Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc

... conclusion, given the strong evidence supporting the conserved SF1 ⁄ U2AF 65 interaction, it is likely that the regulations of the SF1 ⁄ U2AF 65 interaction by phosphorylation of SF1 on the highly conserved ... concentra- tions of mock-phosphorylated SF1f or phosphorylated SF1f with a constant concentration of U2AF 65 (1.2 lm) confirmed the enhancement of ternary compl...

Ngày tải lên: 19/02/2014, 07:20

... phosphory- lation in cells. Tyr18 of tau plays an important role in the interaction of tau with Fyn-SH2 To determine whether the tyrosine phosphorylation status of tau itself is important for its interaction ... that this interaction is dependent on tyrosine phosphorylation of tau. A small proportion of expressed Y18F, Y29F, Y197F, Y310F and Y394F tau each bound to Fyn...

Ngày tải lên: 14/02/2014, 14:20

... (A) Titration with ephrinB2(301–333)-pY304. (B) Titration with ephrinB2(301–333). The protein concentration was 0.5 m M and the peptide concentration was 1 m M . Ó FEBS 2004 Phosphorylation of Tyr304 on ... C 0 is a constant dependent on the properties of the ligand, [L] T is the total ligand concentration, [P]isthe concentration of free SH2 protein, and K d is the equilibrium...

Ngày tải lên: 19/02/2014, 12:20

... b8-strand of one monomer into a b-sheet of the other monomer. The position of the zinc ion is indicated by a circle. (B) Schematic diagram of the dimer. Each monomer is shown with a different shade of ... site. Thus, the conformational switch of the a2-helix could be of functional relevance and be associated with either an ‘open’ nonfunctional confor- mation or a ‘closed’ co...

Ngày tải lên: 19/02/2014, 00:20

... Road, London, UK Malaria is a major health hazard in tropical and sub- tropical areas around the world. In Africa alone, every year over a million children under the age of 5 years die of malaria ... cultures of P. falciparum on the expression of para- site antioxidant defense genes at the transcription level. The highest degree of G6PD-6PGL silencing achieved was 86% at the mRN...

Ngày tải lên: 19/02/2014, 07:20

... allows study of the effects of replacement of muscle exons 1a and 2a by nonmuscle exon 1b, and comparison of Tm5a with Tm5b allows the effects of exchange of exon 6 to be studied. Studies of thermal ... (1971) The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteoly...

Ngày tải lên: 19/02/2014, 07:20

... result of hydrogen bonding between the hydroxyl of Thr167 of the protein and the amino group of compound 8. No other inhibitor offered such an interaction. For compounds 5–8, the interaction of one ... interactions with residues of the active site of T. cruzi gGAPDH. Fig. 3. gGAPDH–HOP interaction profile. (A) Comparison of phosphonate and phos- phate positions of the...

Ngày tải lên: 20/02/2014, 02:21

... size of the target cluster: (i) rare people, mentioned in only one document (335 clusters of size 1); (ii)people that appear in two documents (92 clusters of size 2), often these documents be- long ... clusters of size >=3 son dataset in the WePS corpus contains a total of 100 documents, and 10 of them belong to a British politician named James Patterson. The WePS-2 corpus cont...

Ngày tải lên: 20/02/2014, 09:20

... mRNA fractions. Titration of these two fractions indicated that Mcm2 mRNA is 4–8 times more abundant in the HeLa mRNA fraction than in the WI-38 fraction. In contrast, the concentration of glyceral- dehyde-3-phosphate ... anti-Mcm4 (second), anti-PCNA (third) and anti-Ki67 (fourth) Ig. (B) Sections containing part of the boundary of CIS (left portion) and dysplasia (CIN1 of FIGO cla...

Ngày tải lên: 20/02/2014, 23:20

... termination codon had mild effects on receptor phosphorylation and glycosylation, whereas conversion of Lys650 into methionine induced strong constitutive phosphorylation of the native non- glycosylated ... intracellular retention. By contrast, TDI mutations in the ECD or disruption of the termination codon induced a much lower level of phosphorylation of only the fully glyc...

Ngày tải lên: 19/02/2014, 00:20