Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efﬁcacy pdf

... translational inhibition [1]. In the cytoplasm of human cells, the dsRNA bindingproteins HIV transactivating response RNA-bindingprotein (TRBP) and Dicer recognize and bind the siRNA and form RNA-induced ... highly active siRNAs.Results and discussionDesign of siRNAs and EGFP silencing efﬁciencyDesigning siRNAs with an intentional bias in hybrid-ization stability is intended to maximize correct guidestrand ... guidestrand selection and loading into the RISC. This isbeneﬁcial both in achieving strong silencing and alsominimizing off-target silencing by the passenger strand[45]. Thus the relative thermodynamic...

Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx

... intermediates, it is rapidly oxidizedto sulﬁnic or sulfonic acid, and the enzyme is irreversiblyinactivated [18]. Our examination of the sensitivity and reversibility of oxidation in PhyAsr indicates ... determined in the absence of a ligand in the active site. Least squaressuperposition of the P-loop main chain (starting at the residue prior to the catalytic cysteine and ending after the conserved ... PTP1B [12,13] and RPTPa [15], the posi-tions of the active site arginine and the WPD loop are in the open (general acid) conformation. The general acidloop and active site arginine in PhyAsr are...

Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx

... hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus Hiroki Matsudo1, Kyoichi Osano1, Hiroshi Arakawa2 and Masao Ono11 ... observed in transienttransfection assays. Absence of Ig-b mRNA in cellswith a deletion in region II (Fig. 4A) demonstrated the essential role of this region in the transcription of the Ig-b gene. Deletion ... transcription of the Bcell-speciﬁc Ig-b gene and in maintenance of active chromatin state in the Ig-b locus were examined. A total of 10 DHSs were divided into fourregions, and each region was...

Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx

... for the visualization of the protein composition of the fractions.Ryanodine-bindingRyanodine-binding assays were carried out using [3H]ry-anodine in a medium having the composition of 1 ... calcium-binding sites of rabbit sarcoplasmic reticulum.RyR1s were reconstituted into an artiﬁcial lipidbilayer and the effect of gadolinium was investigatedfrom the cytoplasmic and the luminal side ... reversible and concentration-dependent manner causing the inhibition of the channel with similar Hill coefﬁcient and IC50values. The cis-binding sites seem not to be identicalwith the known cis...

Tài liệu Báo cáo khoa học: Effect of sequence polymorphism and drug resistance on two HIV-1 Gag processing sites pdf

... of AIDS, and various PR inhibi-tors are now in clinical use. However, there is a rapid selec-tion of viral variants bearing mutations in the proteinase thatare resistant to clinical inhibitors. ... selectedagainst saquinavir and amprenavir [18], together with Gagmutations against two BILA inhibitors [23], and in patientstreated with ritonavir [22] or indinavir [24]. Based on clinicalstudies, the ... suscep-tibility to inhibitors. Furthermore, mutants selected withone inhibitor are often cross-resistant to other inhibitors(reviewed in [47]). The number of mutations increases with the time of therapy....

Tài liệu Báo cáo khoa học: "Effect of Cross-Language IR in Bilingual Lexicon Acquisition from Comparable Corpora" pot

... this distribution for the correctbilingual term pairs, which are contained in the topmost 200 TP(tE) and are found in the existingbilingual lexicon (we have similar results for other sites ... evaluated sim-ply consulting a bilingual lexicon instead of employing anMT software. As reported in Collier and others (1998), the precision of simple word by word query translation with abilingual ... of cross-language retrieval of relevant texts is quiteeffective in improving the performance of preciseestimation of bilingual term correspondences.2 Acquisition of Bilingual TermCorrespondences from...

Tài liệu Báo cáo khoa học: Parvalbumin deﬁciency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of ﬁber speciﬁc proteins doc

... After extensive washing with NaCl ⁄ Tris,PV-positive ﬁbers were visualized by incubation of sectionswith 3,3¢ -diaminobenzidine (DAB) and analysis of immuno-stained sections.Cardiolipin measurementsTotal ... chronic exercise [8]. The mechanism of control of mitochondrial biogenesis in muscles in response to muscle activity, as well as the mechanism controlling differential biogenesis of mito-chondria ... calcineurin (CaN) [13] and calmodulin kinase[14] to play an important role. In addition, a recentstudy indicates an involvement of Ca2+signaling in the control of both mitochondrial biogenesis and...

Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf

... preparation [28].DiscussionProtein identiﬁcation is greatly facilitated by the growing number of genome sequences. To get furtherinsight into the function of a protein, additional infor-mation ... N-terminusinstead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intactprotein at its a-amino group and represents the aminoterminal amino acid of ... four-fold protonated peptide ion. The charge state of thispeptide is unexpected: a peptide containing two basicamino acids (arginine and histidine) and a blockedamino terminus should not acquire more...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... further assayedby ﬂow cytometry with annexin V–FITC ⁄ pyridineiodination (PI) double staining, which identiﬁed apop-totic cells as annexin V positive, but PI negative. Thismethod obtained ... Hsp70-medi-ated protection against H2O2-induced apoptosis is notclear. In the present investigation, we further studied the effects of nucleolin ⁄ C23 ablation on Hsp70-medi-ated protection ... effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells. FEBS J 276, 2615–2624.25 Stankiewicz AR, Livingstone AM, Mohseni N &Mosser...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... domain can inhibit a linkedactivation domain, and this inhibition is not limited to the Meis2 activationdomain. Database searching reveals that the Meis3.2 splice variant, whichis found in ... transcriptional activationdomain (AD), the activity of which is inhibited by the Hth domain. This autoinhibition can be relieved, in part, by interaction with Pbx1, and maps to a region of the Hth domain that ... other DNA contacts outside helix 3 [4–7]. In addition to binding DNA, the HD is a protein interac-tion module that mediates interactions with otherDNA-binding proteins and non-DNA-binding tran-Keywordshomeodomain;...

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