Tài liệu Báo cáo khoa học: Calcium-independent cytoskeleton disassembly induced by BAPTA pdf

... active fractions was then con- firmed by SDS–PAGE. Standard enzyme assay Protein concentration was determined by the Bradford method using BSA as standard, or by UV-vis spectroscopy (e 600 nm =5· ... hybrid (lac131, lac232 and lac535) sequences are represented by rectangles of variable lengths. Recombinant junctions are indicated by vertical bars. (B) Representation of cupredoxin dom...

Ngày tải lên: 18/02/2014, 06:20

... plasma membrane allows polar- ity to be maintained by endocytic cycling. Curr Biol 13, 1636–1640. M. Lin et al. Sterol homeostasis modulation by Ste20p and Cla4p FEBS Journal 276 (2009) 7253–7264 ... homeostasis modulation by Ste20p and Cla4p M. Lin et al. 7264 FEBS Journal 276 (2009) 7253–7264 ª 2009 The Authors Journal compilation ª 2009 FEBS Modulation of sterol homeostasis by the...

Ngày tải lên: 18/02/2014, 13:20

... synthesis and febrile response induced by SEC1 is dependent on NADPH oxidase and not on PI3K ⁄ Akt or 5-LOX ⁄ FLAP because these phenomena are attenuated by apocynin and not by ETYA, MK 886, Wort or ... 3637 nuclear translocation (Fig. 2C, lanes 5–7). The DNA- binding activity of NF-jB induced by SEC1 was com- pletely blocked by 1000 lm of PDTC (Fig. 2D, lane 7) or 10 lm of SN-5...

Ngày tải lên: 19/02/2014, 00:20

... environment at the nuclear periphery. This can be achieved by forming repressive complexes by the NE proteins as illustrated in Fig. 1. By serving as docking sites at the INM, LAP2b, LBR, and poss- ibly ... Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. J Cell Biol 145 , 1341–1354. 40 Ye Q, Hu YF, Zhong H, Nye AC, Belmont AS & Li R (2001...

Ngày tải lên: 19/02/2014, 02:20

... vol. 8, No. 2, February 1965] Evaluation of Machine Translations by Reading Comprehension Tests and Subjective Judgments by Sheila M. Pfafflin*, Bell Telephone Laboratories, Incorporated, ... machine translations of these passages were provided by IBM and were based on the Bidirectional Single-Pass translation system developed there by G. Tarnawsky and his associates. This syst...

Ngày tải lên: 19/02/2014, 19:20

... ligation by reverse-joined hairpin ribozymes Reverse-joined hairpin ribozymes, first introduced by Ohtsuka and colleagues [29,30], are derived from the conventional hairpin ribozyme by dissecting ... ⁄ HCl (pH 7.5) buffer was heated at 90 °C for 1 min followed by incuba- tion at 37 °C for 15 min. The cleavage reaction was started by addition of MgCl 2 and spermine. The final volume of...

Ngày tải lên: 20/02/2014, 01:20

... src="data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAxMAAAS6CAIAAACujKOvAAAACXBIWXMAABYlAAAWJQFJUiTwAAAgAElEQVR42uzdf4yUdWL48WfJeNdkV2tIZzNGiUvDWQrukCvXGne5Xs0NXPjR4lWOZgZr0hjSoisbu38YMWXdTVzTSzYe7FZNiGdijtm4wVQSWHowhpiwEGKs58zJ0TsqY1bD3E5KiTJNW6fl+8fT7pei4O6yu/Pr9foLUXaf+TzjzpvP83k+T9OVK1cCAGaiqanJIFSnWCx24cIF48D8iRgCgFnw105RS2NaZAgAAJQTAIByAgBQTgAAygkAQDkBACgnAACUEwCAcgIAUE4AAMoJAEA5AQAoJwAA...

Ngày tải lên: 20/02/2014, 04:20

... parse tree with all of its 11 subtree features covered by the convolu- tion tree kernel. In the tree kernel, a parse tree T is represented by a vector of integer counts of each subtree type ... ker- nel (Collins and Duffy, 2002), eq. (6) holds be- cause a common subtree by extending from ( 1 ,  2 ) can be formed by taking the hyper-edge ( 1 ,  2 ), together with a choice at...

Ngày tải lên: 20/02/2014, 04:20

Ngày tải lên: 20/02/2014, 04:20

... Our choice is on the one hand moti- vated by the extensive knowledge available on se- mantic change in English. On the other hand, our choice was driven by the availability of large cor- pora for ... senses are learned with LDA. Each sense cor- responds to one row and is described by the top five terms identified by LDA. The higher the gray area at a certain x-axis point, the more of th...

Ngày tải lên: 20/02/2014, 05:20