Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc

... Journal compilation ª 2006 FEBS Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris Janet R. Kumita 1 , Russell J. K. Johnson 1 , Marcos J. ... secretion levels of 10 lysozyme variants shows that the low yields of these secreted proteins, under controlled conditions, can be directly correlated with a...

Ngày tải lên: 19/02/2014, 08:20

... activating the nucleophilic water m olecule) in the first step of the reaction. A carbonium ion intermediate subsequently forms, and is further attacked by the water molecule. The study of site-directed ... 20 lLof15 M ammonium hydroxide w as added to the reaction mixture t o raise mutarotation and the anomeric form of the product was determined [42,43]. Results and Dis...

Ngày tải lên: 19/02/2014, 16:20

... selection, and provides a base- line for the interpretation of the precision curves. General findings from the precision graphs are: (i) It is only useful to consider the first halves of the SLs, as the ... for the PNV data the t-test leads to a recall of over 60% when approx. 20% of the SL has been considered. In the Figures above, there are a number of po- sition...

Ngày tải lên: 20/02/2014, 18:20

... bc 1 sub-complex, but the presence of only one of these two subunits does not substitute for the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) finally occurs. These find- ings ... too small to explain the dimerization of the bc 1 complex at this stage (i.e. just after the addition of ISP and Qcr10p). On the other hand, in the t...

Ngày tải lên: 18/02/2014, 08:20

... the two VIH isomers differed by the configuration of the tryptophan at the fourth position of the sequence and, consequently, they were named VIH and d-Trp4 VIH. This assumption was further confirmed ... sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [...

Ngày tải lên: 18/02/2014, 11:20

... origin of the hcHAT proteins within the GH13 a-amylase enzyme family, as well as shed some light on the possible evolutionary events leading to separation of the heavy-chain subunit of these amino ... sequences. In the analysis, the default neighbour-joining tree made by the selecton itself was used, the selection pressure not being mapped on a three-dimensional st...

Ngày tải lên: 18/02/2014, 13:20

... in a total volume of 1 mL. The reaction was carried out at 23 °C for 30–45 min. A control reaction was stopped by the addition of 0.1 mL of solution containing 20% (w ⁄ v) ammonium sulphate and ... out by incubating the enzyme with a large excess of reagent. The reaction conditions for the modification of Asp, Glu, Cys and Ser are shown in Table 5. No significant inactiv...

Ngày tải lên: 18/02/2014, 14:20

... trophosome-specific proteins. We demonstrated the existence of carbonic anhydrase transcripts, a protein endowed with an essential role in generating the influx of CO 2 required by the symbionts. One of the transcripts ... difficult to obtain these stages in the hydrother- mal vent environment and, second, the aposymbiotic- specific expression condition could be masked by...

Ngày tải lên: 18/02/2014, 16:20

... fluctu- ation superimposed on the global motion. The quality of the fitting was statistically validated by v 2 test against the corresponding parameter distribution of Monte Carlo simulations. The individual ... J(0.87x H ) depends only on the cross-relaxation rate; that is, it is largely deter- mined by the heteronuclear NOE and thus it is most sensitive to high-frequ...

Ngày tải lên: 18/02/2014, 16:20

... iden- tify the nature of the enzyme and provide additional information about the evolution of the ChEs, including the origins of the acyl pocket, the T exon, and the asymmetric forms of ChE in the vertebrates. Results The ... is the first demonstration of the assembly of catalytic subunits of an invertebrate AChE into asymmetric forms. The evolution of...

Ngày tải lên: 18/02/2014, 17:20