Tài liệu Báo cáo khoa học: Deciphering enzymes Genetic selection as a probe of structure and mechanism docx

... Monofunctional versions of the dehydratase and the dehydrogenase are provided by plasmid pKIMP-UAUC. Random gene libraries are introduced into this strain and the ability of a cell harboring an individual library ... Exploring theactivesiteofchorismatemutasebycombinatorialmutagen- esis and selection: the importance of electrostatic catalysis. Proc. NatlAcad.Sci.USA93, 5043–5048...

Ngày tải lên: 19/02/2014, 12:20

... beneficial. Because ANGPTL2 promotes vascular inflammation via the a5 b1 integrin ⁄ Rac1 ⁄ NF-jB pathway [15] and vascular injury accompanied by inflammation is con- sidered an early feature of arteriosclerosis ... resistance Chronic adipose tissue inflammation Cardiovascular disease ANGPTL2 Activation of vascular inflammation and monocyte migration Enhancement of systemic energy expend...

Ngày tải lên: 14/02/2014, 22:20

... time course of the external metabolites and the growth of biomass. In Santillan and Mackey [9], a detailed model of the lac operon was provided and analysed with respect to the bistable behaviour and ... cellu- lar networks allows the simplification of the set of equations by assuming a steady state of the intra- cellular metabolites. An approach that combines flux balan...

Ngày tải lên: 18/02/2014, 13:20

... b-1,6-GlcNAc branched tri-antennary and tetra-antennary oligosac- charides in a 5 b 1 [56]. Similarly, characterization of the carbohydrate moieties in a 3 b 1 from nonmetastatic and metastatic human melanoma cell ... M, Yamaguchi N, Kangawa K & Taniguchi N (1993) Purification and characterization of UDP-N-acetylglucosamine: alpha-6- D-mannoside beta 1-6N-acetylglucosaminyltr...

Ngày tải lên: 18/02/2014, 17:20

... (ATIS) spoken language corpus. Preliminary experiments yield 96% test set parsing accuracy. 1 Motivation As soon as a formal grammar characterizes a non- trivial part of a natural language, ... Using an Annotated Corpus as a Stochastic Grammar Rens Bod Department of Computational Linguistics University of Amsterdam Spuistraat 134 NL-1012 VB Amsterdam rens@alf.le...

Ngày tải lên: 22/02/2014, 10:20

... of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci USA 99, 11778–11783. 9 Matsuda M, Nagahama Y, Shinomiya A, Sato T, Matsuda C, Kobayashi T, Morrey CE, Shibata N, Asakawa ... Suzuki A, Kobayashi T, Paul-Prasanth B, Lau EL, Hamaguchi S, Sakaizumi M & Nagahama Y (2007) DMY gene induces male development in genetically female (XX) medaka fish. Proc Natl Acad Sci USA...

Ngày tải lên: 14/02/2014, 19:20

... vials, and centrifuged for 15 min at 16 000 g and the supernatant was transferred to new vials. A fraction was retained for determination of total protein. The remainder of the supernatant was ... cellular DA homeostasis as cellular a- syn decreases DAT-mediated DA uptake via mechanisms inhibited by PD-causing mutations [10,11]. Because of the a- syn-mediated effect on DA upta...

Ngày tải lên: 14/02/2014, 21:20

... or apoenzyme was used (data not shown). If the a, b and c subunits of the enzyme are abbreviated as a D , b D , and c D , respectively, and the a and b subunits of the reactivase are abbrevi- ated ... the enzymeÆreactivase complex was formed in small amounts from apoenzyme and the reactivase and not at all from the enzymeÆCN-Cbl complex and the reactivase (data not sh...

Ngày tải lên: 15/02/2014, 01:20

... Bishakha Shrestha, Saoni Mandal and other Mandal lab mem- bers for useful discussion and critical comments. This work was supported in parts by ARP (00365-0009- 2006) and American Heart Association ... hormones and nuclear recep- tors are intimately associated with cell differentiation, embryonic development and diverse types of human disease, including cancer and cardiovascular...

Ngày tải lên: 16/02/2014, 14:20

... neural dysfunction and death is the presence of increased neu- rotrophic activity in the AD brain. It was on this assumption that GIF was first discovered as a neuro- nal growth-inhibitory factor, and a lack of ... hallmarks of AD is the formation of b-amyloid (Ab) plaques, composed primarily of Ab(1–40) and Ab(1–42) peptides, which associate to form abnormal extracellula...

Ngày tải lên: 16/02/2014, 15:20