Tài liệu Báo cáo khoa học: Cytochrome P450 Cyp4x1 is a major P450 protein in mouse brain doc

... Pwo polymerase (Roche Diagnostics GmbH, Mannheim, Ger- many) and primers rluD114::cat(pKD3) 5¢ (5¢-GCT ACA ATA GCA CAC TAT ATT AAA CGG CAA AGC CGT AAA ACC CC G TGT AGG CTG GAG CTG CTT CG- 3¢) and rluD114::cat(pKD3) ... rluD114::cat(pKD3) 3¢ (5¢-GAC CAG ATT AAT GTG AAA AGA AAA TCA CGC GTA CCG GAT CGT CTT G AT GGG AAT TAG CCA TGG TCC-3¢) (comple- mentary regions to the rluD-flanking regions are...

Ngày tải lên: 18/02/2014, 16:20

... been identified in mammalian species so far [6]. Each subunit possesses an extracellular N-terminus (ligand binding domain), four transmembrane domains, an intracellular loop and an extracellular C-terminus. ... AChBP AChBP is not an ion channel (it is a soluble protein that lacks the transmembrane/intracellular parts compared to nAChRs), but importantly displays many nAChR prop- ert...

Ngày tải lên: 19/02/2014, 12:20

... Science and Information Engineering National Central University National Central University Ming Chuan University Taoyuan, Taiwan Taoyuan, Taiwan Taoyuan, Taiwan bcbb@db.csie.ncu.edu.tw yang@cl.ncu.edu.tw ... 2005). The factor C in (1) is a parameter that allows one to trade off training error and mar- gin. A small value for C will increase the number of training errors. To det...

Ngày tải lên: 20/02/2014, 12:20

... cell-permeable caspase inhibitor zVAD-fmk. PARP, a DNA repair-related enzyme, is an important substrate of caspase-3, and is cleaved from a 116-kDa protein to an 85-kDa fragment and inactivated during apoptosis ... pro-apoptotic signals and results in cleavage of a set of proteins, resulting in disassembly of the cells. Caspase-3 is a major effector caspase, and induces...

Ngày tải lên: 21/02/2014, 03:20

... showing that Cyp4x1 is a major brain P450. Immunohistochemical localization of the Cyp4x1 protein in brain showed specific staining of neurons, choroids epithelial cells and vascular endothelial cells. ... RNAs has been examined, there is no data as to whether the corresponding protein is translated in vivo. An antibody specific for the mouse Cyp4x1 protein was...

Ngày tải lên: 19/02/2014, 07:20

... Cytochrome b 559 content in isolated photosystem II reaction center preparations Inmaculada Yruela 1 , Francisca Miota 1 , Elena Torrado 1 , Michael Seibert 2 and Rafael Picorel 1 1 Estacio ´ n ... membrane fragments used as the RC-isolation starting material, as different batches of membranes obtained from spinach harvested at different seasons of the year or those from sugar beets grown...

Ngày tải lên: 20/02/2014, 11:20

... cysteine proteases [Erv -A, -B and –C (isolated from the latex of Ervatamia coronaria in our laboratory) and papain from Carica papaya (Merck, Kenilworth, NJ, USA)], two serine proteases [trypsin and ... GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M, Jagannadham MV & Dattagupta JK (2004) Structural basis of the unusual stability and substrate specificity of ervatamin-C, a plant cyst...

Ngày tải lên: 14/02/2014, 14:20

... the participating Table 1. Amino acid frequencies (%) in a- amylase linkers, in a set of globular proteins, in the Swiss-Prot databank and in intrinsically unstructured proteins. Amino acid Linkers a Globular proteins b Swiss-Prot c Intrinsically unstructured proteins b Ala ... sequence and genome databanks. Interestingly, this domain was found in some closely related bacterial...

Ngày tải lên: 14/02/2014, 18:20

... Characteristics of the importin -a: CLIC4 NLS peptide interaction. NLS residue Interactions a Van der Waals’ contact area (A ˚ 2 ) b Buried surface area (A ˚ 2 ) RSCC c Main chain Side chain Main chain ... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma- tional rearrangement that exposes the NLS in an extended conformation. Database Structural data are...

Ngày tải lên: 14/02/2014, 19:20

... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on the basis of the Michaelis–Menten equation; the maximum activity ... Schematic structures of GTF-I and its variant proteins. The proteins used start at Asp85 of GTF-I and terminate at Ser1085 and Asn1592 for GS and GSGB, respectively. B...

Ngày tải lên: 14/02/2014, 22:20