Tài liệu Báo cáo khoa học: Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results in a reduced bacterial glycogen accumulation docx

... of yeast glycogenin (Glg2p) in E. coli (Eur. J. Biochem. 271) 3989 Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results ... the eukaryotic glycogenin and bacterial glycogen accumulation The data s hown in Figs 3–5 clearly indicate t hat the transformed E. col...

Ngày tải lên: 19/02/2014, 16:20

... 185–190. 6 McIntosh EM & Haynes RH (1984) Isolation of a Saccharomyces cerevisiae mutant strain deficient in deoxycytidylate deaminase activity and partial charac- terization of the enzyme. J Bacteriol ... (yellow; chain B), dUTP (cyan; Protein Data Bank entry 1XS4, chain A) , or dCTP (magenta; Protein Data Bank entry 1XS6, chain A) . The nucleotides are shown in ball and stick rep...

Ngày tải lên: 18/02/2014, 16:20

... c (12.4 kDa), ovalbumin (44 kDa), bovine serum albumin (67 kDa), aldolase (158 kDa) and catalase (232 kDa) were used as standards to calibrate the gradient and are indicated as squares in the gradient. (B) ... T-COFFEE. Amino acids characteristic of Hfq are indicated in black. Amino acids in light grey are conserved in most Hfqs and located in the b-strands. Acidic amino acids at...

Ngày tải lên: 19/02/2014, 12:20

... double stranded oligos Oct-1, 5¢-TGT CGAATGCAAATCACTAGAA-3¢; Sp1, 5¢-ATTCGATC GGGGCGGGGCGAGC-3¢; Ets/Pea3, 5¢-GATCTCGAG CAGGAAGTTCGA-3¢; Ets (PU.1), 5¢-GGGCTGCTTG AGGAAGTATAAGAAT-3¢;Stat3,5¢-GATCCTTCTG GGAATTCCTAGATC-3¢; ... 5¢-GGGCTGCTTG AGGAAGTATAAGAAT-3¢;Stat3,5¢-GATCCTTCTG GGAATTCCTAGATC-3¢; and C/EBP, 5¢-TGCAGATT GGGCAATCTGCA-3¢ are from Santa Cruz Biotechno- logy. The binding reactions...

Ngày tải lên: 20/02/2014, 11:20

... that originate from (a) natural abun- dance in non-C-atoms, (b) natural abundance of 13 Cinthe derivatization reagent, and (c) 13 C in the carbon skeleton of the amino-acid fragment that were incorporated ... was expected, as the fractions of pyruvate originating from malate and PEP originating from OAA that are indicative of in vivo malic enzyme and PEP carboxykinase activity, respecti...

Ngày tải lên: 20/02/2014, 23:20

... expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinflammatory cytokines, leading to a loss of catalytic activity. This downregulation was at ... significantly at 24 h after CLP and this increase was attenuated by GdCl 3 . The malondialdehyde (MDA) level in CLP rats was 1.8-fold that of sham-operated rats. The increase i...

Ngày tải lên: 14/02/2014, 18:20

... cycle of regulated single-stranded RNA binding affinity transitions, so that protein and RNA dissociate [44]. RNA chaperones and annealers RNA annealer proteins are able to accelerate anneal- ing of ... annealing activity, it can catalyze the strand displacement event in two ways: by destabilizing edges and bulges, and by favoring the annealing reaction of the invading strand. A clear ad...

Ngày tải lên: 14/02/2014, 19:20

... Horse- radish peroxidase-conjugated antibody against caspase-3 (#610325) was from BD Pharmigen, and antibody against b-actin (A5 441) was from Sigma. Statistical analysis All data are expressed as ... examined the main steps involved, following the uptake of satu- rated and unsaturated FFAs into the cells. After their internalization, FFAs are converted to fatty acyl-CoA, a reaction catalyz...

Ngày tải lên: 14/02/2014, 22:20

... Miyazawa K, Tsubouchi H, Naka D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy- ama O, Takahashi K et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth ... Technology, Nagatsuta, Midori-ku, Yokohama, Japan 2 Advanced Medical Research Laboratory, Mitsubishi Tanabe Pharma Corporation, Kamoshida-cho, Aoba-ku, Yokohama, Japan Introduction Type...

Ngày tải lên: 15/02/2014, 01:20

... 7p12-13 and codes for a 170kDa receptor tyrosine kinase. All ERBB proteins have four functional domains: an extracellular ligand-binding domain; a transmembrane domain; an intracellular tyrosine kinase ... kinase domain; and a C-terminal regulatory domain [2]. The extracellular domain is subdivided further into four domains. The tyrosine kinase domain consists of an N-lobe and...

Ngày tải lên: 16/02/2014, 09:20