Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc

... o AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus 119 119 119 119 119 120 119 121 118 119 159 159 159 159 157...

Ngày tải lên: 19/02/2014, 16:20

... Ozawa Y, Nakamura T, Kamata N, Yasujima D, Urushiyama A, Yamakura F, Ohmori D & Imai T (2005) Thermococcus profundus 2-ketoisovalerate ferredoxin oxidoreductase, a key enzyme in the archaeal energy-producing ... results). The enzyme activity of the recombinant LDH was assayed at 70 ° C by monitoring the lactate-dependent NADH oxidation as the decrease in A 340 . The...

Ngày tải lên: 16/02/2014, 09:20

... position of the adenine ring in the ATP-binding site, as shown in Fig. 6. The oxy- gen atoms of the pantoate are spatially coincident with the nitrogen atoms of the adenine ring. The O1, O2 and O4 atoms ... between the O2 atom of pantoate and the Nf atom of the side chain of Lys39. However, there are a large number of water-mediated networked interactio...

Ngày tải lên: 16/02/2014, 09:20

... corresponding to the targeting siRNAs is: HIF- 1a: 5¢-TCGACAAGCTTAAGAAAGA-3¢; VHL: 5¢-CCAAGACACCTCGAGAAT-3¢ (Ribobio, CN). The scrambled siRNA sequence used as negative control is 5¢-GAGUAGAAGAUUCAAGCUU-3¢ ... Statistical evaluation revealed that the amount of HIF- 1a was significantly increased in parallel with increasing glioma grade (Fig. 7B). The percentage of HIF- 1a- posit...

Ngày tải lên: 18/02/2014, 13:20

... the cells entered G 1 and then increasing again as the cells approached and entered S phase. The same pattern of variation of iPLA 2 activity was detected whether the assay was conducted with mixed micelles, ... cells, and compared it to that of another major intracellular PLA 2 , the AA-selective cPLA 2 a. Utilizing various strategies, we demonstrate here that iPLA 2 - VIA,...

Ngày tải lên: 18/02/2014, 17:20

... evolutionary aspects of invertebrate tachykinin and tachykinin-related peptide Atsuhiro Kanda, Kyoko Takuwa-Kuroda, Masato Aoyama and Honoo Satake Suntory Institute for Bioorganic Research, Osaka, Japan Tachykinins ... into tachykinin-related peptides, their receptors, and invertebrate tachykinins: a review. Zool Sci 5, 533–549. 7 Satake H, Ogasawara M, Kawada T, Masuda K, Aoy- ama M, Min...

Ngày tải lên: 19/02/2014, 00:20

... active sites of these enzymes contain a unique catalytic triad, Ser-Glu-Asp, in place of the canonical Ser-His-Asp triad of the classical serine peptidases. In the latter case, the Ser and His residues act ... percentage intensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity o...

Ngày tải lên: 19/02/2014, 07:20

... observed after germline inactivation. Inactivation of MR in the forebrain leads to impaired hippocam- pal-dependent learning, but the limbic MR is dispensable for the maintenance of basal hypothalamic–pituitary–adrenal ... transcription of genes involved in hepatic, renal, and intestinal detoxification and genes of the innate and adaptive immune system. REV-ERBalpha, a...

Ngày tải lên: 19/02/2014, 07:20

... and pET-PDE1 as template. PDE1(Arg189–Thr620) was amplified using the primer pairs 5¢-GGGAATTCCATATGAGAGACAATA TTTCCCGTTTATCAAATC-3¢ and 5¢-CCGCTCGAGT CATTACTAGGTTCCCTGTCCAGTGTTACC-3¢,and PDE1(Lys321–Thr620) ... maintaining the stability of the enzyme, or if it modulates the activity of the catalytic domain. Similar experiments with another trypanosomal PDE, TbPDE 2A, have demons...

Ngày tải lên: 19/02/2014, 12:20

... minichromosomes. Structural alterations in nucleosomal organization caused by TSA treatment Changes in the acetylation status of histones by TSA treatment may cause changes in the organization of chromatin. Such altered ... inhibitors such as TSA may change the bulk acetylation pattern of histones, and in this way alter the structure of chromatin incorpor- ating them....

Ngày tải lên: 19/02/2014, 12:20