Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... has Fig. 5. Analysis of the enzymatic activity of His -CoaB R206Q and A276V. The synthesis of 4¢-phosphopantothenoyl-CMP (–cysteine, upper part of the figure) and of 4¢-phosphopantothenoylcysteine (+ ... Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB Thomas Kupke Lehrstuhl fu ¨ r Mikr...

Ngày tải lên: 19/02/2014, 12:20

... example, one of the most evident patterns in the amino- acid composition of hyperthermophilic proteins is the bias against thermally labile amino- acid residues. This pattern is obvious on examination of the ... [9,47–49]. All these studies reported a decrease in the content of uncharged polar amino acids (i.e. Q, N, S, and T) and an increase in charged amino-...

Ngày tải lên: 22/02/2014, 07:20

... additional risk of oxidative stress, as DA can auto-oxidize at the neutral pH of the cytosol and thereby form toxic DA-quinone species, superoxide radicals and hydrogen peroxide. The low pH in the DA ... presence of a strong broad band centered around 75 kDa in the DAT-expressing cells that was absent in the mock-transfected cells. The broad band is probably due to th...

Ngày tải lên: 14/02/2014, 21:20

... observation). In the ternary complex, the histone H3 peptide binds in a deep chan- nel that divides a pair of acidic lobes, one of which is composed of residues from the SET-I region and the other of residues ... [23], a peptide from the Win motif of MLL1 bound to the WD40 repeat protein, WDR5 [24,25] and the C-terminal SET domain in the presence and absenc...

Ngày tải lên: 16/02/2014, 14:20

... of GIF within the brain. Further studies are required to determine the function of GIF within the normal, injured and neurodegenerative brain, and to establish the therapeutic potential of the protein. Acknowledgements This ... neurodegenerative disease. Synthesis and secretion of GIF in the brain Cellular and temporal localization of GIF synthesis Whilst the M...

Ngày tải lên: 16/02/2014, 15:20

... hydrogen bonded to the e -amino group of the Lys9 side-chain, which simultaneously interacts with the main phosphate of the substrate [32]. The closer positioning of the N-terminus to the main protein ... dehydration, together with acidification and dilution of the samples, stopped the reactions. This finding allowed the acquisition of spectra in the automatic...

Ngày tải lên: 19/02/2014, 12:20

... pyruvate, and meth- ylamine are bound to the enzyme in this order, and N-methyl-l-alanine and then NADP + are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same ... subunits. Substrate specificity and effects of various compounds on the enzymatic activity The substrate specificity of the enzyme in NADPH- dependent reductive ami...

Ngày tải lên: 19/02/2014, 16:20

... the value of 0.094 for the CRP–(cAMP) 2 complex, and no substantial changes in the shape of the emission spectra of the donor have been observed, this justifies the lack of the alteration of the ... in Trp85 as a function of cAMP concentration. F and F 0 are the fluorescence intensities of the protein in the presence and absence of the ligand, respe...

Ngày tải lên: 20/02/2014, 23:20

... independently of each other (one on the basis of the possible Eng- lish questions about the subject-domain, the other on the basis of the structure of the data base about it) there must be ... rules. The problem of simplifying the DBL* expres- sion (and other, intermediate expressions, in the full version of the PHLIQA method) can be treated separate...

Ngày tải lên: 21/02/2014, 20:20

... however, would suggest that the cavity at the protein–DNA of the nonspecific complex and the more direct association of the recognition DNA and protein surfaces of the specific complex remain unchanged ... difference in the number of included water molecules between the specific and nonspecific complexes due to a preferential hydration will depend on the natures o...

Ngày tải lên: 22/03/2014, 16:20