Tài liệu Báo cáo khoa học: An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding docx

... FEBS An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding Peter Du ¨ rrschmidt*, Johanna Mansfeld and Renate Ulbrich-Hofmann Department of ... Model of the 3D structure of the neutral protease from Bacillus stearothermo- philus, including the position of the disulfide bridge in mut...

Ngày tải lên: 19/02/2014, 17:20

... nutrient sensing in the brain and is an example of an enzyme that can sense and respond to the nutritional environment. The major challenge to understanding the role of CPT1c is to determine its ... during periods of an abundant food supply to hedge against periods of food shortage. Today, humans have pushed storage too far, to the point of widespread obe...

Ngày tải lên: 14/02/2014, 22:20

... on the 2009 elec- tion of the Portuguese Parliament. These took place between the 2 nd and the 12 th of September, 2009, and involved the candidates from the largest Portuguese parties. The ... Sentiment Analysis”. In Proc. of the Joint Hu- man Language Technology Conference and Empiri- cal Methods in Natural Language Processing, Canada. 568 Proceedings of the...

Ngày tải lên: 20/02/2014, 05:20

... mathematically better defined than through other methods, because it enables one to develop a broader perspective of the questions within an analysis of the hypothesis in the con- text of ... can occur and affect the state of the process. The most important aspect of the control is that all of the serial order computations can occur simultaneously and af...

Ngày tải lên: 21/02/2014, 20:20

... deep knowledge of the installation and maintenance of the tool, thus allowing all the other users to benefit 1 6 Evaluation The evaluation of the factory is based on its features and usability ... monitors the status of the WSs deployed and offers multi- ple metadata fields to annotate WSs. 3.3 Workflows: Taverna Now that users can find WSs and use them, the next step...

Ngày tải lên: 22/02/2014, 03:20

... (ortho- logue of human RPE65) and retains all of the known key residues for the enzymatic activity of RPE65. Similar to the other RPE-specific RPE65, RPE65c was present in both the membrane and cytosolic ... were used to identify the subcellular localization of RPE65c and to verify the endoplasmic reticulum membrane preparation. The distribution of RPE65c in each...

Ngày tải lên: 14/02/2014, 14:20

... Lethal factor (LF) is the proteolytic component of the toxin, and cleaves six members of the mitogen-activated protein kinase kinase family after being delivered to the cytosol by the cell- binding ... 2009 The Authors Journal compilation ª 2009 FEBS pro-IL-1b in the cytosol and the appearance of IL-1b in the cell supernatants (Fig. 2C). A lower level of IL-1b was...

Ngày tải lên: 16/02/2014, 09:20

... two of them, the L3-A and YIL-A mutants, whereas the others showed similar activity in this assay as the wild type (Fig. 6C). We next tested the effect of these mutants on activation of the Pbx–Meis ... activity of a linked AD To further delineate the region required for the inhibi- tory effect of the Hth domain, we created a series of GDB fusion proteins (Fi...

Ngày tải lên: 16/02/2014, 15:20

... conserved. A12 can be changed to T12, and A15 can be changed to G15. When 8–17 DNAzyme binds its substrate, a gÆT wobble pair can be formed, and is considered to be significant and crucial for the catalytic ... dramati- cally decreased the rate of DNA self-cleavage. We systematically varied the length of the RS to determine the effects on regulation of the DNA self-cl...

Ngày tải lên: 16/02/2014, 15:20

... to affinity chromatogra- phy. Aliquots of the input and the eluate of the chromatography were resolved by Tricine ⁄ SDS ⁄ PAGE and then analyzed by Coo- massie Brilliant Blue staining (left) and ... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig...

Ngày tải lên: 18/02/2014, 13:20