Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf

... shown). Mapping of the NS5B- binding site on the CSFV genome The NS5B protein is the replicase for the C SFV genome. Therefore, all sites of the full-length CSFV genome could be bound for the NS5B protein. ... Specific interaction between the classical swine fever virus NS5B protein and the viral genome Ming Xiao 1,2 , Jufang Gao 2 , Wei...

Ngày tải lên: 19/02/2014, 16:20

... nonspecific electrostatic interactions between the negatively charged head groups of the phospholipids, which include the carboxyl group of PS and the phosphoryl groups of phosphoinositides, and Fig. ... 2006 The Authors Journal compilation ª 2006 FEBS the positively charged residues of I C initially attract the inhibitor to the membrane surface, and that the membrane...

Ngày tải lên: 19/02/2014, 05:20

... incubated with the reactivase in the presence of ADP and Mg 2+ and followed by nondenaturing PAGE, the bands of the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes ... a R Æa R b R and b R in the presence of ADP and Mg 2+ was observed even without the enzyme. The crystal structure of the reactivase al...

Ngày tải lên: 15/02/2014, 01:20

... respec- tively. The degrees of homology in the C-terminal reg- ulatory domain are relatively low, being 33, 24 and 28%, respectively. Structure of the ERBB proteins and diversity of their ligands The EGFR ... been reported in the extracellular domain of the EGFR gene [6]. In the EGFR vI mutation, the extracellular domain has been totally deleted and resembles the v-erb...

Ngày tải lên: 16/02/2014, 09:20

... protons by the protein or the ligand. If this is the case, the bind- ing enthalpy is dependent on the ionization enthalpy of the buffer in which the reaction takes place. ITC exper- iments were therefore ... indicated a slow exchange on the NMR timescale between the free and the bound forms for NarJT. These results and the higher excess partial molar heat capacit...

Ngày tải lên: 16/02/2014, 14:20

... direct interaction between ETA and cyto- solic EF-2 on the one hand, and the mitochondrial membrane on the other hand. However, the potential role (if any) of ADP-ribosylation of EF-2 in the mitochondrial ... protease cathepsin D, as indicated by the following observa- tions: (a) the inhibitor profile of the endosomal ETA-degrading activity was very similar to that of c...

Ngày tải lên: 18/02/2014, 04:20

... but, rather, there are interactions that exist between dimer partners all along the ectodomain of the receptor. Collectively, these studies led to the hypothesis that production of high-affinity ligand ... difference between Glc-6-P and Man-6-P is the position of the 2-hydroxyl group. The equatorial 2-hydroxyl group of Glc-6-P is not in the correct posi- tion to make the...

Ngày tải lên: 18/02/2014, 08:20

... transcript abundance, the intensity of the amplified PCR bands was determined using imagej. Relative mRNA expression levels from each of the samples were presented as the ratio of the band intensities of the VgR ... Membrane proteins were extracted from virgin and mated queens and males of unknown age (Figs 1 and 2A). To confirm receptor tissue -specific expression, membrane p...

Ngày tải lên: 18/02/2014, 08:20

... of the main chain and subse- quent disorganization of the central b-sheet. Moreover, catalysis relies on optimal angles and distances between the nucleophile and the base in order to allow the ... 0.01 lm, and that of the D70G mutant was 0.196 ± 0.02 lm. Effects of ligands on steady-state kinetics The effects of the ligands (L: tyramine, serotonin and ben- zalkoniu...

Ngày tải lên: 18/02/2014, 17:20

... shape between stefins A and B and the P79S mutant. The two isoforms of stefin B have exactly the same far UV CD. Regardless of the sequence differences (as highlighted in Fig. 1), the secondary and ... no difference in the ratio between the oligomers is seen, whereas after incubation at 36 °C there is a marked shift from the monomer towards the dimer (and some tetra...

Ngày tải lên: 19/02/2014, 06:20