Tài liệu Báo cáo khóa học: The lysozyme of the starfishAsterias rubens A paradigmatic typei lysozyme docx
Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The angles around the metal cofactor sug- gested ... hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res 6, 83–101. 11 Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M,...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... cannot, however, explain the remarkably broad wings of the signal. The broad lineshape and the enhanced relaxa- tion properties of the signal at 77 K indicate that the Y Æ is coupled to a paramagnetic ... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientific & Educa...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf
... (AK; EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the aspartic acid group amino acids ... differential scanning calorimetry; EcAKIII, aspartate kinase III from Escherichia coli; MAD, multiwavelength anomalous diffraction; MjAK, aspartate kinase from Methanococcus jann...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx
... technical assistance. Drs Kazu- hito Naka (Kanazawa University) and Yasuo Ariumi (Okayama University) are also thanked for their valuable input in this study. This work was supported by Grants-in-Aid ... the inhibitory effect of NS3- 4A on the Cardif-mediated signaling pathway and the protease activity of NS3- 4A remains unclear. Furthermore, we have no evidence that all NS3-4As d...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx
... by the addition of excess unlabeled 5¢ exon product as the chase. (B) A plot of the disappearance of substrate in a normal reaction (no chase, closed circles) and reap- pearance of the substrate ... curtailed because dissociation of the cleavage product is slower than the rate of cleavage. Lastly, the results indicate that a conformational change exists between...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx
... on surface analysis, we propose a rigid domain association for the N-terminal SEA domain with the back site of the proteinase domain. Abbreviations HAI, hepatocyte growth factor activator inhibitor; ... from the refinement to calculate the R free . The final R and R free values of the model are 0.21 and 0.22 for the complete data set up to 1.6 A ˚ . The electron densit...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc
... Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al. (2003) Genome sequence of Bacillus cereus and comparative analysis with ... suggestive of a catalytic water molecule which has been replaced by the acetate moiety. The protein coordinates zinc with the N d atom of His12, one of the O d atoms of...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... that the emergence of a peak for I in the FI curves depends on the relative amplitude and rate constant of the J–I phase compared to the amplitudes and rate constants of the two other phases. The ... thylakoid membranes can be modeled by the superposition of the exponential rise to analyze quantitatively the contribution of each phase. The amplitude and rate...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina pdf
... Microcal (Northampton, MA, USA). The ther- mal denaturation of Cel7 4A was completely irreversible, and no transition was seen in a repeat scan; therefore, an apparent T m was approximated as the ... auto- docktools (ADT 1.1) was used for formatting of the ligand and macromolecule, as well as for setting the grid and docking parameters. Minor variations of the standard Lama...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khóa học: Numerical calculations of the pH of maximal protein stability pptx
... & Fersht, A. (1995) pK a values of carboxyl groups in the native and denaturated states of barnase: the pK a of the denaturated state are on average 0.4 units lower than those of model compounds. ... structural stability of patatin. J. Agric. Food Chem. 46, 2546–2553. 9. Khurana, R., Hate, A. , Nath, U. & Udgaonkar, B. (1995) pH dependence of the stability o...
Ngày tải lên: 19/02/2014, 12:20