Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio Sapora 3 , Vincenza Viti 1 and Laura Guidoni 1 1 Dipartimento ... irradiated: black) and A ⁄ (Lys + Ala) (controls: dotted white; irradiated: dotted black) as obtained from 1D and 2D COSY spectra of HeLa and MCF-7 cell samples. Data are the mea...

Ngày tải lên: 18/02/2014, 13:20

... (5¢-GCATAGCGATGTGGACGA-3¢) and QaCgClp2 (5¢-GAGGACCGAGACCGTGAA-3¢). The abbreviations ‘Qs’ and ‘Qa’ refer, respectively, to sense and antisense primers. Accurate amplification of the target amplicon was checked by obtaining ... and is characterized by the degeneration of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produ...

Ngày tải lên: 19/02/2014, 00:20

... (Promega), followed by assay of DNase I and b-galactosidase activities, according to a previously described method [32]. Preparation of nuclear extracts and EMSA The nuclear extract and probe were prepared as reported previously ... cells and a primer set of the forward pri- mer DN-5R and the reverse primer DN+854X (sequences 5¢-CG GAATTCTCAGGATGAGGGGCATGAAG-3¢ and...

Ngày tải lên: 19/02/2014, 06:20

... Characterization of the interaction between the plasma membrane H + -ATPase of Arabidopsis thaliana and a novel interactor (PPI1) Corrado Viotti, Laura Luoni, Piero Morandini and Maria Ida ... plasma membrane receptor and the activation of the plasma membrane H + -ATPase. IV. Fusicoccin induces the association between the plasma mem...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-CTTTAACTTGTTGGGCACTGG CATTG-3¢; NP6, 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried ... endo- and exo- peptidases are widespread in nature and found in viruses, archaea, bacteria and euk- aryotes. The biological importance of peptidases are clearly indicated by t...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-ATA CCATGGACAAAACCCACAGTACAATG P1OH 5¢- CATATGCACAAAACCCACAGTAC P2O 5¢-TAT GGATCCTTAATTAGAAACAAACTGTCTGA TAAAC P2OH 5¢- CTCGAGATTAGAAACAAACTGTCTGATAAACC P1Z 5¢-ATA CCATGGCTGGAGAAGACTTTAAGATC P1ZH ... 5¢- CATATGACTGGAGACTTTAAGATC P2Z 5¢-TAT GGATCCTCACCACCCAATTTCGGAAAG P2ZH 5¢- CTCGAGCCACCCAATTTCGGAAAGT Table 2. Over-expression plasmids for Sno1p and Snz1p prepared in this study. Symbols O...

Ngày tải lên: 19/02/2014, 12:20

... Aiba Y, Nakamura K, Namba T, Hirata M, Mazda O, Katsura Y & Narumiya S (1993) Thromboxane A2 receptor is highly expressed in mouse immature thymocytes and mediates DNA fragmentation and apoptosis. ... Ireland The prostanoid thromboxane (TX )A 2 induces activa- tion and aggregation of platelets, constriction of vascu- lar (V) and bronchial smooth muscle (SM) and of rena...

Ngày tải lên: 19/02/2014, 16:20

... asso- ciated with the plasma membrane of epidermal cells. At this magnification, the vacuolar membrane and the plasma membrane could not be resolved, because the vacuole takes a large part of the ... Àðk A =k 0 Þ= A ð1Þ where k A and k 0 are the inactivation rate constants either in the presence or in the absence of various concentrations of a lig...

Ngày tải lên: 20/02/2014, 01:20

... tubingensis (CAA68128.1), Pgx2 Arabi- dopsis thaliana (AAF21195.1). The mode of action (endo or exo) and the amount of GalpA cleaved off, respectively, are annotated in paren- theses. A question mark indicates ... of pectin are classified as members of family 28 of the glycoside hydrolases, including the endopolygalacturonases, exo- polygalacturonases and rhamnogalact...

Ngày tải lên: 20/02/2014, 03:20

... I domain of CD1 1a, and partially but significantly inhibited the interaction between ICAM-4 L cells and CD1 1a I domain. The adhesion of all ICAM transfectants to the I domain of CD1 1a was almost ... domain GST fusion proteins migrated as major bands of  50 kDa. After Factor X or thrombin cleavage and removal of GST the I domains appeared as single major bands...

Ngày tải lên: 20/02/2014, 11:20