... the first hypothesis and therefore a direct involvement of PHI in electron transfer. This conclusion is consistent with the very low degree of identity between the N-terminal sequence of PHI and ... one PHR and one PHO (abc) protomer. The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter...
Ngày tải lên: 21/02/2014, 00:20
... is a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ion. ... to explore the isostuctural replacement of Zn(II) with Cd(II), by monitoring the peptide structure and the flexibility of Zn(II) and Cd(II) by NMR spectroscopy. An...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... cleav- age of one of the silica ester bonds, the concentration of the released product p-aminophenol was determined at a wavelength of 300 nm, which is in the trailing edge of the main absorption bands ... facilitated by proton transfer to the His residue in the catalytic center. The content of the silicasomes is released into the extracellular space [17], a...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Collecting a Why-question corpus for development and evaluation of an automatic QA-system" pdf
... tasks, and up- loads them to the MTurk Web-site. Workers can then browse the tasks and, if they find them profitable and/ or interesting enough, work on them. When the tasks are completed, the employer ... the majority of cases (83% of the questions) one of the workers replied with the NoA, the ones that answered did agree up to a point: of all the answers, 72% w...
Ngày tải lên: 20/02/2014, 09:20
Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... supported by grants 30821065 and 30870559 from the China National Nature Sciences Foundation, 2006CB910703 from the Ministry of Sciences and Technology of China, and 07dz05907 from the Committee of ... adipocytes, the translo- cation of Bax from the cytoplasm into the mitochon- dria, cytochrome c release and cleavage of caspase-3 were all observed (Fig. 4B–D)....
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... wortmannin present, the PKA inhibitor H89 further reduced the rate of thrombin generation by another 25%, thus indicating the additional involvement of PKA. To assess more directly the role of the P2Y 12 ⁄ ... was supported by the Netherlands Organi- zation for Scientific Research (902-16-276) and the Netherlands Heart Foundation (2002B014). References 1 Falati S, Gro...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... studies on the membrane binding of I C and the disposition of the I C residues that are positively charged and make up the CPY-binding sites (Fig. 4A,B), the basic residues in the vicinity of the N-terminal ... binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those of the binding of...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... v/v) as the mobile phase at a flow rate of 1.0 mLÆmin )1 and detected using an RI detector. Chito-oligomers and GlcNAc were used as standards. Further characterization of chitosan and the depolymerization ... (mL)/mass of sample (g) and the level of deacetylation was calculated by comparison between the number of free amino groups (per unit weight of the s...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Specific interaction between the classical swine fever virus NS5B protein and the viral genome pdf
... vitro the 3¢ terminal region of the minus-strand viral RNA more efficiently than the 3¢ terminal region of the plus-RNA [26]. Further, we found that the mutated plus-strand 3¢ UTR with deletion of ... peculiarities of CSFV replication because the sequence of the 3¢ terminal of 3¢ UTR is necessary fo r binding of NS5B protein and initiation of RNA synthesis. Our...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
... change the transcription level of Bcl-2 family genes and the phos- phorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members. However, each type of ... G 1 phase and a marked reduction in the proportion of cells in the S phase. The G 1 arrest and up-regulation of p27 KIP1 resulting from EGFR blockade are caused b...
Ngày tải lên: 16/02/2014, 09:20