Tài liệu Báo cáo khoa học: Optimal observability of sustained stochastic competitive inhibition oscillations at organellar volumes pptx

... Roussel Stochastic oscillations in competitive inhibition FEBS Journal 273 (2006) 84–95 ª 2005 The Authors Journal compilation ª 2005 FEBS 95 Optimal observability of sustained stochastic competitive inhibition ... ordinary competitive inhibition with sub- strate inflow, which is only capable of damped oscillations in the deter- ministic mass-action rate equation...

Ngày tải lên: 19/02/2014, 07:20

... which indicated that the presence of CuTCPP, H 2 TMPyP or H 2 TPPS at a concentration of 2 5 m M did not affect the cell agglutination activity of the lectin. Moreover, the addition of 0.1 M lactose to ... 1.0-cm path length cells. Temperature was maintained constant (± 0 .1 °C) by means of a Peltier d evice supplied by the manufa cture r. Determination of MCL concentration The...

Ngày tải lên: 19/02/2014, 16:20

... muta- tion of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation. Detoxification of glyphosate Detoxification of the glyphosate molecule is another strategy that has ... of the longer glyphosate. The K i values with glyphosate as substrate obtained for a series of inhibitors of varying chain length sup- port that idea by demonstrating that: (a) w...

Ngày tải lên: 14/02/2014, 14:20

... unlike that of Fyn-SH3, is influenced by Fyn-mediated tyrosine phos- phorylation of tau. In particular, phosphorylation of tau at Tyr18, a reported target of Fyn, is important for mediating Fyn-SH2–tau ... control. Pervanadate is a cell-permeable inhibitor of protein tyrosine phosphatases that acts by irreversible oxida- tion of the catalytic site [27]. We have previously dem- on...

Ngày tải lên: 14/02/2014, 14:20

... microtitre plates with a Varioskan kinetic plate reader (Thermo Electron Corporation, Waltham, MA, USA). The reactions were started by addition of substrate, and the rate of substrate oxidation was ... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL )1 and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution....

Ngày tải lên: 14/02/2014, 18:20

... nuclear translocation is accompanied by S-nitrosylation of a Cys residue in CLIC4, Cys234. The S-nitrosylation of CLIC4 has been found to induce a conformational change which destabilizes the native conformation. Such ... trans- location is linked to cellular events, it is understandable that a higher level of regulation is needed relative to proteins that regularly shuttle across the n...

Ngày tải lên: 14/02/2014, 19:20

... set of novel algorithms [16] for exhaustive identification of all nucleotide subsequences present in a pathogen genome that differ by at least a chosen number of mismatches from the sequences of ... specific amplification of DENV cDNA in the presence of excess human DNA. The mass ratio of DENV to human DNA was 1 : 1000 and the molar ratio was 1 : 0.005 (a molar ratio of appro-...

Ngày tải lên: 14/02/2014, 19:20

... ribonuclease A (4). The estimated molecular mass of ApeSOD is 57.0 kDa. (B) Sedimentation equilibrium distribution of ApeSOD. The line reflects the best fit of the data and indicates that the apparent molecular ... ultra- centrifugation, rather than gel filtration, is a powerful tool for accurately determining the oligomeric struc- ture of SOD enzymes. We observed five-coordinate and six-...

Ngày tải lên: 14/02/2014, 22:20

... study, we report data on homologous expression of the nrdF gene of C. ammoniagenes strain ATCC 6872. This is the first report of the successful purification of high amounts of the native C. ammoni- agenes ... accumulation time 84 s; time constant 82 ms; (A) native at 5 K and 12.2 lW power; (B) native at 77 K, 122 lW power, the 4.8 mT line width of the first derivative of the in...

Ngày tải lên: 15/02/2014, 01:20

... (k cat ⁄ K m ) of the mutant G198P was lower than that of the WT enzyme. The overall catalytic efficiency (k cat ⁄ K m ) of the double point mutant (G194P ⁄ G198P) was similar to that of the WT enzyme and ... Gribenko AV, Patel MM, Liu J, McCallum SA, Wang C & Makhatadze GI (2009) Rational stabilization of enzymes by computational redesign of surface charge- charge interactions...

Ngày tải lên: 15/02/2014, 01:20