Tài liệu Báo cáo khoa học: Effects of sequestration on signal transduction cascades docx

... investigated the effects of sequestration in a complex signal transduction cascadewith negative feedback as described below.The effect of sequestration in MAPK signal transduction cascadeThe ... andMAPKK.DiscussionThe function of the signal transduction network is tosense changes in the environment of the organism in theform of signals of physicochemical origin, e.g. concen-trations of molecules ... concentrationand total kinase concentration, respectively, and KMisthe Michaelis–Menten constant of the kinase. The con-centration of the complex [TK] approaches the totalconcentration of...

Tài liệu Báo cáo khoa học: Effect of gadolinium on the ryanodine receptor/ sarcoplasmic reticulum calcium release channel of skeletal muscle docx

... these ions on Ca2+-binding or calcium ion conduct-ing membrane proteins. Lanthanide ions were found toinhibit the twitch of toad skeletal muscle with effect-iveness depending on the ion radius. ... about the effect of theluminal (trans) calcium on the activity of the RyR1 arecontroversial depending on experimental conditions.Inhibition of the channel was described at high luminalcalcium ... al. have found activation of the channel at100–250 lm and inactivation at higher luminal calciumconcentrations [15,16]. As the effect of the luminal cal-cium concentration was voltage-dependent...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... a dual action, i.e. ADP-ribosyla-tion of EF-2 (inhibition of protein synthesis) andmitochondrial alteration (intrinsic apoptotic effect).Both pathways require the translocation of ETA intothe ... cyto-solic EF-2 on the one hand, and the mitochondrialmembrane on the other hand. However, the potentialrole (if any) of ADP-ribosylation of EF-2 in themitochondrial apoptotic response induced ... C-terminal region of chol-era toxin [22]. As it was unknown whether antibodiesPlasma membranesNonreducing conditions_515306090(min, postinjection)_515306090EndosomesNonreducing conditionsETAETAETA-AETA-AReducing...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... couldreasonably be a reﬂection of lowered ATPase activity atlow Ca2+concentrations [25]. Thus, the conformationalchange from the elevated conformation to the shorterconformation was dependent on ... ﬂuctuation and shape changes correspondto the conformational changes (long-distance move-ment of the N-domain and rotational motion of theA-domain) of SERCA during the ATP-mediated iontransport ... compilation ª 2011 FEBSaddition of TG to the buffer solution. Considering thecrystallography data and the fact that, under normalbuffer conditions, the SERCA reaction usually goes inone direction...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... weredecreased by depletion of KCs. Our ﬁndings suggest that ablation of KCsprotects against hepatic drug-metabolizing dysfunction by modulation of the inﬂammatory response.AbbreviationsALT, alanine ... based on the ﬁndings of other investigators showing thedestruction of KCs after the intravenous administra-tion of GdCl3[15]. Hardonk et al. [15] demonstratedthat large KCs were no longer ... providingan early recognition of pathogen invasion and a facili-tation of the body’s subsequent immune responses [27].The stimulation of these receptors activates inﬂamma-tory responses characterized...

Tài liệu Báo cáo khoa học: Mechanisms of amyloid ﬁbril formation – focus on domain-swapping doc

... theactivation energy of 28 ± 3 kcalÆmol)1observed forthe P79S mutant tetramer formation is consistent withthe contribution of one proline isomerization event,most likely the conversion of Pro74 ... (2000)Nucleated conformational conversion and the replica-tion of conformational information by a prion determi-nant. Science 289, 1317–1321.99 Wood SJ, Wypych J, Steavenson S, Louis JC, CitronM & ... destabilization of the a conformation as a result of peptide dipolemoments. Alternation of the screening of backboneelectrostatic interactions by side chains causes differentconformational preferences...

Tài liệu Báo cáo khoa học: Modulation of a-synuclein aggregation by dopamine in the presence of MPTP and its metabolite pptx

... the conversion of MPTP toMPP+is not required for aggregation of a-synuclein. MPP+was found toaccelerate the rate of a-synuclein aggregation even in the absence of com-ponents of mitochondrial ... lm MPP+alone (0.103 h)1).The presence of dopamine, along with MPP+, resultsin a faster rate of formation of protoﬁbrils (nucleationphase) and a slower rate of conversion of protoﬁbrils ... MPTP,the presence of MPP+slowed the rate of nucleation of a-synuclein (82.3 h versus 74.9 h for a-synuclein alone)and the kinetics of ﬁbrillation was slower at a higherconcentration of the metabolite....

Tài liệu Báo cáo khoa học: Modulation of F0F1-ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels pptx

... Quantiﬁcation of the contribution of various steps to the control of mitochondrial respira-tion. J Biol Chem, 257, 2754–2757.46 Kholodenko BN (1984) Control of mitochondrialoxidative phosphorylation. ... mitochondria. Yet, inTable 2. Effect of CYPD ablation or its inhibition by cyclosporin A on the rates of respiration of mouse liver mitochondria. ACI, accep-tor control index, the rate of respiration ... CATRCATRm;where CATR is the concentration of CATR added,CATRm is the minimal concentration of CATR thatcorresponds to maximum ANT inhibition (205 nm of CATR) and aANTis the activity of ANT normalized...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... alteration of host-cell transcription signalling pathways.Another important consequence of phosphorylation-independent CagA interactions in polarized epithelialcells is the disruption of cell–cell ... successiveevents: the induction of cell scattering and cell elonga-tion. Although induction of early cell motility mainlydepends on a CagA-independent T4SS factor [23], cellelongation is clearly triggered ... various signalling cascades associated withcell polarity, cell proliferation, actin-cytoskeletal rearrangements, cell elongation, disruption of tight and adherens junctions, pro-inﬂammatoryresponses...

Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf

... to varying concentrations of stea-rate (18:0), oleate (18:1), or mixtures of the two fatty acids, and the effects on cell proliferation, lipid droplet accumulation and induction of endoplasmicreticulum ... addition, phosphorylation of eIF2a, which is characteristic of PERK branch activa-tion of ER stress, has been demonstrated in humanswith NAFLD and nonalcoholic steatohepatitis [40].In conclusion, ... administrationand was completely interrupted at longer incubationperiods. Interestingly, the interruption of TAG synthe-sis preceded the appearance of toxic effects, supportingthe notion that it constitutes...

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