Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx

... k lim D (s )1 ) K d D (lM)KIE 5.0 0.36 ± 0. 01 3 81. 9 ± 24 0 .13 ± 0. 01 418 .2 ± 17 2.8 ± 0.29 5.5 0.56 ± 0. 01 228.2 ± 8.5 0 .18 ± 0. 01 263.9 ± 14 3 .1 ± 0.23 6.0 0.92 ± 0. 01 118 .1 ± 4.0 0.26 ± 0. 01 118 .3 ... than the corresponding turnover number of % 1. 2 s )1 (with 1 and 5 mm PES), suggesting that the chemistry of the oxidative half-reaction and bindi...

Ngày tải lên: 19/02/2014, 07:20

... Thus, the theoretically calculated extinction coefficient of IF–CblOH 2 in the UV-partofthespectrumwase 280 ¼ 59 400Æ M )1 Æcm )1 according to e IF 280 ¼ 40 300Æ M )1 Æcm )1 of the protein moi- ety ... overlapping absorbance of CblOH 2 e Cbl 280 ¼ 19 10 0Æ M )1 Æcm )1 at pH 7.5 (IF:Cbl ¼ 1: 1).If we conjecture that, for example, 30% of IF in the preparation i...

Ngày tải lên: 21/02/2014, 00:20

... of our art? Instead of assuming that the problem is one of using typed English to access and/ or update a file or files in a single database system, let us define a spectrum of potential natural ... officers represented in it. The system merely made no distinction between "all" and "any", and interpreted the final query to mean the But, you...

Ngày tải lên: 21/02/2014, 20:20

... transactivation domain. The DNA-bind- ing domain is responsible for DNA binding specificity and dimerization, and the ligand-binding domain is responsible for binding of the ligand and associated induced ... Canaani E (19 98) The C -terminal SET domains of ALL -1 and TRITHORAX interact with the INI1 and SNR1 proteins, components of the SWI ⁄ SNF complex. Proc Natl...

Ngày tải lên: 16/02/2014, 14:20

... seen in the crystal structure of the con- ventional hairpin ribozyme [ 21, 31] . Variation in linker length and ⁄ or the length of the duplexes flanking the single-stranded linker will, therefore, in uence ... Observation of internal cleavage and ligation reactions of a ribozyme. Nat Struct Mol Biol 11 , 11 07 11 13. 24 Nesbitt SM, Erlacher HA & Fedor MJ (19 99...

Ngày tải lên: 20/02/2014, 01:20

... way both new information nuggets and validated back- ground information nuggets interactively. Fig. 1 summarizes the main components and the informa- tion flow. Figure 1: Blueprint of the proposed ... pages that contain the information the user is interested in. The following aspects are important in this context: 1) Users basi- cally have to know what they are looking...

Ngày tải lên: 20/02/2014, 05:20

... in obese mice In order to gain insights into the potential biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in ... literature [15 ], genes of the miR -17 ⁄ 92 cluster, including miR -17 -5p, miR-20, and miR-92, were upreg- ulated during differentiation (Fig. 1A, right panel). We fur...

Ngày tải lên: 18/02/2014, 08:20

... to the surface of SVT2 cells and is internalized inside the cells, whilst the MCM monomer does not bind and is not internalized [11 ]. We repeated these experiments with the MSSAE monomer and treated ... were then treated with MSSAE, then stripped of surface bound proteins with a high salt solution made up of 1 M Hepes pH 7.5 containing 0 .1 M NaCl [11 ], and permeabi...

Ngày tải lên: 20/02/2014, 11:20

... is not possible to infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of defensins. However, our ... obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutational analysis [35] and st...

Ngày tải lên: 20/02/2014, 11:20

... distance of 3.5 A ˚ , and the shortest distance between the imidazole ring of His 210 and the atoms of Asp 113 is 3.2 A ˚ . The substrate binding subsite in API is composed of His 210 -Gly 211 -Gly 212 , ... Ser 214 –Trp 215 –Gly 216 and His 210 –Gly 211 –Gly 212 , respectively. S1 pocket is the substrate binding site for the side- chain of Lys (API) or Lys and...

Ngày tải lên: 21/02/2014, 03:20