Tài liệu Báo cáo khoa học: Thermal unfolding of smooth muscle and nonmuscle tropomyosin a-homodimers with alternatively spliced exons docx

... (DSC) and circular dichroism (CD) to investigate thermal unfolding of recombinant fibroblast isoforms of a -tropomyosin (Tm) in comparison with that of smooth muscle Tm. These two nonmuscle Tm isoforms ... unfolding of nonmuscle tropomyosin E. Kremneva et al. 596 FEBS Journal 273 (2006) 588–600 ª 2006 The Authors Journal compilation ª 2006 FEBS Thermal unfolding...

Ngày tải lên: 19/02/2014, 07:20

... completely degraded TIM23 and FDXR. Figure 3D represents a full view of an immu- noblot of mitochondrial and microsomal proteins developed with a combination of CYP2E1 and TIM23 antibodies. It ... treated with or without digitonin, trypsin or Triton X-100 (Tx-100; 0.2%) and probed with antibodies against human FDXR and TIM23 as indicated. (D) A full gel pattern of the...

Ngày tải lên: 18/02/2014, 16:20

... finding of these differential functions is in line with the dogma that the proteome gains functional complexity by encoding multiple isoforms of a given protein, with these isoforms having shared and ... versions of these two isoforms, as well as of the Mxi1-WR isoform that lacks the SID, and shown to give rise to proteins of the expected size expressed at similar levels (Fig...

Ngày tải lên: 18/02/2014, 16:20

... suspen- ded in 25 mL of 40% potassium hydroxide and 25 mL of methanol and refluxed for 2 h. Products were extracted with 50 mL of hexane. The hexane layer was washed with 25 mL of saturated sodium ... addition of 0.25 mL of 40% potassium hydroxide solution and 0.25 mL of methanol, the cell suspension was boiled for 5 min. Products were extracted three times with 0.2 m...

Ngày tải lên: 19/02/2014, 07:20

... addition of 100 lLof2· Kirby buffer [25] and 80 lLofH 2 O, and rRNA was obtained by precipitation after two phenol– chloroform extractions. Four micrograms of rRNA were treatedwith20lL of acetic-aniline ... and quantification of ricin A-chain variants Purified RTA (100 lg) was mixed with 100 lgofRTB (Vector Laboratories) and made up to a final volume of 2 mL with NaCl/P i c...

Ngày tải lên: 19/02/2014, 12:20

... of the CD1d protein bands indicated was performed using a Kodak Digital Science DC40 camera and its associated software. Band intensities are shown on the right and are expressed as the sum of ... staining of cryostat sections of HepG2 cells cultured without (– Fe) or with (+ Fe) iron and count- erstained with hematoxylin at ·200 original magnification. (C) Pictures show TEM...

Ngày tải lên: 19/02/2014, 16:20

... buffer pH 6.0 with different concentrations of dithiotreitol. After dilution, cystatin F was equilibrated with a twofold molar excess of papain. The residual activity of papain was measured with Z-FR-AMC. ... localization of cystatin F, its restricted and readily regulated expres- sion [18], selective and not too potent inhibition of cathepsins are all in favor of the ac...

Ngày tải lên: 19/02/2014, 17:20

... parameters of chitinase A with various substrates. The hydrolysis of chitooligosaccharides and colloidal chitin at sub- strate concentrations of 0–2 m M was carried out with 75 ng of native chitinase ... Research Scholar of the Thailand Research Fund. We would like to thank Prof. Steve C. Fry, Institute of Cell and Molecular Biology, Univer- sity of Edinburgh, Edinburgh,...

Ngày tải lên: 20/02/2014, 01:20

... con- sisted of all sw4[5-9]∗.dps files and test consisted of all sw4[0-1]∗.dps files. However, we follow (John- son and Charniak, 2004) in deleting all partial words and punctuation from the training and ... groups of features operating on words and their edit status; the latter indicated by one of three possible flags: when the word is not part of a dis- fluency or E when it...

Ngày tải lên: 20/02/2014, 04:20

... HSPs (HSP70 and HSP23) were greatly and rapidly upregu- lated in gills but slightly less and later in mantle. One inducible and two constitutive isoforms of HSP70 are synthesized in the gills and mantle ... fluidity, energetic metabolism and translational processing. Total RNA was extracted from gills and mantle of 10 oysters exposed to 13 and 25 °C for 0, 3, 7, 10, 14, 17...

Ngày tải lên: 18/02/2014, 16:20