Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc

... Critical roles of Asp40 at the haem proximal side of haem- regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control Miki Watanabe, Hirofumi ... affect the exogenous ligand binding access channel a t t he haem distal side and/ or structure in t erms of cyanide binding in the Fe(III) comp...

Ngày tải lên: 19/02/2014, 16:20

... metabolism in the cold. Mathemati- cal modelling and simulation of cold-induced metabolic reprogramming indicated major differences in the rates of interconversion between the pools of hexoses and sucrose, ... 6- phosphate and 40 mm glucose 6-phosphate; 30% KOH was added to the control of each assay. Reactions were incubated for 30 min at 25 and 4 °C, and the...

Ngày tải lên: 14/02/2014, 22:20

... prominent path- ways are the extrinsic and intrinsic pathways. In the intrinsic pathway (also known as the ‘mitochondrial pathway’), apoptosis results from an intracellular cas- cade of events in ... from H 2 O 2 -mediated injury [12]. Therefore, in this study, we investigated whether HSS could function as an MPT inhibitor, thereby alle- viating hepatic injury and promot...

Ngày tải lên: 16/02/2014, 09:20

... of PPARc tar- get genes is increased in WAT of TG mice, suggesting that PPARc might be activated more in WAT of TG mice than in WAT of WT mice. Serum levels of triglyceride, glucose, leptin and insulin, ... we show, for the first time, that PGD 2 is involved in the activation of adipogenesis and regula- tion of insulin sensitivity in vivo. Further characteriza-...

Ngày tải lên: 16/02/2014, 09:20

... DTNB) Metals in MTs are relatively deeply buried in the pro- tein. The modification of the surface-accessible sulfur of cysteine ligands is thought to be the key that unlocks the metals from the protein ... ligands. By contrast, the slow-reacting cisplatin had all ligands replaced with thiolates. Cisplatin binds preferentially to the b-domain (but binding with transpl...

Ngày tải lên: 16/02/2014, 15:20

... 3665 (ruling out the possibility of an autocrine, paracrine or endocrine mechanism of regulation in the ob ⁄ ob model), and given that the majority of leptin receptors exist in the arcuate nucleus of ... was dramati- cally reduced in sympathectomized animals, indicating the sympathetic origin of these nerve endings [22]. Given the distribution of the NPY-posi...

Ngày tải lên: 18/02/2014, 04:20

... an indication that the former is closer to a SIM than the latter. Using these plots and having measured the concentration of EIIAÆP, the investigator can read the values of the remaining eight ... limitation is retreating, whereas the importance of accurate modeling and analy- sis approaches aiming at understanding the complex interactions in living cells is incr...

Ngày tải lên: 18/02/2014, 06:20

... denominator of Eqn (5). Based on the SD of the numerator and the denominator the SEM of q h was computed, assuming statistical independence of the two. The time-dependent metabolic regulation ... to the new steady state [5,8,9]. In order to obtain insight into adaptation strategies of organisms, it would be more informative to follow the patterns of regulation...

Ngày tải lên: 18/02/2014, 06:20

... with CUL3 and other BTB proteins [2,16], it remains unclear what kind of interactions are mediated by the MATH domain in plants. In this article, we show that the MATH domain of BPM proteins is used ... that Arabidopsis BPM proteins use their BTB ⁄ POZ domain to interact with the cullins CUL3A and CUL3B [3,4,16], we inves- tigated what kind of protein–protein interactions...

Ngày tải lên: 18/02/2014, 06:20

... chondrocytes. In the absence of ALLN, smears of ubiquitinated proteins were not observed in control and TRAIL-treated canine chondrocytes. In the presence of ALLN, marked accumulation of polyubiquitinated ... Taken together, our data indicated that proteasome inhibition enhanced TRAIL-induced cell death via the caspase pathway, the key regulator of the TRAIL-indu...

Ngày tải lên: 18/02/2014, 14:20