Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ- RGS3 protein Zhengding Su, Ping Xu and ... SH2 domain (i.e. pYEEI). Table 1. The effect of tyrosine phosphorylations on the binding affinity of ephrinB2...

Ngày tải lên: 19/02/2014, 12:20

... primers that incorporated BamHI and NdeI restriction sites in the 5¢-end of the amplification product, and an XhoI site in the 3¢-end. The amplification product was cloned into the TA cloning vector PCR4-TOPO ... limiting with ‘fast’ substrates. The lack of inhibition observed with benzylamine (Fig. 5A) in comparison to the inhibition observed with tryptamine (Fig....

Ngày tải lên: 19/02/2014, 07:20

... boundaries in 3.3 and combine both algo- rithms using a model ensemble to leverage indi- vidual strengths in 3.4. In 3.5 we examine how the single algorithms contribute to the result of the ensemble. In ... into training and test subsets with the ratio of 9:1 we randomly split the data into training, validation and test sets with the ratio of 8:1:1. We...

Ngày tải lên: 20/02/2014, 04:20

... one-third of the protein was cleaved at the internal site …PSNPflGPGP. The key properties of the preparation obtained were identical to those of native IF: the binding curves of vitamin B 12 to recombinant ... 7.5. Measurement of Cbl binding capacity and relative affinity of Cbl and Cbi to IF The binding capacity was measured by using 57 Co- cobalamin (...

Ngày tải lên: 21/02/2014, 00:20

... ambiguity (the word "yield" is ambiguous if there is both financial and productivity data in the knowledge base), and other complex linguistic phenomena. Let us go on to define the other ... below) and mine it very deeply, not necessarily discovering every aspect of the domain but requiring that the various aspects be integrated with one another to...

Ngày tải lên: 21/02/2014, 20:20

... for the first time, that tau interacts with Fyn -SH2. Proteins harbouring SH2 domains bind to phosphorylated tyrosines on their tar- get proteins, thereby coupling the activity of tyrosine kinases ... tau contains tyrosines that could be targeted by Fyn, and tyrosine phosphorylation in uences Fyn- SH2 binding to target proteins, we set out to deter- mine whether or not t...

Ngày tải lên: 14/02/2014, 14:20

... domain. Lanes 1–6: back- ground binding on GST beads. The binding of the different proteins to GST–SF1f was quantified (bottom) as the fraction of the input protein (% of input) that was bound to the ... U2AF 65 RS domain contributes to SF1f binding. A possible role for the U2AF 65 RS domain for binding to SF1 is consistent with the established interac...

Ngày tải lên: 19/02/2014, 07:20

... invasion. The concerted process of opening of the initial double strand and the annealing of the new duplex finally results in either the replacement of the original strand or the expulsion of the invading strand, ... determines the magnitude of peptide–RNA binding, owing to counterion release from the RNA backbone [66]. The resulting entropy increase...

Ngày tải lên: 14/02/2014, 19:20

... successful metabolic engineering of freezing tolerance in plants should include such an analysis of the dynamics of metabolism to gain com- prehensive information about the effects of individual overexpression ... metabolism in the cold. Mathemati- cal modelling and simulation of cold-induced metabolic reprogramming indicated major differences in the rates of int...

Ngày tải lên: 14/02/2014, 22:20

... All ERBB proteins have four functional domains: an extracellular ligand -binding domain; a transmembrane domain; an intracellular tyrosine kinase domain; and a C-terminal regulatory domain [2]. The ... Upon binding its ligands, dynamic conformational changes occur in both extra- cellular and intracellular domains of the receptor tyrosine kinases, resulting in the tra...

Ngày tải lên: 16/02/2014, 09:20