Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... observed in the absence of changes in protein expression in rabbit he- patocytes [22]. ROS indirectly reduced the activity of selected isoforms of CYP b...

Ngày tải lên: 14/02/2014, 18:20

... control the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial for triggering ... distribution, making them unsuitable for the investigation of b-cate- nin signalling [29]. In support of this aspect, using Madin–Darby canine kidney cells that express E-ca...

Ngày tải lên: 14/02/2014, 19:20

... were obtained in ChIP DNA, indicating that the binding of putative CERK-PPRE to PPARb is specific. Furthermore, the interaction between PPARb and CERK was confirmed by an EMSA using biotin- labeled ... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that i...

Ngày tải lên: 18/02/2014, 18:20

... amounts of protein from 3Y1-Ad12 cell lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin ... bafilomycin and the cathepsin D inhibitor pepstatin A partially inhibited, both in vivo and in vitro, the cAMP response to cholera toxin. This cathepsin...

Ngày tải lên: 19/02/2014, 02:20

... AP1 DNA binding activity increased within 1 h following EGF treatment and peaked at 3 h following the treatment (Fig. 5A). We also examined the effect of PD98059 on this binding activity and, as expected, ... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and...

Ngày tải lên: 19/02/2014, 06:20

... Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP- N -acetylglucosamine enol pyruvyl transferase Alison ... with the K22V and K22E mutant proteins. This was interpreted as a lack of fosfomycin binding to these mutant proteins [12]. Binding of fosfomycin to...

Ngày tải lên: 19/02/2014, 13:20

... large in uence on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. ... 2.0 A ˚ . The overall layout of the inhibitor in the pr otease binding site is very similar for all the compared inhibitors. The side chains of all...

Ngày tải lên: 19/02/2014, 16:20

... measuring the binding of [ 125 I]Streptavidin. Specific binding at each point was nor- malized with reference to specific binding before the incubation at 37 °C (0-min point) to derive the fraction of ... biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of bTSH, a very weak internaliza...

Ngày tải lên: 21/02/2014, 00:20

... opening and cell swelling, an event that is probably reduced in extent by CsA. CsA specifically inhibits PTP opening by binding to cyclophilin D in the matrix and on the inner surface of the IM ... decreasing the activity of caspase-3 in the three cell lines. Effect of HSS knockdown on CCCP-induced apoptosis To further elucidate the functional role of HSS in...

Ngày tải lên: 16/02/2014, 09:20

... that the former is closer to a SIM than the latter. Using these plots and having measured the concentration of EIIAÆP, the investigator can read the values of the remaining eight concentrations off the ... ½HPr tot ¼ ~ z 2 þ ~ z 3 þ ~ z 8 þ ~ z 9 ; ½EIIA tot ¼ ~ z 3 þ ~ z 4 þ ~ z 10 þ ~ z 11 ; ½EIICB tot ¼ ~ z 4 þ ~ z 5 þ ~ z 12 þ ~ z 13 ð22Þ Using these, we can express...

Ngày tải lên: 18/02/2014, 06:20