Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt

... Mg 2+ is required to perform the ATP hydrolysis. In addition, kinetic analysis had shown binding of Mg 2+ to this subunit [21]. An integrin I domain, suggested to exist in the C-terminus of the ... BchI-BchD structure the ATPase activity of BchI is blocked. A conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact...

Ngày tải lên: 19/02/2014, 12:20

... interactions by in vitro and in vivo binding experiments, using purified recombinant proteins and cultured mammalian cells expressing epitope-tagged proteins. We also investigated the effect of this interac- tion ... three-helix bundles. ESCRT-III proteins commonly contain conserved amino acid sequences for MIT binding, termed MIT-interacting motif (MIMs), in their C-termini. Two typ...

Ngày tải lên: 18/02/2014, 04:20

... pK a values of 6.0–6.6, suggesting that the catalytic histidine is protonated at pH 5. The existence of activity when the catalytic histidine is protonated indicates that the catalytic-triad mechanism of ... elimination of the bk cat activity required proto- nation of two amino acids. This biphasic titration accen- tuates the fact that the intermediate, singly protonated s...

Ngày tải lên: 20/02/2014, 23:20

... after virus infection. TRIF (adaptor pro- tein of Toll-like receptor 3)-mediated and Cardif (adaptor protein of RIG -I or MDA5)-mediated signaling pathways contribute rapid induction of interferon-b ... After dsRNA is recognized, the cytoplasmic domain of TLR3 recruits TIR-domain-containing adaptor inducing IFN-b (TRIF) through a myeloid differentiation factor 88 (MyD88)-independen...

Ngày tải lên: 18/02/2014, 16:20

... containing phenylmethanesulfonyl fluo- ride. Digitonin fractionation Isolated mitochondria (100 lg) were resuspended in SEM buffer containing indicated concentrations of digitonin (0.05% to 0.4%, ... with depleted FDXR resulted in decreased activity (Fig. 9B) that was restored by adding bovine FDX + FDXR (Fig. 9B). Additionally, the enzyme activity was inhibited by antibody to hu...

Ngày tải lên: 18/02/2014, 16:20

... inhibition element; PTB, polypyrimidine tract binding protein; RBD, RNA-binding domain; RNP, ribonucleoprotein; RRM, RNA recognition motif; SR, serine/arginine rich proteins; TLS, translocated in liposarcoma; ... nuclear ribonucleoprotein; HuD, Hu protein D; LRR, leucine rich repeat; MIF4G, middle domain of the translation initiation factor 4 G; PABP, polyadenylate binding protein; PIE,...

Ngày tải lên: 19/02/2014, 17:20

... Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE, USA SEA (sea urchin sperm protein, enterokinase and agrin) modules, minimally comprised of 120 amino acids of ... SIVV motif alone as the ratio of cleaved to uncleaved protein is greater (Fig. 4B ,D) , however, it is still highly inefficient in comparison to MUC1FDTR (Fig. 4B ,D) . Dis...

Ngày tải lên: 19/02/2014, 18:20

... Allosteric and competition equilibria involving Mn(III)heme and myristate binding to HSA. Binding sites are indicated with the notation (FA1)P(FAx)–(FAS), where FA1 is the heme binding site, acting ... Dipartimento di Biologia Strutturale e Funzionale, and Centro di Neuroscienze, Universita ` dell’Insubria, Busto Arsizio (VA), Italy 2 Dipartimento di Biologia, and Laboratorio Interdiscipl...

Ngày tải lên: 20/02/2014, 02:22

... tetramer interface. Residues lining the active site cavity are underlined. Residues binding ThDP are highlighted with a blue background, and those involved in substrate binding by yellow. Amino acids ... domains. The interface contains 10 hydrogen bonds in IPDC. The dimer–dimer interface in IPDC is smaller than the corresponding interface in ZmPDC (4400 A ˚ 2 ). It is significantly...

Ngày tải lên: 20/02/2014, 11:20

... listed in Table 1. In addition to the desired mutation, these primers also contained restriction sites, incorporated by silent muta- genesis, in order to aid selection of recombinants. The sites incorporated ... product (746 bp) containing the desired mutation was purified using a gene cleaning kit (Qiagen, Qiagen India, Genetix Biotech Asia, New Delhi, India), digested with enzyme...

Ngày tải lên: 18/02/2014, 11:20