Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt

... dimerization of cystatin F could be a process regulating its inhibitory properties. Screening of proteases for their inhibition showed that cystatin F is different from other cystatins, both in terms of ... weight stand- ards. (B) Inhibitory activity of cystatin F against papain. Cystatin F (500 n M) was incubated 15 min at 37 °C in phosphate buffer pH 6.0...

Ngày tải lên: 19/02/2014, 17:20

... thank Prof. Steve C. Fry, Institute of Cell and Molecular Biology, Univer- sity of Edinburgh, Edinburgh, UK, and Dr Albert Schulte, Ruhr University of Bochum, Germany, for critical reading of the ... chitooligosaccharides, and chitin. The ini- tial velocity of the enzyme for concentrations of the substrates ranging from 0 to 2.0 mm was determined after 5 min of reaction. G...

Ngày tải lên: 20/02/2014, 01:20

... Schematic representation of the different Mxi1 isoforms tested for suppres- sive potential in the REF transformation assay. All of the isoforms carried Myc tags on their COOH termini. SID, Sin3 interacting domain; ... absence of doxycycline. (B) Silver staining of an anti-Flag IP per- formed on lysates from HeLa Tet ON cells expressing either empty vector, FLAG-Mxi1-SRa or FLAG-Mx...

Ngày tải lên: 18/02/2014, 16:20

... v) fetal bovine serum (Biochrom KG, Berlin, Ger- many). Paired cultures of cells growing in normal media or in iron-rich media were set up and maintained for different periods of time as indicated. ... the other hand, a recent in vitro study examining the effect of iron loading on gene expression in HepG2 cells by differential display revealed that iron can affect mRNA lev...

Ngày tải lên: 19/02/2014, 16:20

... available: Fig. S1. Alignment of the long loop region in the calf- 2 module of integrin’s a subunits. Fig. S2. A gallery of structures predicted by Robetta [20] for the long loop in the calf-2 region of ... shown in Fig. 1. Apart from studies demonstrating the necessity of this cleavage for the proper function of a v b 5 [47], little is known of its role in integrins l...

Ngày tải lên: 18/02/2014, 04:20

... Membrane binding properties of K-Ras. (A) Membrane fractions of CHO-K1 cells expressing YFP-K-Ras C14 or YFP-K-Ras full or CFP-H- Ras C20 were obtained as described in Fig. 1B and then incubated for ... and cytosolic fractions from nontransfected cells or cells expressing YFP, YFP-K-Ras C14 or YFP-K-Ras full were obtained as described in Fig. 1. Membrane fractions of tra...

Ngày tải lên: 19/02/2014, 00:20

... amino acid residues of the heparin-binding site (HBS) of VVA2 (166–194). (B) Inhibitory effects of VVA1 on the binding of VVA2 to liposomes. After incubation of VVA1, VVA2 or the mixture of VVA2 ... to bind to the membrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were...

Ngày tải lên: 19/02/2014, 06:20

... lanes 2, 4 and 6 in Fig. 1A but the use of a higher exposure allows the effects of the mutants to be more evident. Again shifted Myb-bands were observed in addition to the main 75 kDa band (Fig. 1B, ... expressing lamin (pLam) and full-length human c-Myb (pDBT-hcM-FL) were transformed into the a-mating type of the same strain. Mating was performed to create the diploid combinati...

Ngày tải lên: 21/02/2014, 00:20

... shown). Role of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. The first approach involved measuring the level of ... mitochondrial ferredoxin (FDX) + ferredoxin reductase (FDXR), homologs of mammalian ferredoxin and ferrodoxin reductase [27,28]. Results Expression of intact and trun...

Ngày tải lên: 18/02/2014, 16:20

... of folic acid, i.e. the small intestine. Expression of the RFC protein in BBM of the intestine The effect of chronic alcoholism on the level of expres- sion of the RFC protein at the BBM surface ... sec/mg protein) Fig. 2. [ 3 H]Folic acid transport in intestinal BBMVs at different intervals during the course of ethanol administration. An incubation buffer of pH 5.5 and...

Ngày tải lên: 18/02/2014, 16:20