Tài liệu Báo cáo khóa học: Inactivation of copper-containing amine oxidases by turnover products doc

... Inactivation of copper-containing amine oxidases by turnover products Paola Pietrangeli 1 , Stefania Nocera 1 , Rodolfo Federico 2 , Bruno Mondovı ` 1 and Laura Morpurgo 1 1 Department of ... consumed by an excess of amine in a closed cuvette, or the amine was consumed by oxygen in a solution stirred in air, with the rapid turnover of a thousand-fold amine ex...

Ngày tải lên: 19/02/2014, 12:20

... the formation of aggregates of the protein on exposure to dopamine [16]. In vitro experiments probably provide a better understanding of the role of various interacting components. The formation of dopamine–quinone adducts ... presence of 200 lM MPP + (E), in the presence of 100 lM MPTP and 50 lM dopamine (F), in the presence of 200 lM MPTP and 50 lM dopamine (G), in the prese...

Ngày tải lên: 14/02/2014, 19:20

... the presence of various concentrations of 2A2 mAb. The binding of 2A2 mAb was followed by native gel elec- trophoresis. Increasing the concentration of 2A2 mAb at a fixed molar ratio of cathepsin ... & Bjork I (2000) Cystatin inhibition of cathepsin B requires dislocation of the proteinase occluding loop. Demonstration by release of loop anchoring through mutation of...

Ngày tải lên: 18/02/2014, 11:20

... hydrolysis of triolein by purified recombinant rat adipocyte HSL, with a decrease in the effect of bis-ANS upon PKA phosphorylation of HSL. The interaction of HSL with bis-ANS was found to have a K d of ... typically incubated for a period of 30 min at 37 °C before the reaction was quenched by the addition of 3.25 mL of methanol ⁄ chloroform ⁄ heptane (10 : 9 : 7) and 1.1 m...

Ngày tải lên: 18/02/2014, 11:20

... presence of this enzyme in different tissues with properties of endothelial NOS, nNOS, and ⁄ or iNOS [14,31,32]. In different models of sepsis and tissues, the existence of both mtNOS and i-mtNOS isoforms ... synthesis by NOS, leading to formation of the BH 3 radical [36], and caus- ing NOS inactivation [37,38]. An alternative explan- ation for the lack of changes in nitrite u...

Ngày tải lên: 19/02/2014, 00:20

... Decma-induced Erk activation Activation of Erk by various extracellular signals is often preceded by Shc phosphorylation. Accordingly, we examined Shc activation, as indicated by its tyro- sine phosphorylation, ... indicated, followed by western blotting for Shc, phospho-Erk and total Erk levels. (C) Rescue by ectopic Shc isoform expression of Erk activation that was suppressed...

Ngày tải lên: 19/02/2014, 05:20

... stimulate some of its activities because of better substrate availability is not yet known. The partial restoration of proteasome activities by Hsps contrasts with the lack of effect induced by NAC. ... inclusions formed by httEx1-103Q. No significant modulation of inclusion size by NAC was detected. These findings confirm the results of several other studies performed in cel...

Ngày tải lên: 19/02/2014, 06:20

... result of selection. If the cutting sites are in the flexible loop of the native structure, they could prevent the selection of stable proteins. By examining the structures of the proteins studied by ... have been evolved by the assembly of nonhomologue genes. For small protein domains, they may have evolved by assembly and/or exchange of small gene segments, leading to div...

Ngày tải lên: 19/02/2014, 12:20

... synthesis of the different RNAs were obtained by PCR amplification of the first 125 nucleotides of the rpsO mRNA (5¢rpsO), the last 97 nucleotides of the same transcript (3¢rpsO), part of the 5¢ UTR of ... by PAP I M. Folichon et al. 456 FEBS Journal 272 (2005) 454–463 ª 2004 FEBS AB Fig. 2. Hfq stimulates addition of A residues by PAP I but not of C residues. (A) Addition...

Ngày tải lên: 19/02/2014, 16:20

... 343 amino acids of CSN2 and of the PCI domain of S9 ⁄ Rpn6, the paralog of CSN2 in the lid complex, did not lead to the assembly of an intact complex showing that the PCI domain of CSN2 is important ... substitution of the PCI domain of CSN2 by the PCI domain of Rpn6 has consequences for the complex integration of the chimerical CSN2-Rpn6 protein. The cDNA enco- ding the...

Ngày tải lên: 20/02/2014, 01:20