Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt

... expected sizes of the amplified bands of anserinase, CNDP-like protein and GAPDH were 530, 395 and 517 bp, respectively. S. Yamada et al. Purification and sequence identification of anserinase FEBS ... cloning of anserinase and CNDP-like protein Database search Initially we searched for a candidate for anserinase in the GenBank database by blastp using the N-terminal se...

Ngày tải lên: 19/02/2014, 07:20

... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon ... studies of the structure and binding sites of enzymes. 8. Inhibitor binding to ribonucleases. J Mol Biol 45, 491–511. 33 Ui N (1971) Isoelectric points and c...

Ngày tải lên: 14/02/2014, 22:20

... structure of cN-II showing interfaces A and B and the Mg 2+ site. The inset shows the tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown. Table 2. Effect of point ... site and phosphotransferase reaction Figure 1 shows the aligned sequences of motif I for the six intracellular 5¢-nucleotidases. Of the six enzymes, only cN-II and cN-I...

Ngày tải lên: 15/02/2014, 01:20

... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... entire protein, and are not just restricted to the active site [1,2]. Among the metabolic consequences of having HPRT deficiency are an overproduction of uric acid and elevated l...

Ngày tải lên: 15/02/2014, 01:20

... [26]. Alignments for sequence analysis were performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction ... due to the availability of whole genome sequences of a large number of microbes, which pro- vide an increasingly rich source of information to assist in the isolation...

Ngày tải lên: 18/02/2014, 08:20

... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation ... Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association (...

Ngày tải lên: 18/02/2014, 11:20

... & Buckmann AF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12-alpha-hy- droxysteroid dehydrogenase. ... isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. Biochem...

Ngày tải lên: 18/02/2014, 11:20

... and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of ... is exceptionally stable and its midpoint potentials are quite negative. This is a direct consequence of the differing stability of the oxidized, semiquinone and reduced ApoFld:...

Ngày tải lên: 18/02/2014, 11:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... Glu204 (Fig. 3A,C), and a hydrophobic interaction between Thr131 and Phe133 (Fig. 3D). The central channel of the hexamer is made up of two layers of electrostatic forces on...

Ngày tải lên: 18/02/2014, 16:20

... is formed by an insertion of  230 residues (residues 133–367) between strands D and E and a smaller insertion of  50 residues (residues 404–458) between strands F and G. The hypervariable loop, Table ... domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF a...

Ngày tải lên: 19/02/2014, 06:20