Tài liệu Báo cáo khóa học: Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera pdf

... Suppression of b1,3galactosyltransferase b3Gal-T5 in cancer cells reduces sialyl-Lewis a and enhances poly N-acetyllactosamines and sialyl-Lewis x on O-glycans Lydia Mare and Marco Trinchera Department ... synthesis of type 1 chain O-glycans to poly N-ace-tyllactosamine elongation and termination by sLe x .Inothercell lines and clones, b3Gal-T5 transcript, b1,3Gal-T acti-vity, and sLe a antigen are also ... The main radioactive oligosaccharidesformed in BxPC3 cells (j in lower part, and A, B, and C of upper part)andT5ASclone(h in lower part, and A, B, and C of upper part)metabolically radiolabelled...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... cytometry.Preparation and analysis of DNA and proteinextractsAfter the appropriate treatment, cellular DNA was isolatedfrom 3 · 106 cells per sample, and analyzed for internucleo-lar fragmentation ... cytosolicfraction to the mitochondrial fraction following stea-rate administration (Fig. 3B). The presence of oleateinhibited this translocation, indicating the involvement of Bax activation in stearate-induced ... initiation factor 2a (eIF 2a) wasapparent after 16 h of stearate treatment, reached a peak at 22 h, and declined thereafter. Concomitantlywith eIF 2a phosphorylation, dramatic elevations in theexpression...

Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site speciﬁcities and release of matrikines pptx

... metalloproteinase degradation of elastin,type IV collagen and proteoglycan. A quantitative com-parison of the activities of 95 kDa and 72 kDa gelatin-ases, stromelysins-1 and -2 and punctuatedmetalloproteinase ... member of the MMPfamily, does not contain a hemopexin-like domain and actsonly with its catalytic domain, and MMP-12 is special in that it autocatalytically loses its hemopexin-like domainsoon after ... 100 A ˚,75lmi.d. · 150 mm; Dionex) and elution using a linear binarygradient: 5–65% ACN in 0.1% formic acid in 35 min,maintenance at 65% ACN for 10 min, and 65–5% ACN in 10 min.The typical...

Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx

... demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modu-late expression after stimulation with LP and LPS.Expression patterns of RGS1 and RGS2 afteractivation of TLR3 and ... activatesprotein kinase A. Protein kinase A phosphorylationleads to an increased expression of cyclo-oxygenase-2,also known as prostaglandin synthase-2 in HEC-1B cells [33]. We found also strong upregulation ... upregulation of both RGS mRNA.Stimulation of TLR3 and activation of the TRIF path-way leads to interferon-b production [35]. Takaokaet al. [36] demonstrated that interferon-b can inducethe transcription...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... 5¢-GTAGGCATGAGAACGGGAAG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGAAA-3¢ and for CERK-negative forward 5¢-CCGCAAGAGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGACACGGAGAT-3¢, as a negative control PCR ... interaction betweenCerK and PPARb, and its role in regulating keratino-cyte survival. We report that in a mouse keratinocytecell line upregulation of CERK expression by activa-tion of PPARb ... Mitsutake2, Urara Yokose2, Masako Sugiura3, Takafumi Kohama4 and Yasuyuki Igarashi1,21 Laboratory of Biomembrane and Biofunctional Chemistry, Faculty of Advanced Life Sciences, Hokkaido...

Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx

... data are in line with previousobservations indicating that physiological activators of thep38 and PtdIns 3-kinase pathways including thr ombin,dexamethasone, angiotensin II and prostaglandins ... compound that induces actin polymeriza-tion by increasing actin nucleation and stabilizing actinﬁlaments and swinholide A, a drug that sequestersG-actin as dimers [28]. As shown in Fig. 6A, cells ... stimuli for an additional 1 h.RNA isolation and northern blot analysisTotal RNA was extracted from cells using TRIzol Reagentfrom Invitrogen. A sample containing 12 lgoftotalRNAwas fractionated...

Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx

... 5¢-TCTCTGCTGCCAGACA-3¢ and 5¢-GCCACAGCAGAACAGA-3¢ forHVEM; 5¢-TCCTTCACCGATGGCACTATCC-3¢ and 5¢-TCAACACCAGCAGGATGCTC-3¢ for nectin-1; and 5¢-AGAAGCAGCAGCACCAGCAG-3¢ and 5¢-GTCACGTTCAGCCAGGA-3¢ for nectin-2. The 3-OST-3 ... layer of the retina and provide a potential target for viral inva-sion and permanent vision impairment. Here we examine the initial cellular and molecular mechanisms that facilitate HSV-1 invasion ... staining wasABCDE FFig. 3. Imaging and quantiﬁcation of HSV-1replication in cultured RPE cells. Conﬂuentmonolayers of RPE cells were infected withK26GFP and the viral replication was imagedat...

Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx

... culture appears to be the simultaneousaction of STAT3 and Smad in a certain ratio. Down-stream, negative regulation of differentiation pro-grammes for mesodermal and endodermal fates(mediated ... BMP signal modulation. PIAS, protein inhibitor of activated STAT proteins.Site Mechanism ExampleExtracellular space Modiﬁcation of ligand-receptor afﬁnity ) usually bybinding to ligand dimersDimeric ... signaling on one end, and TGF-b ⁄ BMP signaling on the other end.Strong activation of the BMP pathway was shownto lead to differentiation of embryonic stem cells intomesodermal and endodermal...

Tài liệu Báo cáo khoa học: Tachykinin-related peptide precursors in two cockroach species Molecular cloning and peptide expression in brain neurons and intestine docx

... that differ from the mammaliantachykinins by having a C-terminus FXGXRamide (X ¼ variable residues), rather than FXGLMamide and (b) invertebrate tachykinins (Inv-TKs) with anKeywordsbrain-gut ... muscles and intestine) using Trizol Reagent (Invi-trogen, Karlsruhe, Germany) for RNA preparation, Oligo-tex (Qiagen, Hilden, Germany) for polyA + RNApreparation and the Marathon cDNA Ampliﬁcation ... 275,23273–23280.9 Takeuchi H, Yasuda A, Yasuda-Kamatani Y, Kubo T& Nakajima T (2003) Identiﬁcation of a tachykinin-related neuropeptide from the honeybee brain usingdirect MALDI-TOF MS and its gene expression...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... conformation wasapparently increased. The increased lifetime of the ele-vated conformation at low Ca2+concentration couldreasonably be a reﬂection of lowered ATPase activity atlow Ca2+concentrations ... Thisﬂuctuation was strongly affected by the ATP and Ca2+concentrations, and was prevented by an inhibitor, thapsigargin. Interestingly, at a physio-logical ATP concentrations, the up–down motion ... physiological conditions In a buffer solution containing both 1.0 mm ATP and 100 lm free Ca2+, approximating physiological ATPconditions, SERCA molecules maintained elevatedstructures for a long...

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