Tài liệu Báo cáo khoa học: The conformational stability of the Streptomyces coelicolor histidine-phosphocarrier protein Characterization of cold denaturation and urea–protein interactions doc

... that the enzy- matic activity of RPE65c is iron-dependent. Characterization of the kinetic parameters of the enzymatic activity of zebrafish RPE65c To determine the steady-state kinetics of the ... the area of the 11cROL and 13cROL peaks and expressed as the mean ± SD of three independent measurements. Retinal cell dissociation The zebrafish eyes were enucl...

Ngày tải lên: 14/02/2014, 14:20

... stefin A and the different enzymes is the average distance between the centre of mass of CA atoms of the stefin molecule and the centre of mass of the CA atoms of the reactive site cysteine and histidine ... A comparison of the structure of the unli- ganded cathepsin B with the structure of the proenzyme, its complexes with chagasin and stefin A sh...

Ngày tải lên: 18/02/2014, 04:20

... inset). The absorption spectrum of the purified enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery of the activity was  10%, and the enzyme was purified 3669-fold. The ... mediated by other mammalian difla- vin reductases and also subdomains of MSR; (b) the extent of reductive remethylation of hMS catalysed by the different...

Ngày tải lên: 18/02/2014, 08:20

... 158(21) and 296(154) [14–17]. The high specific activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the ... included in the pub- lished alanine scanning mutagenesis study of FVIIa [13], and investigated the effects of the mutation on the enzymatic maturation of FVI...

Ngày tải lên: 18/02/2014, 08:20

... reaction of the luciferin ⁄ luciferase mixture, energy donor (ATP) and oxygen results in the emission of light. The intensity of the bioluminescence was detected using a luminometer, and the mitochondrial ... measured after addition of oxidative substrates, and state 3 respiration after the addition of 300 lm ADP to the electrode chamber. To test the ability o...

Ngày tải lên: 18/02/2014, 16:20

... activation of the protein kinase B survival pathway [31], p53 activa- tion [32], induction of the serum- and glucocorticoid- inducible kinase Sgk [33], expression of the heat shock protein Hsp70 [34] and ... summarizes recent progress in the understanding of how osmosensing and osmosignaling integrate into the overall context of growth factor signaling and the...

Ngày tải lên: 18/02/2014, 16:20

... following the manufacturer’s proce- dure. Each of the 18 lysine mutants of the myc–Rac1L61 sequence and the ubiquitin mutants UbR48 and UbR63 were obtained by replacing the lysine codons AAA and AAG ... binding and interac- tion with effectors. The positions of the Lys147 residues are almost identical. Lys147 of Rac1 is yellow, and the equivalent residue of...

Ngày tải lên: 18/02/2014, 16:20

... membrane and mediate interactions with the cytoskeleton and with signaling molecules. On the basis of extensive searches of the human and mouse genomic sequences, it is now known that 18 a-subunits and ... and biological function. For example, only N-glycans located on either the b-propeller of a 5 [55] or the I-like domain of b 1 or b 3 [68] contribute to the...

Ngày tải lên: 18/02/2014, 17:20

... inflammation [17], and immune response [11,12]. The study of these events is taxing, as the biosynthesis of glycoproteins is not tem- plate driven. This results in the formation of so-called ‘glycoforms’ ... proteins modulate protein activity and greatly expand the diver- sity and complexity of their biological function. The ubiquity of PTMs is reflected in their wid...

Ngày tải lên: 18/02/2014, 17:20

... handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in the AFM surface representation of Fig. 2A. By comparison with the ... kDa, and that for the c 2 ⁄ 3 monomer was 8.3 ± 0.1 kDa. Comparison of the spectra of the complete ring and the fragments revealed a stoichiometry for c 1 : c 2...

Ngày tải lên: 18/02/2014, 17:20