Tài liệu Báo cáo khoa học: Antifungal effects and mechanism of action of viscotoxin A3 docx

... different types of cell, including animal, bacterial and fungal. The aim of this study was to obtain information on the cell targets and the mechanism of action of vis- cotoxin isoform A 3 (VtA 3 ). ... Journal 273 (2006) 72–83 ª 2005 The Authors Journal compilation ª 2005 FEBS 83 Antifungal effects and mechanism of action of viscotoxin A 3 Marcela Giudici 1,2...

Ngày tải lên: 19/02/2014, 07:20

... out in the presence of excess FAD. Higher concentrations of GdmCl induce irreversible unfolding of FprA. The exact molecular mechanism ⁄ s of the denaturing action of urea and GdmCl has not yet ... the family of glutathione reductase [8], of which FprA is a member. The FprA monomer consists of two domains: the FAD-binding domain (residues 2–108 and 324–456) consisting...

Ngày tải lên: 19/02/2014, 16:20

... the curve-fitting procedure and describing the affinity of the subunits for the substrate (K S1 and K S2 )andforP i (K I1 and K I2 ), presented in Table 1 and Table 2, respectively, are independent of the Zn 2+ ion ... additional possibility of enhanced or conformationally controlled product release. The crystal structure and the reaction mechanism of APase from E. coli, sug...

Ngày tải lên: 21/02/2014, 00:20

... Wietske Sijtsma and to a number of collegues from ITK and GM for comments and discussion. References [Abeille and Godard, 1993] A. Abeille and D. Go- dard. The Syntactic Structure of French Auxil- ... the sentence will be correctly ruled out. As for (18a) and (18b) they will be handled by the left hand side and by the right hand side of LRCL2 respectively. Lexi- ca...

Ngày tải lên: 22/02/2014, 10:20

... selection of recombinants. The sites incorporated were HaeIII, NcoI and BamHI (Table 1). The PCR mix contained 200 ng of each primer, 20 ng of the tem- plate, 200 lm of each dNTP and 5 units of Taq ... Department of Biotechnology (DBT), Department of science and technology (DST), Council of Scientific and Industrial research (CSIR) and senior research fellowship from...

Ngày tải lên: 18/02/2014, 11:20

... 3E,F). Time dependence of the bactericidal activity of H4-(86–100) and ciprofloxacin In order to evaluate the rapidity of action of his- tone H4 peptides and compare it with that of the quinolone antibiotic ... a different mechanism. The concept of a possible role for ATP in the mech- anism of action of H4-(86–100) and ciprofloxacin was strengthened by the observatio...

Ngày tải lên: 18/02/2014, 14:20

... differentiation, and so on. Isoforms of numerous proteins have been studied and compared in this man- ner, including alternative isoforms of members of the p53 ⁄ p63 ⁄ p73 [23] and the Bcl2 families ... versions of these two isoforms, as well as of the Mxi1-WR isoform that lacks the SID, and shown to give rise to proteins of the expected size expressed at similar levels (F...

Ngày tải lên: 18/02/2014, 16:20

... properties of trappin-2 involve the cationic nature of the molecule, as assessed from the salt and heparin dependence of the antimicro- bial and antifungal effects. Results Antimicrobial effects of recombinant ... effect of a 5 lm concentration of trappin-2 towards C. albicans was time-dependent, reaching 92% after 6 h and 98% after 18 h (Fig. 5). Effect of NaCl and...

Ngày tải lên: 18/02/2014, 17:20

... the membrane of cells. Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of human red ... hydrophobic moments of amphipathic a-helix-C and a-helix-D of VVA1-NTD were calculated to be 0.4 and 0.57, respectively, while those of amphipathic a-helix-D¢ and a-heli...

Ngày tải lên: 19/02/2014, 06:20

... structure and gene activity. The MMTV-LTR contains potential regulatory elements which mediate transcription in the presence of glucocorti- coid ligands and in the presence of androgen, progesterone and ... nucleosome B area, protection of the nucleosome C area and repositioning of initially randomly positioned nucleosomes (Fig. 5A and B, compare lanes 1, 2, and 3, 4 and...

Ngày tải lên: 19/02/2014, 12:20