Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... 3325 Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunae as revealed by reversible cofactor dissociation studies Richard ... on catalytic activity and stability. We chose starch phosphorylase from Corynebacterium callunae (CcStP), which has been charact...

Ngày tải lên: 19/02/2014, 16:20

... MAP2 cDNA and its derivatives generated by PCR were cloned into pGEX-4T-2. The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2. The N- and C-terminal ... Sigma-Aldrich) and HA (catalog number: 1867423; Roche Diagnostics, Basel, Switzerland) were used at dilutions of 1 : 1000 for immunoblotting, 1 : 200 for immunocytochemi...

Ngày tải lên: 14/02/2014, 19:20

... residues and solvent molecules engaged in ligand recognition and stabilization are drawn as balls -and- sticks and spheres, respectively. The major interactions established between the DHAP inhibitor and ... cross-rotation and translation functions in the 10–4 A ˚ resolution range. The solution of the rotation function was used to perform the translation function calculations...

Ngày tải lên: 18/02/2014, 06:20

... is inhibited by ATP depletion; by vanadate, which has been shown to inhibit Pgp, MRP1 and MRP2 [32–34]; by MK571, which has been shown to inhibit MRP1, MRP2, MRP4 and MRP7 [26,27,35,36]; and by indo- methacin, ... was inhibited by 4-chloro-7-nitro- 2,1,3-benzoxadiazole (NBD-Cl) and MK571 and, to a lesser extent, by ATP depletion, indomethacin, probenecid and vanadate. A...

Ngày tải lên: 18/02/2014, 13:20

... required: for BM27.6, erythromycin (1 lgÆmL )1 ); for MC25 and 90-226emm1::km, kanamycin (150 lgÆmL )1 ); for BMJ71, tetracycline (5 lgÆmL )1 ); for JRS4 and JRS145, streptomycin (100 lgÆmL )1 )andfor JRS145/pLZM6 ... purified from growth media of AP1 bacteria as described [32]. Polyclonal human IgG, albumin and fibrinogen were purchased from Sigma. Chondroitinase ABC (EC 4.2....

Ngày tải lên: 20/02/2014, 11:20

... bonds formed by hydroxyl groups (O–HÆÆÆO,  21 kJÆmol )1 ) are twice as strong as those formed by the amide group [21]. Furthermore, hydrogen bonds formed by the sin- gle hydroxyl donor in Ser and ... should form a rather rigid and hydrophilic peptide. The abundance of Ser and Thr residues in linkers from animals also provides numerous potential targets for O-linked glycosylat...

Ngày tải lên: 14/02/2014, 18:20

... overexpression, parkin and PTEN-induced putative kinase 1 loss-of -function and UCHL1 mutation, lead to an impairment of neuronal dopamine homeostasis by interfering with the vesicular storage and release mechanisms. ... with GO classi- fications was obtained from ppi spider starting from the proteins grouped as above (Tables S2 and S3). In both cases, bioinformatic analysis re...

Ngày tải lên: 15/02/2014, 01:20

... have been pre- pared for studies on the structure and function of PrP C , and which are now available upon request for use elsewhere. The data are collected in Tables 1 and 2, and at the end of ... for 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion...

Ngày tải lên: 18/02/2014, 08:20

... known as functional hyperemia. Cerebral blood flow rate and vessel diameter are not fixed in spe- cific regions, but depend on the local demand for oxygen and nutrients by synaptic transmission and neuronal ... and ions is accom- panied by the movement of water, which is buffered by astrocytes. The increased Na + concentration caused by glutamate transport, and increased intr...

Ngày tải lên: 18/02/2014, 11:20

... in both embryogenesis and adulthood, and functions by binding with two tyrosine kinase recep- tors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR ⁄ Flk-1), Fig. 1. The VEGF–VEGFR system and the inhibitors of ... by Grant-in-Aid Special Pro- ject Research on Cancer-Bioscience 17014020 from the Ministry of Education, Culture, Sports, Science and Technology of Japan, a grant from the progra...

Ngày tải lên: 18/02/2014, 11:20