Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt

... analysis it is not obvious how the interaction between 14-3-3 and ExoS occurs, our pull-down analysis with GST-ExoS(LDL426– 428AAA) still strongly suggests that ExoS must utilize a strategy for its ... for its interaction with 14-3-3 that is similar to that seen with R18 and serotonin N-acetyltrans- ferase. This is because R18 is also nonphosphorylated and sero...

Ngày tải lên: 19/02/2014, 08:20

... genetic diseases [47]. Most analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have ... expres- sion in the face and limbs. In a follow-up study, they found that this conserved cis-acting sequence serves to maintain a level of AP-2a expression that li...

Ngày tải lên: 16/02/2014, 09:20

... admin- istration of ETA to rats, rapid endocytosis of the intact unprocessed ETA was observed, coincident with the endosomal association of the ETA-A subunit (fast association) and low molecular mass ... masses very similar to those seen with the endosomal fractions. We then assessed the major proteolytic cleavages induced by cathepsin B and ⁄ or D within the ETA seq...

Ngày tải lên: 18/02/2014, 04:20

... of the Y17 kinase activity found in HeLa lysates. C-YES is a 62 kDa nonreceptor tyrosine kinase of the Src family that is expressed in a wide range of tissues [24]. The N-terminus of C-YES is ... proce- dures. (B) Recombinant purified Src family kinases C-YES, C-SRC and LYN were assayed for Y17 kinase activity using the tube format assay and western blotting of the...

Ngày tải lên: 18/02/2014, 18:20

... Australian Postgraduate Awards administered through the University of Sydney. The authors thank Professor Roger T. Dean and Associate Professor Wendy Jessup for helpful discussions, and Mr Pat Pisansarakit ... cholesteryl esters in the core of the particle from lysosomal esterases, or that impaired lipolysis of LDL lipids may block pro- teolysis of apoB [36]. This may ar...

Ngày tải lên: 19/02/2014, 02:20

... in the lysosomes and in the cyto- plasm, but also in the nucleus [19]. Lack of expression of stefin B is associated with signs of cerebellar gran- ular cell apoptosis, ataxia and myoclonus as shown ... Comparison of stefin sequences. Shown are the primary amino acid sequences of the three stefin B proteins studied and that of stefin A. The potential copper bindin...

Ngày tải lên: 19/02/2014, 06:20

... Comparison of these results with data for the isolated cytochrome shows that binding of ligands causes only small changes in the reduction potentials of the haems and their pairwise inter- actions, ... signals and perturbing the chemical shifts of the haem methyl sig- nals in intermediate redox stages. The contraction of the line widths of the NMR signals i...

Ngày tải lên: 19/02/2014, 17:20

... detect the shorter form at all with a time resolution of 50 ls. This fact suggests two possibilities: one is that the lifetime of the smaller form is < 50 ls; another is that SERCA does not ... broken, resulting in ‘double membranes’, and these flatten on the mica sur- face with a thickness of  10 nm. Unfortunately, the smallness of the vesicles and their lo...

Ngày tải lên: 14/02/2014, 14:20

... Korea Introduction Sepsis, severe sepsis and septic shock are worldwide problems and continue to be the most common causes of death in surgical intensive care units [1]. The patho- genesis of sepsis has often ... hepatic GSH ⁄ GSSG ratio during sepsis. Thus, the results of the present study suggest that ROS produced by KCs mediate the sepsis-induced decrease in CYP1A1...

Ngày tải lên: 14/02/2014, 18:20

... suggesting that Pro79 also contrib- utes to the loop rigidity, and its conformation would be strictly trans. These findings are consistent with those of Sanders et al. [155]. On the basis of thermodynamic ... charged side chains within the hydrophobic region of the edge strand and proline residues both limit interactions with other b-pleated sheet edge strands [79]....

Ngày tải lên: 14/02/2014, 18:20