Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

Tài liệu Báo cáo khoa học: Evolutionary relationships of the prolyl oligopeptidase family enzymes docx

... alignment of studied POP family enzymes. Ó FEBS 2004 Evolutionary relationships of the POP family enzymes (Eur. J. Biochem. 271) 2715 Evolutionary relationships of the prolyl oligopeptidase family enzymes Jarkko ... ancestor of life. With respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved am...

Ngày tải lên: 19/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

... substitution of these two arginines. We map the site of binding of Ube4b to the N-domain of VCP and show that it competes for this site with Hrd1. Finally, we investigate the evolutionary divergence of the ... mediating binding both on the Ube4b–Ufd2p side [8] and on the VCP–Cdc48p side. We then investigated whether the binding of each of these VBM-containing pro...

Ngày tải lên: 18/02/2014, 13:20

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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... [26]. These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup- presses the release of the peroxide product. This may be one of the ... ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plan...

Ngày tải lên: 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... man- ganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... to the catalytic site of the R1E subunit. As reported in the Results, upon completion of substrate conversion, the radical is then rapidly passed back from...

Ngày tải lên: 15/02/2014, 01:20

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Tài liệu Báo cáo khoa học: Reductive nitrosylation of ferric human serum heme-albumin docx

Tài liệu Báo cáo khoa học: Reductive nitrosylation of ferric human serum heme-albumin docx

... 4 shows the dependence of the molar fraction of HSA-heme-Fe(II)-NO (i.e. Y) on the NO concentra- tion (i.e. [NO]). The value of Y increased linearly with the NO concentration, reaching the maximum (= ... [44]: k obs ¼ k on ½NOþk off ð2Þ The value of K (= k off ⁄ k on ; reaction (a) in Scheme 1) was determined from the dependence of the molar fraction of HSA-hem...

Ngày tải lên: 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino acids Lys, Thr, ... and B) sections of the structure are not seen on the map, probably owing to disorder of these regions. The over- all geometry of the model according to the p...

Ngày tải lên: 18/02/2014, 08:20

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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... that the activation of IRF-3 by M-pIC treatment is mediated by the TLR3 ⁄ TRIF signaling pathway. We obtained further evidence by examining the status of the phos- phorylation and dimerization of ... cells. First, we confirmed the effect of NS3-4A on the activation of the IFN-b gene promoter by the Cardif exogenously expressed in PH5CH8 cells. The results of the...

Ngày tải lên: 18/02/2014, 16:20

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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

... K d is the equilibrium dissociation constant of the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound to the ribozyme, and [ribozyme] u is the concentration of unbound ribozyme in the reaction. Measurement ... and fitting to the equation: k obs ¼ k 1 ½Eþk À1 : Measurement of the dissociation constant, K d P of the ribozyme–product complex The equilibrium di...

Ngày tải lên: 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... according to the residue in the mid- point of the respective loop, as shown in Fig. 2. To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase. The observed ... below). The southern boundary of the active site cleft of DESC1 is formed by the 145 autolysis loop. The backbone of this loop differs mark- edly from the oth...

Ngày tải lên: 19/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... determines the shape of the active site entry. (e) The structure of the active site is essentially identical with the active sites of the MshB and LpxC proteins. The conservation of catalytically important ... orients the C-terminal turn of the helix nearly perpendicularly to the rest of the helix. This turn of a5 is connected with a6-helix via a long loop...

Ngày tải lên: 19/02/2014, 00:20

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