬁxed by using ﬁxativebuffer (2% formaldehyde and 0.2% glutaradehyde) at roomtemperature for 20 min, and then stained with Giemsa for 45 min. The cells were ... as the control. For fusion, at 18 h post-transfection, the target and the effector cells were mixed together (1 : 1 ratio) and cocultivated in 24-well dishes. The activation of the reporter luciferase...

Tài liệu Báo cáo khoa học: Progress for dengue virus diseases Towards the NS2B–NS3pro inhibition for a therapeutic-based approach ppt

... chemother-apy regimes for the prevention and treatment of DF and DHF, the understanding and the biochemicalcharacterization of the life cycle of DENv have madesubstantial progress over the ... such as the resolution of the structure of its complex with an inhibitor.On the other hand, the NTPase ⁄ helicase region of the NS3 protein, and the surface of NS3 protein withProgress for dengue ... region 40–66 of the cofactor is important in the folding of the protein and that the C-terminal part of the cofactor,which is absent in the truncated form, directly interactswith the substrate-binding...

Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc

... with the protein activity. The tagged protein was puriﬁed and then used for furtheranalysis.In the reductive reaction, the Michaelis–Menten con-stant Km for DHA was 1 mm, and the Km for NADPH ... genomic DNA. For that rea-son, we ampliﬁed the open reading frame from cDNA. For the second of the two potential glycerol dehydro-genases, we predicted no introns and therefore ampli-ﬁed the open ... glycerol and NADP toform d-glyceraldehyde and NADPH; the other is aglycerol:NADP+2-oxidoreductase (EC 1.1.1.156) thatfacilitates the reaction of glycerol and NADP to formDHA and NADPH. The enzymes...

Tài liệu Báo cáo khoa học: "Sentence-For-Sentence Translation: An Example" ppt

... selected from an infinite subset of the set of all Arabic sentences and submitted to the computer for translation. Before the production of the sets of orders for the construction of the output ... The selection of the sub- subscripts IGNORANT and CHILD for JAHLH and JAHL, re- spectively, and of the subsubscripts BEAUTIFUL for JMYLH and HANDSOME for JMYL illustrates the capacity of ... side of the allograph MN of the verb stem MNN. The analysis of the two interpretations has reached the level of the dotted lines in the double analysis in Figure 3. The allograph MN of the...

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