Tài liệu Báo cáo khoa học: Unique features of recombinant heme oxygenase of Drosophila melanogaster compared with those of other heme oxygenases studied docx

... human heme oxygenase- 1 into a peroxidase. J. Biol. Chem. 276, 10612– 10619. Ó FEBS 2004 D. melanogaster heme oxygenase (Eur. J. Biochem. 271) 1723 Unique features of recombinant heme oxygenase of Drosophila melanogaster compared ... of Drosophila melanogaster compared with those of other heme oxygenases studied Xuhong Zhang 1 , Michihiko Sat...

Ngày tải lên: 19/02/2014, 12:20

... population of monomers with a hydrodynamic radius of approximately 16 A ˚ , which is in agreement with the results of the NMR. The addition of SDS to the C-peptide sample leads to formation of larger ... presence of the indicated amount of SDS (open circles). As a control, measurements were also per- formed with a solution containing Tht only (pH 3.2) in the presence of...

Ngày tải lên: 18/02/2014, 04:20

... forms of the MAPKs were produced as GST-lofMPK3 and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 ... GST-lofMPK3 and GST-lofMPK6 were mixed with HIS-OXI1 in kinase buffer and [c- 32 P]-ATP. (B) Recombi- nant kinase-active GST-MPK3 and GST-MPK6 or recombinant kinase-inactive GST-lofMPK3 an...

Ngày tải lên: 14/02/2014, 19:20

... activity of factor VIIa by replace- ment of residues 305, 314, 337 and 374: evidence of two unique mutational mechanisms of activity enhancement. Biochem J 379, 497–503. 21 Eigenbrot C, Kirchhofer ... similar decrease in the rate of cleavage of both types of substrates. The effect of the G372(223)A mutation on K m is more con- spicuous with peptidyl substrates than with...

Ngày tải lên: 18/02/2014, 08:20

... 1223 (20–80 nm) [4] than to those of bombyxin and insulin, which are of the order of 100 pm [23,24]. The very high titer of 8K-BLP may be due to a remarkably high level of gene expression in the ... achieved. Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H] + ) (Fig. 3A)...

Ngày tải lên: 18/02/2014, 13:20

... synthetase) occurred. Thus, interaction of structural features of the tRNA with the enzyme plays a significant role in determining the accuracy of tRNA arginylation. Of the potential amino acid substrates ... affinity of the soybean synthetase for canavanine (69 lm) compared with that of the jack bean enzyme (1.3 mm), and the corresponding k cat ⁄ K M values result in discri...

Ngày tải lên: 18/02/2014, 13:20

... ERE2, ERE3 and ERE4 of the HOXC13 promoter. As seen in Fig. 5A, no significant binding of actin was observed in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERb was ... of binding of MLL1 was observed in ERE3, no significant E2-dependent binding of any of the MLLs was observed in ERE3 and ERE4 (Fig. 5A, lanes 5–8). Significant amounts o...

Ngày tải lên: 18/02/2014, 14:20

... Murakami 2 and Kenji Aoki 2 1 Division of Science of Biological Resources, Graduate School of Science and Technology, 2 Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, ... peak of the purified enzyme at 266 nm. The typical protein absorption p eak of 2 80 nm shifts to 265 nm if the protein contains a flavin-type cofac tor [28]. We failed to identif...

Ngày tải lên: 19/02/2014, 16:20

... identify subschemes of the specific biochemical network that induce instability. We then consider inte rconnections between such a subscheme a nd other parts of the kinetic scheme with or without eliminating ... each other) . The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path...

Ngày tải lên: 19/02/2014, 16:20

... activity of the enzyme with NADPH (42 UÆmg )1 ) was more than 300 times higher than with NADH (0.13 UÆmg )1 )in reductive amination of pyruvate. Oxidative deamina- tion of N-methyl-l-alanine with ... amination of pyruvate with NADPH. Fig. 1. Overexpression and purification of recombinant NMAADH. Protein samples from various stages of the purification were sub- jected to SD...

Ngày tải lên: 19/02/2014, 16:20