Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx

... 2275 Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase Claire M. L. Barrett and Colin Robinson Department of Biological ... expressing tatABC operons carrying combinations of these mutations in the TM spans of both TatA and TatB, namely (TatA ⁄ 3Gly + TatB...

Ngày tải lên: 19/02/2014, 17:20

... on the right side of Fig. 3 are the deconvoluted spectra showing the mass and identity of the species detected. The deconvoluted spectra on the right side of Fig. 3 clearly show the formation of ... sites within the two domains. This was followed by coordi- nation of the fourth and fifth metal ions to the a domain for completion of the metallation of...

Ngày tải lên: 19/02/2014, 00:20

... and 3) and anti-ubiquitin IgG (lanes 4, 5 and 6). The insoluble pool was revealed with anti-myc IgG (lanes 7, 8 and 9). The migration of molecular mass markers (in kDa) and of the heavy (c) and ... lanes 3 and 6). Moreover, analysis of the insoluble pool confirmed the accumulation of the single- chain aD11-sec as a ladder of higher-electrophoretic- mobility...

Ngày tải lên: 21/02/2014, 00:20

... required for the formation of the complex looping structure in the case of BX-C, while a shorter time is sufficient for the establishment of the much simpler regulatory interactions of the his- tone ... elements of the putative upstream tethering region, grad- ual removal of which shifts the ratio between the strength (indica- ted by the thickness of...

Ngày tải lên: 20/02/2014, 01:20

... explain the dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural ... bc 1 sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of...

Ngày tải lên: 18/02/2014, 08:20

... b4 and b8 of the (b ⁄ a) 8 barrel domain, and for rBAT also with the short stretch near the C-terminus of domain B [9,19]. From the sequence–structure point of view, the basic difference between ... the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs a...

Ngày tải lên: 18/02/2014, 13:20

... possibilities for the actual mechanism by which the virus is able to travel from the cell membrane to the nucleus. A similar effect was observed in CFs and keratinocytes [29,30]. A pH dependence for the formation ... (0, 24, 36, 48 and 60 h), the cells were fixed by using fixative buffer (2% formaldehyde and 0.2% glutaradehyde) at room temperature for 20 min, and the...

Ngày tải lên: 18/02/2014, 14:20

... chemother- apy regimes for the prevention and treatment of DF and DHF, the understanding and the biochemical characterization of the life cycle of DENv have made substantial progress over the ... such as the resolution of the structure of its complex with an inhibitor. On the other hand, the NTPase ⁄ helicase region of the NS3 protein, and the surfa...

Ngày tải lên: 19/02/2014, 00:20

... with the protein activity. The tagged protein was purified and then used for further analysis. In the reductive reaction, the Michaelis–Menten con- stant K m for DHA was 1 mm, and the K m for NADPH ... genomic DNA. For that rea- son, we amplified the open reading frame from cDNA. For the second of the two potential glycerol dehydro- genases, we predicted no int...

Ngày tải lên: 19/02/2014, 06:20

... selected from an infinite subset of the set of all Arabic sentences and submitted to the computer for translation. Before the production of the sets of orders for the construction of the output ... The selection of the sub- subscripts IGNORANT and CHILD for JAHLH and JAHL, re- spectively, and of the subsubscripts BEAUTIFUL for JMYLH...

Ngày tải lên: 19/02/2014, 19:20