Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc

... 2004 Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis Richard Zona 1, *, Florent Chang-Pi-Hin 2, *, Michael ... France Fifty-nine amino acid sequences belonging to family 57 (GH -57) of the glycoside hydrolases were collected using the CAZy server, Pfam...

Ngày tải lên: 19/02/2014, 12:20

... a PIN domain (mutMCPIP). The fragment from 412-888 bp, correspond- ing to the PIN domain, was removed from the entire cod- ing sequence of the MCPIP gene. The coding sequence lacking the PIN ... acidic residues (D141, E185, D226, D244) in PIN domains involved in binding Mg 2+ ions are conserved in all three sequences. Other conserved residues are R263, V139 and N...

Ngày tải lên: 18/02/2014, 14:20

... training corpus by calculating the weights from the frequencies of the features in the training data. 3 Japanese dependency analysis taking account of co-occurrence information and a combination ... simply adding it as a feature does not improve the accuracy. 5.5.3 Changing the amount of training data Changing the size of the training data set, we investigated wh...

Ngày tải lên: 20/02/2014, 12:20

... contains mainly syntactic in- formation, whereas CO lexical information. The other four feature sets include both syntactic and lex- ical information. SCF and DR: These more linguistically informed features ... contexts of each target verb with their part -of- speech tags. (3) keeping words in the left window only if they are tagged as a nominal. SCF+CO: We combine the SCF an...

Ngày tải lên: 20/02/2014, 09:20

... located in independent domains (a b-domain for the N-terminal cluster and an a-domain for the C-terminal cluster). In the absence of metals, apo-thionein (apo-T) is predominantly unstruc- tured and ... composed of a single polypep- tide chain of 61–68 residues. They are characterized by a conserved array of 20 cysteines and the absence of His and aromatic amino...

Ngày tải lên: 16/02/2014, 15:20

... repre- sent the last subunits incorporated into the bc 1 com- plex. On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP (Fig. 4B). Interestingly, in the ... addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc 1 co...

Ngày tải lên: 18/02/2014, 08:20

... barrel domain, including domain B and the C-terminal domain C for rBAT proteins (669 residues on average); (b) the cata- lytic TIM barrel domain, including domain B and the C-terminal domain C for ... is the lack of domain B protruding out of the TIM barrel in the place of loop 3 connecting the b3-strand to the a3-helix [9,21]. Sharing domain B by rBATs and GH1...

Ngày tải lên: 18/02/2014, 13:20

... point to tight packing of the aromatic residues in the core of the protein. From the near-UV CD signals and activity data as a function of the calcium ion concentration, two bind- ing events characterized ... estimated from isothermal titration calorimetry [36]. The C2 domains of other proteins showed cal- cium-binding affinities in the range of 1–50 lm [37,...

Ngày tải lên: 19/02/2014, 02:20

... hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of bone marrow origin, including KG1 myeloid ... the intensity for the a-tubulin signal. Shown are the relative expression levels of HO-1 and HO-2 proteins in YN-1 cells (B) and those of HO-2 protein in...

Ngày tải lên: 19/02/2014, 06:20

... activity in crude yeast extract as the untagged protein, indicating that the tag was not interfering with the protein activity. The tagged protein was purified and then used for further analysis. In the ... nkatÆmg )1 . The GLD1 pro- tein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open rea...

Ngày tải lên: 19/02/2014, 06:20