Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... con- centrations of tumor necrosis factor-a and interleukin-6, and increased hepatic mRNA levels of tumor necrosis factor-a and interleukin-6 were decreased by depletio...

Ngày tải lên: 14/02/2014, 18:20

... have indi- cated that the CagA PY -induced cell elongation pheno- type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well- known actin -binding proteins ... phosphatases involved in this scenario, however, remain unknown and do not require Shp2. Instead, CagA PY can inhibit Src activity both by direct interaction of both proteins or by...

Ngày tải lên: 14/02/2014, 19:20

... between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse keratinocyte cell line upregulation of CERK expression by activa- tion of PPARb results in a decrease ... survival resulting from activation of PPARb. Activation of PPARb by LD induces CERK mRNA expression and increases CerK activity in SP1 cells To investigate the ro...

Ngày tải lên: 18/02/2014, 18:20

... pump and in vitro vacuolar acidification in hepatoma HepG2 cells. The vacuolar H + - ATPase inhibitor bafilomycin and the cathepsin D inhibitor pepstatin A partially inhibited, both in vivo and in ... prepared from CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractions isolated 5 min postinjection of CT-B, and this increase wa...

Ngày tải lên: 19/02/2014, 02:20

... AP1 DNA binding activity increased within 1 h following EGF treatment and peaked at 3 h following the treatment (Fig. 5A). We also examined the effect of PD98059 on this binding activity and, as expected, ... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun ex...

Ngày tải lên: 19/02/2014, 06:20

... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins. However, two other positively charged amino ... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a sing...

Ngày tải lên: 19/02/2014, 13:20

... 3525– 3601. Ó FEBS 2004 HIV-1 Protease Inhibitors (Eur. J. Biochem. 271) 4461 Role of hydroxyl group and R / S configuration of isostere in binding properties of HIV-1 protease inhibitors Hana Petrokova ´ 1 , ... on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f...

Ngày tải lên: 19/02/2014, 16:20

... chain into N-terminal and C-terminal centers during refolding [21]. Since then, many scientists have been interested in demonstrating this concept by using N-terminal and C-terminal fragments of ... located in the C-terminal loop of SNase plays an important role in protein stability and conforma- tional integrity. During protein unfolding ⁄ refolding, the addition of 2.5 p...

Ngày tải lên: 20/02/2014, 01:20

... periods of times (0–60 min) at 37 °C. The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of ... measuring the binding of [ 125 I]Streptavidin [30]. No Ig binding was observed in cells transfected with the vector alone (Fig. 4B). As shown in Fig. 4B, quantification of...

Ngày tải lên: 21/02/2014, 00:20

... occur during the initial 6 h window of infection. We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 h postinfection, and examined the cells ... fully understand HSV-1 infection of all target cells, includ- ing RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing no...

Ngày tải lên: 18/02/2014, 14:20