Tài liệu Báo cáo khoa học: Various secretory phospholipase A2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells docx

... (2005) 655–672 ª 2005 FEBS Various secretory phospholipase A 2 enzymes are expressed in rheumatoid arthritis and augment prostaglandin production in cultured synovial cells Seiko Masuda 1 , Makoto ... distributed in various cells, such as synovial lining and sublining cells and vascular cells, as well as in extracellular matrix fibers [46]. I...

Ngày tải lên: 19/02/2014, 16:20

... the kinetic bind- ing data describing the sum of these kinetic events could not be adequately simulated by simple binding models commonly used in the kinetic fitting of pro- tein–protein interaction ... containing over 20 grouped PS molecules). We have shown that doubling the PS concentration in the membrane results in an increase in the number of binding sites (compare Fig. 3D and 3...

Ngày tải lên: 18/02/2014, 06:20

... signalling proteins, and to the expression of pro- and in ammatory cytokines. There is evidence that the heteromeric guanine nucleotide- binding regulatory protein (G protein) is also involved in ... change. Ligands include hormones, biogenic amines, histamine, serotonin and lipid deri- vates, but also immunological and in ammatory medi- ators such as chemokines. Heterotrimeric G...

Ngày tải lên: 18/02/2014, 13:20

... the cells with BEL induced a significant accu- mulation of cells in G 2 ⁄ M and S, and a concomitant decrease of cells in G 1 , with respect to untreated cells. In contrast, pyrrophenone induced ... phospholipase A 2 in fatty acid incorporation, phospholipid remodeling, lysophosphatidylcholine generation, and secretagogue- induced arachidonic acid release in pancreatic...

Ngày tải lên: 18/02/2014, 17:20

... validated in vitro andusedtoexamine the branch-point kinetics in detail and to obtain insights into the kinetic controls of methionine and threonine synthesis in plants. The branch-point between ... methionine and threonine biosynthesis pathways in Arabidopsis the following procedure was used. Firstly, the kinetic links inside the branch-point and between the branch-point syst...

Ngày tải lên: 20/02/2014, 02:21

... collagens, gelatin, and laminin, and also non-ECM proteins, including pro-tumor necrosis factor-a and a 2 -macro- globulin [2]. MMP-9 (gelatinase B) is secreted by neu- trophils and macrophages and has ... 150 mm; Dionex) and elution using a linear binary gradient: 5–65% ACN in 0.1% formic acid in 35 min, maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min. The typi...

Ngày tải lên: 16/02/2014, 14:20

... point (+1) and the extended )10 and )35 are indicated (blue letters). The ribosome-binding site (RBS) and translation initiation codon (Met) of kis are underlined and shown in red. The N-terminal ... encoding a toxin and the other an anti- toxin, which are expressed at a low level. The toxin is neutralized in cells containing the plasmid by continu- ous production of...

Ngày tải lên: 18/02/2014, 04:20

... DBD–DBD interaction is important in the HSF–HSF and HSF–HSE interactions. In HSF DBD– HSE co-crystals, the protein–protein interface consists of the helix 2 amino-terminus, the turn and the wing [15]. ... DNA-binding domain (DBD) at the amino-terminus and the oligomerization domain in the central region of the protein [1,4]. The HSF DBD belongs to the ‘winged’ helix-turn-helix fam...

Ngày tải lên: 18/02/2014, 04:20

... miRNAs are single-stranded, nonprotein-coding RNA molecules, approximately 19–25 nucleotides in length. By binding to the complementary sites in the 3¢UTRs of their target genes, they can induce ... cell line and NIH3T3+ indicates the cells overexpressing miR-192 ⁄ 194. (C) Graph showing the periodicity of Bmal1 mRNA levels in NIH3T3 cells with high levels of miR-192 ⁄ 194 and...

Ngày tải lên: 18/02/2014, 06:20

... the binding of RNA-binding proteins to 3¢ UTR tran- scripts has been poorly described in plants. PUF proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins ... redundantly in sperm–oocyte switch and germ stem cell maintenance [7,15]. However, these two proteins show distinct patterning functions in the distal germ line, independently affecting...

Ngày tải lên: 18/02/2014, 06:20