Tài liệu Báo cáo khoa học: Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase docx

... 2006 The Authors Journal compilation ª 2006 FEBS Transient silencing of Plasmodium falciparum bifunctional glucose-6-phosphate dehydrogenase) 6-phosphogluconolactonase Almudena Crooke 1, *, Amalia ... molecular phenotypic effect of using this vector. This is the first report of the detection at the molecular level of the toxic effect of a DNA vector in P. falciparum....

Ngày tải lên: 19/02/2014, 07:20

... move- ment of the cofactor-binding domain of subunit A rela- tive to subunits B and C (not shown). This domain alteration involves a rotation of 5° and translation of 0.7 A ˚ . Five segments of polypeptide ... comparing the cofactor domains of the OaPDH and TbPDH structures [13]. The superposition of sub- unit A of complex I onto subunits A, B, C of complex II and III yield...

Ngày tải lên: 19/02/2014, 05:20

... there are two sentences in each of the 454 (1) kono software-no riten-ha hayaku ugoku koto this software-POST advantage-POS T quickly run to The advantage of this software is to run quickly. (2) ... the polarity of words There are some works that discuss learning the po- larity of words instead of sentences. Hatzivassiloglou and McKeown proposed a method of learning the polarity...

Ngày tải lên: 20/02/2014, 12:20

... advantages of transient interac- tions are a broad spectrum of substrates and the rapid availability of the protein for subsequent reactions. In order to avoid the general impairment of important ... basis of the mode of action. By presenting stretches of positively charged amino acids that are positioned in defined spatial config- urations, RNA chaperones enable the RNA backbon...

Ngày tải lên: 14/02/2014, 19:20

... prob- ably because of the transient nature of the interaction in solution (Fig. 1). The recent assignment of the NMR spectrum of the PCNA ring [6,7] provides the basis for solution studies of its interactions ... pace of biomolecular structure determination has provided a plethora of protein structures, but not as many structures of nucleic acids or of their complexes with...

Ngày tải lên: 14/02/2014, 19:20

... degeneration of the caudate nucleus, putamen, and occasionally the globus pallidus, with little involve- ment of the rest of the brain. The question of how mutations in the same gene or group of genes, ... periphery upon silencing [33,34]. Additionally, several experiments in various model systems have shown that the translocation of chromatin regions to the nuclear periphery re...

Ngày tải lên: 19/02/2014, 02:20

... molecular mode of action of glypho- sate and the molecular basis for glyphosate resistance. Structure and function of EPSPS EPSPS catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate. A dec- ade later, the cry...

Ngày tải lên: 14/02/2014, 14:20

... of tau with Fyn-SH3. We also show that tau interacts with the SH2 domain of Fyn, and that this associa- tion, unlike that of Fyn-SH3, is influenced by Fyn-mediated tyrosine phos- phorylation of ... SH3 domain of Fyn binds to proline-rich motifs within the sequence PXXP in interacting pro- teins. Seven such motifs are present in the proline-rich domain of the longest isoform of ta...

Ngày tải lên: 14/02/2014, 14:20

... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL )1 and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens ... buffer at pH 6.0 (25 °C). Structural formulas of the substrates are presented. Redox potentials (E°)ofT1 coppers of the laccases and redox potentials of the substrates at pH 4.5 and...

Ngày tải lên: 14/02/2014, 18:20

... reservoir consisted of 500 lL of 0.7 m sodium citrate, 10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Drops consisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL )1 , 0.75 lL of CLIC4 NLS peptide ... Luria–Bertani medium containing 30 lgÆmL )1 of kanamycin. Flasks of 400 mL of Luria– Bertani medium (30 lgÆmL )1 kanamycin) were then inocu- lated with 10 mL of the overnigh...

Ngày tải lên: 14/02/2014, 19:20