... kinetic analysis of these inhibitingor activating effects was either incomplete [22,36–39]or debatable [33,42]. In particular, reported resultswere interpreted in terms of an AAA site distinct ... observationsobtained (Table 1).The binding of tyramine to D70G, in the presence of o-NPA, is weaker (9.1-fold) than binding to thewild-type enzyme, and induces an activating effect ono-NPA ... comparison of the effects of tyramine and serotonin on D70G versus the wild-typeenzyme indicated that: (a) the peripheral anionic site is involved in the non-linear activation and inhibition of the...