Tài liệu Báo cáo khoa học: Computational approaches to understand a-conotoxin interactions at neuronal nicotinic receptors doc

... MINIREVIEW Computational approaches to understand a-conotoxin interactions at neuronal nicotinic receptors Se ´ bastien Dutertre and Richard J. Lewis Institute ... of computational tools in the field of nicotinic receptors has led to the publication of several models of ligand–receptor interactions. These models are all based on the crystal structure at ... and s...

Ngày tải lên: 19/02/2014, 12:20

... ligation; glycoMTS, glycosyl methanethiosulfonates; glycoSeS, selenenylsulfide-mediated glycosylation; MTS, methanethiosulfonates; NCL, native chemical ligation; PTM, post-translational modification; ... approach is to exploit the fine control that may be offered by chemistry [4]. A combination of chemical, enzymatic and biological augmentation strategies can provide a modification process that...

Ngày tải lên: 18/02/2014, 17:20

... like to be able to apply selective pressures that are not compatible with cellular survival (such as high temperature or denaturant). The problem is that there is another limitation to library approaches: ... the natural repertoire of amino acids is highly restrictive compared to the useful alterations that can be made to small molecules by physical organic chemists, and methods t...

Ngày tải lên: 19/02/2014, 12:20

... neuronal nicotinic acetylcholine receptor subtypes. Eur. J. Biochem. 271, 2305–2319. 3. Dutertre, S. & Lewis, R.J. (2004) Computational approaches to understand a-conotoxin interactions at ... GID. Table 1. Sequence, receptor specificity and structural information on neuronally active a-conotoxins. *Refers to the amidated C-terminus. IC 50 relates to receptors expre...

Ngày tải lên: 19/02/2014, 12:20

... immunoprecipitations as calibrators in combination with criteria-based normalization. Additionally, we developed a computational tool for automated normalization, validation and integration of data derived ... phosphorylated and total cytoplasmic STAT3 obtained by immunoblotting of cytoplasmic lysates, as well as immunoprecipitates (data not shown), demonstrated that our automated computa-...

Ngày tải lên: 19/02/2014, 07:20

... strategies when one is to study one particular se- mantic phenomenon in natural languages. The first one is to try to interpret the particular phenomenon into a system that attempts to capture ... mass terms. The strategy of isolating parts of a natural lan- guage and giving it a semantics that can be computational is of course the strategy underlying all computational use...

Ngày tải lên: 22/02/2014, 10:20

... caspases that induces DNA fragmentation [2]. The former pathway initiates on receipt of cell sur- face stimuli at the death receptors. These receptors, such as tumor necrosis factor receptor and ... the activation of which ultimately leads to cell death [40]. Two major pathways for caspase activation have been identified: the receptor pathway and the mito- chondrial pathway; both pathwa...

Ngày tải lên: 14/02/2014, 22:20

Ngày tải lên: 18/02/2014, 03:20

... demonstrated that hypoxic conditions prevented ROS generation and restored the loss of MTP seen after cotreatment with ALLN and TRAIL. These find- ings suggest that the mitochondrial respiratory ... MTP, whereas combined treatment with ALLN and TRAIL caused a significant dissipation of MTP relative to neg- ative controls. When ROS generation was examined, the results showed that pretreatment...

Ngày tải lên: 18/02/2014, 14:20

... that all b-crystallins bind calcium. Our results have important implications for understanding the calcium-related cataracto- genesis and maintenance of ionic homeostasis in the lens. Abbreviations AIM1, ... with moderate to low affinity. Stains-All and terbium-binding studies indicated that bA3-crystallin has relatively lower affinity for the cation than bB2-crystallin. Calcium-binding to...

Ngày tải lên: 18/02/2014, 16:20