Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

... 271) 1747 Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding /unfolding Cheng-Yin Min, Zhi-Song Qiao and You-Min Feng Institute of Biochemistry and Cell Biology, ... protein enzymes in the B-cell granule, proinsulin is converted into insulin and C-peptide of 31 amino acids [26]. Previous studies completed on the...

Ngày tải lên: 19/02/2014, 12:20

... K562 cell line and its Pgp over-expressing subline (K562 ⁄ ADR), the GLC4 cell line and its MRP1 over-expressing subline (GLC4 ⁄ ADR), the 2008 cell line and its sublines over-expressing MRP1, ... among the resistance factor, kinetics of uptake, and kinetics of the P-glycoprotein-mediated efflux of doxorubicin, daun- orubicin, 8-(S)-fluoroidarubicin, and idarubicin in mu...

Ngày tải lên: 18/02/2014, 13:20

... : 2000 in blocking solution for 30 min. Cells were rinsed in NaCl ⁄ P i several times and incubated in blocking serum containing anti-mouse IgG (Vectastain) for a fur- ther 30 min. Staining was ... composition, indicating that the peptide is of the correct sequence and free of contaminating pro- teins. Five cycles of N-terminal sequencing confirmed the expected residues includi...

Ngày tải lên: 18/02/2014, 13:20

... but are often generic and mark masculine, feminine, or plural (Gross, 1977; Goffic and McBride, 1975). And furthermore, these same articles often are not translated into meaning preserving sentences ... The study includes off-line picture naming which forces use of articles in all of the above contexts, as well as on-line production of these morphemes in an attempt to d...

Ngày tải lên: 21/02/2014, 20:20

... second (Sox9_2) Sox9-binding site of the AP-2e promoter. Sox9 binding to both binding sites (Sox9_1 and Sox9_2) within the AP-2e promoter was observed in vivo (Fig. 5C). Finally, the direct binding of Sox9 ... of AP-2e expression at the beginning of the hypertrophic devel- opment of cartilage and of osteoarthritic chondrocytes. The dramatic increase in AP-2e expression an...

Ngày tải lên: 18/02/2014, 08:20

... depending on the disease. As a case in point, formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of ... corresponding reduction in protein stability [48,49]. However, in other studies, glycation of C-terminal lysines does not disrupt a-crystallin chaperoning [53] and activity incre...

Ngày tải lên: 19/02/2014, 18:20

... from linearity in the chevron plots of unfolding of pWT (Figs 4 and 5), which indicates that unfolding occurs in more than one step [29–31]. Reduction of autoproteolysis, in the presence of 2– 5mm ... as described below. In uence of the disulfide bridge on the kinetics of unfolding and autoproteolysis Unfolding of pWT and G8C ⁄ N60C at the standard calcium co...

Ngày tải lên: 19/02/2014, 17:20

... domains play diverse and complementary roles in SK–plasminogen complex formation, in the generation of the proteolytic active site in the plasminogen moiety and in substrate plasminogen docking and ... thermal denaturation of SK and its domains help to clarify inconsistencies existing in previous reports concerning the number of cooperative folding units in this mu...

Ngày tải lên: 21/02/2014, 03:20

... polyubiquitin chains. EMBO Rep 7, 1147–1153. 7 Feng L, Guedes S & Wang T (2004) Atrophin-1-inter- acting protein 4 ⁄ human Itch is a ubiquitin E3 ligase for human enhancer of filamentation 1 in transforming growth ... by an N-terminal C2 domain responsible for guiding intracellular localization to internal membranes, four WW domains involved in substrate recognition and a C-ter...

Ngày tải lên: 16/02/2014, 09:20

... subunits with the PRAD domain of ColQ indicates that the WAT domain of the C. intestinalis AChE is capable of organizing the sub- units into a tetramer through interaction with the PRAD domain of ... recombinant ChE from C. intestinalis expressed in vitro and native enzyme expressed in vivo indicates the enzyme is AChE To determine the nature of the cholinesterase activity of...

Ngày tải lên: 18/02/2014, 17:20