Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera Department ... synthesis of type 1 chain O-glycans to poly N-ace- tyllactosamine elongation and termination by sLe x .Inother cell lines and clo...

Ngày tải lên: 19/02/2014, 12:20

... cytometry. Preparation and analysis of DNA and protein extracts After the appropriate treatment, cellular DNA was isolated from 3 · 10 6 cells per sample, and analyzed for internucleo- lar fragmentation ... cytosolic fraction to the mitochondrial fraction following stea- rate administration (Fig. 3B). The presence of oleate inhibited this translocation, indicating the involvement...

Ngày tải lên: 14/02/2014, 22:20

... metalloproteinase degradation of elastin, type IV collagen and proteoglycan. A quantitative com- parison of the activities of 95 kDa and 72 kDa gelatin- ases, stromelysins-1 and -2 and punctuated metalloproteinase ... member of the MMP family, does not contain a hemopexin-like domain and acts only with its catalytic domain, and MMP-12 is special in that it autocataly...

Ngày tải lên: 16/02/2014, 14:20

... demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modu- late expression after stimulation with LP and LPS. Expression patterns of RGS1 and RGS2 after activation of TLR3 and ... activates protein kinase A. Protein kinase A phosphorylation leads to an increased expression of cyclo-oxygenase-2, also known as prostaglandin synthase-2 in HEC-1B cell...

Ngày tải lên: 18/02/2014, 13:20

... 5¢-GTAGGCATGAGAACGGGA AG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGA AA-3¢ and for CERK-negative forward 5¢-CCGCAAG AGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGA CACGGAGAT-3¢, as a negative control PCR ... interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse keratinocyte cell line upregulation of CERK expression by acti...

Ngày tải lên: 18/02/2014, 18:20

... data are in line with previous observations indicating that physiological activators of the p38 and PtdIns 3-kinase pathways including thr ombin, dexamethasone, angiotensin II and prostaglandins ... compound that induces actin polymeriza- tion by increasing actin nucleation and stabilizing actin filaments and swinholide A, a drug that sequesters G-actin as dimers [28]. As shown...

Ngày tải lên: 19/02/2014, 16:20

... 5¢-TCTCTGCTGC CAGACA-3¢ and 5¢-GCCACAGCAGAACAGA-3¢ for HVEM; 5¢-TCCTTCACCGATGGCACTATCC-3¢ and 5¢-TCAACACCAGCAGGATGCTC-3¢ for nectin-1; and 5¢-AGAAGCAGCAGCACCAGCAG-3¢ and 5¢-GTCACG TTCAGCCAGGA-3¢ for nectin-2. The 3-OST-3 ... layer of the retina and provide a potential target for viral inva- sion and permanent vision impairment. Here we examine the initial cellular and molecu...

Ngày tải lên: 18/02/2014, 14:20

... culture appears to be the simultaneous action of STAT3 and Smad in a certain ratio. Down- stream, negative regulation of differentiation pro- grammes for mesodermal and endodermal fates (mediated ... BMP signal modulation. PIAS, protein inhibitor of activated STAT proteins. Site Mechanism Example Extracellular space Modification of ligand-receptor affinity ) usually by binding t...

Ngày tải lên: 19/02/2014, 00:20

... that differ from the mammalian tachykinins by having a C-terminus FXGXRamide (X ¼ variable residues), rather than FXGLMamide and (b) invertebrate tachykinins (Inv-TKs) with an Keywords brain-gut ... muscles and intestine) using Trizol Reagent (Invi- trogen, Karlsruhe, Germany) for RNA preparation, Oligo- tex (Qiagen, Hilden, Germany) for polyA + RNA preparation and the Marathon cDNA...

Ngày tải lên: 20/02/2014, 01:20

... conformation was apparently increased. The increased lifetime of the ele- vated conformation at low Ca 2+ concentration could reasonably be a reflection of lowered ATPase activity at low Ca 2+ concentrations ... This fluctuation was strongly affected by the ATP and Ca 2+ concentrations, and was prevented by an inhibitor, thapsigargin. Interestingly, at a physio- logical ATP concentr...

Ngày tải lên: 14/02/2014, 14:20