Tài liệu Báo cáo khoa học: Critical roles of Asp40 at the haem proximal side of haem-regulated phosphodiesterase from Escherichia coli in redox potential, auto-oxidation and catalytic control doc
... Critical roles of Asp40 at the haem proximal side of haem- regulated
phosphodiesterase from
Escherichia coli
in redox potential,
auto-oxidation and catalytic control
Miki Watanabe, Hirofumi ... affect the exogenous ligand binding access
channel a t t he haem distal side and/ or structure in t erms of
cyanide binding in the Fe(III) comp...
... metabolism in the cold. Mathemati-
cal modelling and simulation of cold-induced metabolic reprogramming
indicated major differences in the rates of interconversion between the
pools of hexoses and sucrose, ... 6-
phosphate and 40 mm glucose 6-phosphate; 30% KOH
was added to the control of each assay. Reactions were
incubated for 30 min at 25 and 4 °C, and the...
... prominent path-
ways are the extrinsic and intrinsic pathways. In the
intrinsic pathway (also known as the ‘mitochondrial
pathway’), apoptosis results from an intracellular cas-
cade of events in ... from H
2
O
2
-mediated injury
[12]. Therefore, in this study, we investigated whether
HSS could function as an MPT inhibitor, thereby alle-
viating hepatic injury and promot...
... of PPARc tar-
get genes is increased in WAT of TG mice, suggesting
that PPARc might be activated more in WAT of TG
mice than in WAT of WT mice.
Serum levels of triglyceride, glucose, leptin and
insulin, ... we show, for the first time, that PGD
2
is
involved in the activation of adipogenesis and regula-
tion of insulin sensitivity in vivo. Further characteriza-...
... DTNB)
Metals in MTs are relatively deeply buried in the pro-
tein. The modification of the surface-accessible sulfur
of cysteine ligands is thought to be the key that
unlocks the metals from the protein ... ligands. By contrast, the
slow-reacting cisplatin had all ligands replaced
with thiolates. Cisplatin binds preferentially to the
b-domain (but binding with transpl...
... 3665
(ruling out the possibility of an autocrine, paracrine or
endocrine mechanism of regulation in the ob ⁄ ob
model), and given that the majority of leptin receptors
exist in the arcuate nucleus of ... was dramati-
cally reduced in sympathectomized animals, indicating
the sympathetic origin of these nerve endings [22].
Given the distribution of the NPY-posi...
... an
indication that the former is closer to a SIM than
the latter. Using these plots and having measured the
concentration of EIIAÆP, the investigator can read the
values of the remaining eight ... limitation is retreating,
whereas the importance of accurate modeling and analy-
sis approaches aiming at understanding the complex
interactions in living cells is incr...
... denominator of Eqn (5). Based
on the SD of the numerator and the denominator the SEM
of q
h
was computed, assuming statistical independence of
the two. The time-dependent metabolic regulation ... to the new steady state [5,8,9]. In order to
obtain insight into adaptation strategies of organisms,
it would be more informative to follow the patterns of
regulation...
... with
CUL3 and other BTB proteins [2,16], it remains unclear
what kind of interactions are mediated by the MATH
domain in plants.
In this article, we show that the MATH domain of
BPM proteins is used ... that Arabidopsis
BPM proteins use their BTB ⁄ POZ domain to interact
with the cullins CUL3A and CUL3B [3,4,16], we inves-
tigated what kind of protein–protein interactions...
... chondrocytes. In the
absence of ALLN, smears of ubiquitinated proteins
were not observed in control and TRAIL-treated
canine chondrocytes. In the presence of ALLN,
marked accumulation of polyubiquitinated ... Taken
together, our data indicated that proteasome inhibition
enhanced TRAIL-induced cell death via the caspase
pathway, the key regulator of the TRAIL-indu...