Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt

... mainly associated with the plasma membrane or with the membranes of intracellular organelles although they lack a transmembrane domain. They are attached to the cytoplasmic face of the membranes via ... G G Context ATG 1 330 AAG ATG A Adequate ATG 2 345 CTG ATG T Weak ATG 3 396 CCC ATG A Weak ATG 4 489 CTG ATG A Weak Hu-K4 A. Munck et al. 1 722 FEBS Journal 27 2...

Ngày tải lên: 19/02/2014, 17:20

... from the periplasmic fraction to near homogeneity 22 20 18 16 12 14 10 8 6 4 2 0 0 5 10 20 25 15 22 20 18 16 12 14 10 8 6 4 2 0 22 20 18 16 12 14 10 8 6 4 2 0 20 18 16 12 14 10 8 6 4 2 0 2. 2 2 1.8 1.4 1 .2 1.6 1 0.8 0.6 0.4 0 .2 0 2 1.8 1.4 1 .2 1.6 1 0.8 0.6 0.4 0 .2 0 2. 2 1 .2 1.7 0.7 0 .2 –0.3 2. 2 1 .2 1.7 0.7 0 .2 –0.3 GB17 ... (h) 0 0 5 5...

Ngày tải lên: 15/02/2014, 01:20

... antibiotics. Post-translational regulation was discussed by Aaron Ciechanover (Haifa, Israel), who argued that the ubiquitin proteolytic system covers the pathway for elucidating the basic mechanisms that are pivotal ... gold-labeled substrates. Nuclear export emerges as the major regulatory mechanism of the nuclear accumulation of STAT2, as H. Soreq and A. Honigman It is all abo...

Ngày tải lên: 19/02/2014, 02:20

... (Table 2) . This is at first sight a puzzling result, as hMS was isolated in the inactive form with the co- factor in the Co 3+ oxidation state (i.e. AqCbl). Activity may arise from: (a) a small amount ... interactions between the MSR FMN domain and MS. Abbreviations AD, activation domain; AqCbl, aquacob(III)alamin; ATR, ATP:cobalamin adenosyltransferase; CPR, cytochrome P450...

Ngày tải lên: 18/02/2014, 08:20

... pulchellin A- chains, the residues involved in the active site cleft are the same as in abrin and ricin A- chains. This suggests that the catalytic reaction is exactly the same. The sugar binding ... CTC GAG TTA AAA CAA ATG AAG-3¢), pul- cintFW1 (5¢-CCT GTG CTT CGA GAT CCA AC-3¢), pulcintFW2 (5¢-GCA TCT ACC TAC CTT TTC AC-3¢), pulcintRW1 (5¢-CAC CCA TCG TTG GCT AGC CC-3¢) and...

Ngày tải lên: 18/02/2014, 16:20

... 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAATC GTCCC-3¢; NP5, 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) ... upstream primer (OP17: 5¢-GA AAAACCATGGTGAATGAATACCAAGCGACT-3¢ ) and downstream primer (NP7: 5¢-CAATCTCCATGGCTAG TAGCTTGCACTCAG-3¢) containing a NcoI restric...

Ngày tải lên: 19/02/2014, 07:20

... chitosan by pronase and characterization of the resultant products Acharya B. Vishu Kumar 1 , Lalitha R. Gowda 2 and Rudrapatnam N. Tharanathan 1 1 Department of Biochemistry and Nutrition, 2 Department ... 1513–1518. 41. Watanabe,T.,Kobori,K.,Kiyotaka,Miyashita,Fujii,T.,Sakai, H. & Makot, Uchida and Tanaka, H. (1993) Identification of glutamic acid 20 4 and aspartic acid 20 0 in c...

Ngày tải lên: 19/02/2014, 12:20

... Arg506 is % 20 -fold faster than cleavage at Arg306, the major part (% 95%) of Ó FEBS 20 04 Heparin and APC-catalyzed inactivation of FVa (Eur. J. Biochem. 27 1) 27 25 FVa is inactivated via pathway 2. ... cleavages at Arg306 and Arg506 appear to be crucial for inactivation, but the cleavage at Arg679 is probably less important [10]. The cleavage at Arg506 is kinetically fa...

Ngày tải lên: 19/02/2014, 13:20

... other two other thiol–disulfide exchange enzymes, Escherichia coli DsbA and DsbC, as well as the isolated catalytic a domain of PDI. Both the PDI a domain and DsbA have a catalytic site, with an ... Journal 27 7 (20 10) 24 54 24 62 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 24 57 To study the potential mechanism of action of baci- tracin, the insulin reduction...

Ngày tải lên: 16/02/2014, 14:20

... pathway). Bottom: cleavage to yield AMPA and glyoxylate (the AMPA pathway), referred to as the GOX pathway. (B) Reaction catalyzed by GO on glyphosate, an alternative to the AMPA pathway as catalyzed ... non-target-site mechanisms might be the major causes of most glyphosate-resistant biotypes. In the case of Conyza canadensis, glyphosate accumulates in vacuoles of resis- tant plants...

Ngày tải lên: 14/02/2014, 14:20