... control was maintained in a standard incubator with 21% O2. The normoxia data are the same as shown in Fig. 1 and are included here for comparison. Expression of miR-2 7a and miR-27b at the indicated ... Biosystems ⁄ Ambion,Austin, TX, USA) were incubated in a solution containing DharmaFECT3 (Dharmacon) and then added to the con-fluent monolayer. Transfection efficiency was monitoredusing a fluorescent ... polyclonal rabbit anti-PPARc, anti-C ⁄ EBPa,anti-C ⁄ EBPb (Santa Cruz Biotechnology, Santa Cruz, CA,USA), and anti-PPARa (Zymed Laboratories, South SanFrancisco, CA, USA), and mouse monoclonal anti-b-actin(Sigma...