Tài liệu Báo cáo khoa học: Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans pptx

... 5909 Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus ... infer, therefore, that both enzymes are identical in their functional properties and that the TTQ cofactor is assembled correctly i...

Ngày tải lên: 19/02/2014, 07:20

... bonding between the imidazole nitrogen of the conserved His and the hydroxyl of the active-site Ser increases the nucleophilicity of the Ser oxygen, facilitating attack of the hydroxy group on the ... bonds, the concentration of the released product p-aminophenol was determined at a wavelength of 300 nm, which is in the trailing edge of the main absor...

Ngày tải lên: 18/02/2014, 16:20

... after the initial Ca 2+ peak) resulted in an almost immediate abolition of the remaining part of the Ca 2+ signal, which then reached the level as in platelets preincubated with AR-C. In the experiments, ... further examined using two structurally distinct inhibitors, wortmannin and LY294002 [21]. In the presence of ADP ⁄ P2Y 12 activity, wortmannin or LY294002 sup...

Ngày tải lên: 18/02/2014, 16:20

... domain deletions are more com- mon at either of the protein termini, and that their occurrence is slightly higher at one of the termini, depending on the number of domains in the protein and the ... definned as the length of the amino acid sequence preceding the next domain in the arrangements, expressed as the percentage of the calcu- lated average le...

Ngày tải lên: 19/02/2014, 07:20

... at the internal site …PSNPflGPGP. The key properties of the preparation obtained were identical to those of native IF: the binding curves of vitamin B 12 to recombinant IF and gastric IF were the ... B 12 analogue cobina- mide, which binds to IF with low affinity. The absorbance spectra of the vitamin bound to recombinant IF and gastric IF were alike, as was the...

Ngày tải lên: 21/02/2014, 00:20

... ambiguity (the word "yield" is ambiguous if there is both financial and productivity data in the knowledge base), and other complex linguistic phenomena. Let us go on to define the other ... officers represented in it. The system merely made no distinction between "all" and "any", and interpreted the final query to mean the But, you...

Ngày tải lên: 21/02/2014, 20:20

... invasion. The concerted process of opening of the initial double strand and the annealing of the new duplex finally results in either the replacement of the original strand or the expulsion of the invading strand, ... determines the magnitude of peptide–RNA binding, owing to counterion release from the RNA backbone [66]. The resulting entropy increase of...

Ngày tải lên: 14/02/2014, 19:20

... metabolism in the cold. Mathemati- cal modelling and simulation of cold-induced metabolic reprogramming indicated major differences in the rates of interconversion between the pools of hexoses and sucrose, ... assessment of the trajec- tory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various int...

Ngày tải lên: 14/02/2014, 22:20

... ligands, dynamic conformational changes occur in both extra- cellular and intracellular domains of the receptor tyrosine kinases, resulting in the transphosphorylation of tyrosine residues in the ... extracellular domain is subdivided further into four domains. The tyrosine kinase domain consists of an N-lobe and a C-lobe, and ATP binds to the cleft formed between...

Ngày tải lên: 16/02/2014, 09:20

... transduction; and small- molecule tyrosine kinase inhibitors, such as gefitinib and erlotinib, which target the intracellular tyrosine kinase domain of the EGFR. Furthermore, recent clinical and laboratory ... binding to the tyrosine kinase pocket of the receptor, thereby inhibiting receptor tyrosine kinase activity and EGFR signaling pathways (Fig. 1). Early clinical studi...

Ngày tải lên: 16/02/2014, 09:20