Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... 2004 Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics Ibrul ... the expression of the CTGF/CCN2 gene in smooth muscle cells. Keywords: actin cytoskeleton; CTGF/CCN2; p38 MAP kinase; Rho GTPase; smooth muscle cells. Connective tissue growth factor (CTGF) also ... in CA-Cdc42-transfected cells indicating a preponderant role of p38 in Cdc42 signaling as well.Role of RhoA GTPase and p38 in transcriptional and post-transcriptional regulation of theCTGF/CCN2 gene In order...

Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc

... release of cytochrome c from mitochon-dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3,the phosphoserine ⁄ threonine binding proteins in ... pro-caspases are often present in the cytosolfraction (caspase-2, -3, -6, -7, -8 and -9) of living cells to separate silent precursor caspases in the cytosolfrom pro-apoptotic cofactors in the mitochondria ... activation [65].MAPK pathways consist of three major kinases:the activation of p38 MAPK, the extracellular signal-regulated kinases (ERK) and Jun NH2 terminal kinases(JNK) [66]. JNK and p38 MAPK activation...

Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc

... 283,29961–29970.107 Trinklein ND, Chen WC, Kingston RE & Myers RM(2004) Transcriptional regulation and binding of heatshock factor 1 and heat shock factor 2 to 32 humanheat shock genes during thermal ... shock factors are involved in the regulation of life span and progression of cancer and they are also important fordevelopmental processes such as gametogenesis, neurogenesis and mainte-nance of ... isfacilitated by transcription factors, the single largest group of proteins in humans. For cell- and stimulus-speciﬁc gene regulation, strict control of the transcription factors themselves is...

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... and neutral pH [22].The latter act as competitive inhibitors, binding revers-ibly into the active site of the enzyme. Cystatins,including human cystatin C, are general inhibitors of cysteine ... whichthe N-terminus of the inhibitor ﬁrst binds to theenzyme, displacing the occluding loop, followed by thebinding of another two loops of the inhibitor [24].Besides protein inhibitors, the irreversible ... Increasing the concentration of 2A2 mAbat a ﬁxed molar ratio of cathepsin B and cystatin Cresulted in weaker bands of the cathepsin B ⁄ cystatin Ccomplex and stronger bands corresponding to...

Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... substrate and inhibitor for binding to thesame site [12]. We have previously determined thestructures of wild-type dCTP deaminase in complexwith dUTP and the inactive E138A mutant protein in complex ... designated A and B,originating from the A and B chains in the structure.dTTP binds at the site of the protein shown previously to bind the nucleotides dUTP and dCTP in wild-type and the E138A ... hyperbolic binding of ligands to the enzymeN ¼ Nmax½L=ðKdþ½LÞ ð3Þwhere N is the degree of binding with the dissociation con-stant Kd of ligand L to the enzyme with a maximal number of binding...

Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc

... conditio ns: actin alone (Actin) ; actin + T800 (Actin +T800); actin + Tm–Tn + CaCl2 (Actin + T m-Tn); actin + Tm–Tn + T800 + CaCl2 (Actin + Tm–Tn + T800). STOPS corres-pond to the time ﬁlaments ... ctin, M protein,C protein, MURF-1, calpain 3, myomesin, a-actinin,nebulin, telethonin and obscu rin.The elastic domains ar e made o f t andemly arrangedimmunoglobulin ( Ig)-like domains and ... more than 45% of T800remains bound to actin, support the in vivo ext rapolation o ftitin binding to actin in the sarcomeric I-band region and itsfunctional consequences in striated muscle. However,...

Tài liệu Báo cáo khoa học: Modulation of sterol homeostasis by the Cdc42p effectors Cla4p and Ste20p in the yeast Saccharomyces cerevisiae pptx

... plasmidcombinations were spotted either onto medium lacking histidine and leucine to select for the plasmids, or onto medium lacking histi-dine, leucine and uracil to monitor protein interactions. ... pro-tein–protein interaction therefore results in nongrowth on mediumlacking uracil. (B) In contrast to Ste20p, Cla4p does not bind to Erg4p, Cbr1p or Ncp1p. A total of 105 cells of the indicated ... Thesplit-ubiquitin system. The N-terminal and C-terminal halves of ubiquitin (N-Ubi and C-Ubi) alone do not assemble. If a protein ‘X’,fused to N-Ubi, binds to the PAKs Ste20p or Cla4p, linked to C-Ubi,both...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... tumorigenesis and genetic diseases[47].Most analyses of the regulation of AP-2 and theinteractions of the transcription factor with bindingpartners, as well as of the regulation of target gene expression, ... chondrogenesis. Overview of the differen-tiation stages during chondrogenesis and the involvement of transcription factors(henatoxylin and eosin-stained section of anembryonic cartilaginous ... development, including transcription factors. Essential transcription factors in chondrocytedifferentiation are Sox9 and Runx2. Sox9 plays a keyrole in chondrogenesis, as an inactivating mutation in the...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... indicate intron 6 of 75 bp in fragmentsB and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number atthe right of each RT-PCR ... entirecoding region. Thus, use of alternative promoters in DNASE1 results in no generation of protein isoforms.The 5¢-UTR of eukaryotic mRNA in uences the initi-ation step of protein synthesis and ... faster-migra-ting faint bands. Alternative splicing occurs in manygenes, and so DNA fragments were puriﬁed from themajor band and cloned into a sequencing vector. DNAsequences were determined for...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... channel. This removesthe troponin inhibition of the actin myosin interaction, and induces muscle contraction. When the muscle relaxes, Ca2+needs to be removed from the cytoplasmby the Ca2+-pump ... single line scan data obtained with buffer conditions. (A) Representative single line scan graphs obtained at increasing ATPconcentration in the range 0–100 lM and in the presence of 10 nM and ... imaging of SERCA dynamics in the presence of nucleotide and Ca2+. (A) Time-lapse sequence FSAFM images of SERCA in the SR membrane on a mica surface in a buffer solution were obtained in the...

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