Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b and cENaC, leading to channel ubiquitination, internalization and degradation ... donor (Fig. 8A), the inhibitory effects of heparin and DPG (Fig. 8B), the fact that puriﬁed CK2 canphosphorylate bS631 and cT599 (Fig. 7A), the presence of the a subunit of CK2 in the active cytosolic ... fractions(Fig. 6), and the ﬁnding that the electrophoretic mobility of the phosphorylating kinase corresponds to that of the asubunit of CK2 (Fig. 9). However, other experimentsaiming to detect the b subunit...

Tài liệu Báo cáo khoa học: N-Methyl-L-amino acid dehydrogenase from Pseudomonas putida A novel member of an unusual NAD(P)-dependent oxidoreductase superfamily ppt

... pyruvate, and meth-ylamine are bound to the enzyme in this order, and N-methyl-l-alanine and then NADP+are released from the enzyme. Therefore, the kinetic mechanism of the enzyme is the same ... amino-acid sequences of the geneshowed high identities (97.9% and 99.1%, respectively)with those of the PP3591 gene of P. putida KT2440.Puriﬁcation and subunit structure of NMAADH The NMAADH gene ... equilibrated with the same buffer and eluted with a linear gradient of 0–0.35 mNaCl in the buffer. The enzyme fractions were collected,concentrated, and dialyzed against the standard buffer. The ﬁnal...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism remainsunclear and it is uncertain whether it has the capacityto ... II. The hood domain is mainly builtup by residues from the C-terminus and consists of atwo-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... interacts with bothO6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro-gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the riboseare involved...

Tài liệu Báo cáo khoa học: Structural features of proinsulin C-peptide oligomeric and amyloid states pptx

... distribu-tion of 100 lM proinsulin C-peptide in the presence of divalent Ca2+ and Mg2+ionsunder native conditions (B), and of 100 lMC-peptide in the presence of insulin (C) and of 100 lM ... SDS to structurally character-ize the oligomerization process of C-peptide and ana-lyzed the formation of oligomers and their secondarystructure by complementary methods, including the detection ... and a widerange of spectroscopic techniques.Suitable methods for investigation of the secondarystructure and morphology of such structures, however,require the presence of large amounts of...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... hinge domains The composition of the two hinges that connect the FMN subdomain in NOS enzymes (H1 and H2 inFig. 5) deﬁnes the allowable movements of the FMNsubdomain and thus controls the FMN–NOSoxy ... further test the validity, kinetics and thermodynam-ics of the through-heme pathway in NOS enzymes.ConclusionsAlthough the NOS ﬂavoprotein domain has fundamen-tal structural, thermodynamic and ... changing the ratio or speed of k1 and k2,changing the rate of FMNH2formation or by a combi-nation of these effects. In addition, further tuning couldbe achieved if the changes in the FMN...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... Klinman and cowork-ers to study the viscosity dependence of the rate of hydride transfer in GO [70,71]. These authors studiedvarious glycoforms of the enzyme (varying in the extent of glycosylation) ... Comparison of rates and kinetic isotope effects using PEG-modiﬁedvariants and glycoforms of glucose oxidase: the relationship of modiﬁcation of the protein envelopeto C–H activation and tunneling. ... Stopped-ﬂow traces of the observed FMN reduction during the reaction of the wild-type (wt) and N189A mutant of MR withNADH. The wt trace is ﬁt to a single exponential and the N189Atrace to...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... orientation of the N5 of the ﬂavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of the C-terminal Tyr appears to be required for the interaction to occur [117,118]. The ... because the semiquinone H-bond with the CO of Asn is weaker than the bond formed with the sma-ller Gly, and because of the presence in the oxidizedstate of a N(5)–HN59 H-bond that is absent in otherFlds. ... protonation – the N5 position.Electron transfer from PSI toﬂavodoxinFd and Fld differ in size and in the chemical nature of their redox cofactors. There is no sequence homologybetween them, but structural...

Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx

... N10 and E97 of the secondsubunit. The backbone NH group of R98 forms ahydrogen bond with the backbone CO of F102. Fur-thermore, the orientation of the side chain of the tworesidues brings the ... nature. The growing body of sequence and structural data on these well-studiedenzymes affords an opportunity to evaluate the conse-quences of mutations. In the case of TIM, only nine of the 220–250 ... R99 (equivalentto R98 of PfTIM and yeast TIM). The shortest distancefrom the sulfur atom of M103 to the NH1 nitrogen of the guanidium group of R99 is 3.23 A˚, suggestive of apotentially stabilizing...

Tài liệu Báo cáo khoa học: Structural and functional studies on a mesophilic stationary phase survival protein (Sur E) from Salmonella typhimurium ppt

... indicate the starting and ending residues of the secondary structural elements. (C) The domain-swapped dimer of St SurE. The A and B subunits areshown in green and blue, respectively. The darker and ... as in archaic and thermophilic homo-logues. The St SurE monomer consists of 13 b-strands,six a-helices and three 310-helices. The core of the pro-tein is made up of a nine-stranded b-sheet ... the majority of the residues. There wereno disordered regions in the map. The quality of reﬁnement of the two crystal forms is shown inTable 1. Cis peptides were found between R81 and P82 and...

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... to the side chain of Asp70, and onefrom the backbone N of Gly63, and the phosphategroup forming hydrogen bonds from O1 to Arg55,from O2 to the backbone N and the side chain of Thr138, and ... to the three lysines (Fig. 3E). There is no water molecule ﬁx-ing the three lysines from the B, C and F subunits, and there is no direct interaction between subunits A and F, B and D, or C and ... the development of antimicrobial agents, and it is of greatimportance to study the structure and function of theseenzymes.E. coli, Bacillus subtilis and St. pneumoniae UMPKsare hexamers and...

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