Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio rerio) doc

... mammalian origin by the fish intestine. Comparison of zebrafish prp1, prp2, and prp3 developmental expression patterns The developmental expression patterns of prp1 and prp2 coding for long zebrafish ... 2004 FEBS 513 Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebrafish (Danio...

Ngày tải lên: 19/02/2014, 16:20

... proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins reduce the expression of mRNA targets by binding in 3¢ UTR regulatory elements, thus controlling translation ... cis-acting elements usually located in the 3¢ UTR. In recent years, and as a result of great efforts aiming to understand the mechanism of RNA control in animals, the f...

Ngày tải lên: 18/02/2014, 06:20

... isoform. Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E. coli For protein expression in E. coli, only the cDNA coding for the mature proteins ... participating in an IgE-binding epitope. The N-terminal sequencing of a 52-kDa glyco- protein of tomato extract gave hints for the existence of b-fructofuranosidase as a rel...

Ngày tải lên: 21/02/2014, 00:20

... but are often generic and mark masculine, feminine, or plural (Gross, 1977; Goffic and McBride, 1975). And furthermore, these same articles often are not translated into meaning preserving sentences ... The study includes off-line picture naming which forces use of articles in all of the above contexts, as well as on-line production of these morphemes in an attempt to d...

Ngày tải lên: 21/02/2014, 20:20

... processing), and GmPDIL-1 and GmPDIL-2 associate with proglycinin and b-con- glycinin in the ER, suggesting that they may play important roles in folding and in formation and rearrangement of disulfide ... synthesis levels of both b-conglycinin and glycinin, as pro-b-conglycinin and proglycinin are transient pro- tein forms that are present in the ER prior to process-...

Ngày tải lên: 18/02/2014, 11:20

... and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and ... connects the a5 and a6 helices in the pRN1 prim–pol protein. Because of a two-residue gap in the corresponding region of the Rep245 sequence, this finding had not been pred...

Ngày tải lên: 18/02/2014, 18:20

... its role in the synthesis of L -cysteine and L -glycine and also in the formation of L -methionine by the interconversion of L -cysteine via L -cystathionine, L -serineisamajorprecursorofphosphat- idyl- L -serine, ... of the nucleotide binding domain of one of the adjacent subunits. Site directed mutagenesis of Trp139 to Gly resulted in the dissociation of the tetram...

Ngày tải lên: 19/02/2014, 13:20

... presence of three distinct domains: the rigid CORE-domain and two smaller peripheral domains or mobile parts, the NMP-binding site and the LID-domain [4]. The NMP-binding site has many intermolecular contacts ... were obtained by simple rotation of the same model. The flexible LID domain appears at top of the three figures, NMP-binding site and ATP binding domain are displayed, NH 2...

Ngày tải lên: 21/02/2014, 00:20

... accompanied by improved bind- ing to longer ligands (up to five atoms in the main chain) and retained binding to shorter ligands [55]. Of the 21 changes in the evolution of R7 from native GLYAT ... thermo- stability and protein expression in E. coli determined for wild-type GO and variants of GO obtained by site-saturation mutagenesis of the positions identified by docking...

Ngày tải lên: 14/02/2014, 14:20

... leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation of unique branched-chain amino acid catabolism in insects. Abbreviations ACAD, ... the same molecular size and expression levels (Fig. 2A). Determination of full-length cDNA sequences using the RACE method in both strains revealed the...

Ngày tải lên: 18/02/2014, 04:20