Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

... translational inhibition [1]. In the cytoplasm of human cells, the dsRNA binding proteins HIV transactivating response RNA-binding protein (TRBP) and Dicer recognize and bind the siRNA and form RNA-induced ... highly active siRNAs. Results and discussion Design of siRNAs and EGFP silencing efficiency Designing siRNAs with an intentional bias in hybrid- ization stabili...

Ngày tải lên: 18/02/2014, 06:20

... intermediates, it is rapidly oxidized to sulfinic or sulfonic acid, and the enzyme is irreversibly inactivated [18]. Our examination of the sensitivity and reversibility of oxidation in PhyAsr indicates ... determined in the absence of a ligand in the active site. Least squares superposition of the P-loop main chain (starting at the residue prior to the cataly...

Ngày tải lên: 18/02/2014, 18:20

... hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus Hiroki Matsudo 1 , Kyoichi Osano 1 , Hiroshi Arakawa 2 and Masao Ono 1 1 ... observed in transient transfection assays. Absence of Ig-b mRNA in cells with a deletion in region II (Fig. 4A) demonstrated the e...

Ngày tải lên: 19/02/2014, 16:20

... for the visualization of the protein composition of the fractions. Ryanodine-binding Ryanodine-binding assays were carried out using [ 3 H]ry- anodine in a medium having the composition of 1 ... calcium-binding sites of rabbit sarcoplasmic reticulum. RyR1s were reconstituted into an artificial lipid bilayer and the effect of gadolinium was investigated from the cy...

Ngày tải lên: 19/02/2014, 16:20

... of AIDS, and various PR inhibi- tors are now in clinical use. However, there is a rapid selec- tion of viral variants bearing mutations in the proteinase that are resistant to clinical inhibitors. ... selected against saquinavir and amprenavir [18], together with Gag mutations against two BILA inhibitors [23], and in patients treated with ritonavir [22] or indinavir [24]. Ba...

Ngày tải lên: 21/02/2014, 03:20

... this distribution for the correct bilingual term pairs, which are contained in the topmost 200 TP(t E ) and are found in the existing bilingual lexicon (we have similar results for other sites ... evaluated sim- ply consulting a bilingual lexicon instead of employing an MT software. As reported in Collier and others (1998), the precision of simple word by word query t...

Ngày tải lên: 22/02/2014, 02:20

... After extensive washing with NaCl ⁄ Tris, PV-positive fibers were visualized by incubation of sections with 3,3¢ -diaminobenzidine (DAB) and analysis of immuno- stained sections. Cardiolipin measurements Total ... chronic exercise [8]. The mechanism of control of mitochondrial biogenesis in muscles in response to muscle activity, as well as the mechanism controlling different...

Ngày tải lên: 19/02/2014, 07:20

... preparation [28]. Discussion Protein identification is greatly facilitated by the growing number of genome sequences. To get further insight into the function of a protein, additional infor- mation ... N-terminus instead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intact protein at its a-amino group and represents the amino te...

Ngày tải lên: 19/02/2014, 18:20

... further assayed by flow cytometry with annexin V–FITC ⁄ pyridine iodination (PI) double staining, which identified apop- totic cells as annexin V positive, but PI negative. This method obtained ... Hsp70-medi- ated protection against H 2 O 2 -induced apoptosis is not clear. In the present investigation, we further studied the effects of nucleolin ⁄ C23 ablation on Hsp70-medi- ated pro...

Ngày tải lên: 16/02/2014, 09:20

... domain can inhibit a linked activation domain, and this inhibition is not limited to the Meis2 activation domain. Database searching reveals that the Meis3.2 splice variant, which is found in ... transcriptional activation domain (AD), the activity of which is inhibited by the Hth domain. This autoinhibition can be relieved, in part, by interaction with Pbx1, and maps to a r...

Ngày tải lên: 16/02/2014, 15:20